Quercetin

別名：Sophoretin, C.I. 75720, NSC 9221

製品コード：S2391 純度：99.01%

Quercetin, a natural flavonoid present in vegetables, fruit and wine, is a stimulator of recombinant SIRT1 and also a PI3K inhibitor with IC50 of 2.4-5.4 μM. Quercetin induces mitophagy, apoptosis and protective autophagy. Phase 4.

CAS No. 117-39-5

サイズ	価格(税別)	在庫状況
100mg	JPY 22000	国内在庫あり
1g	JPY 59500	国内在庫なし(納期7~10日)

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

文献中Selleckの製品使用例（36）

製品安全説明書

現在のバッチを見る：純度： 99.01%

99.01

Quercetin関連製品

関連ターゲット	SIRT1 SIRT2 SIRT3 SIRT6 SIRT5	もっと見る
関連製品	Selisistat (EX 527) SRT1720 HCl Sirtinol Nicotinamide Fisetin 3-TYP AGK2 OSS_128167 SRT2104 (GSK2245840) SirReal2 Thiomyristoyl Nicotinamide Riboside Chloride Salvianolic acid B SRT2183 UBCS039 Salermide SRT3025 HCl AK 7 NRD167 Inauhzin CAY10602	もっと見る
関連化合物ライブラリー	Kinase Inhibitor Library FDA-approved Drug Library Natural Product Library Bioactive Compound Library-I Bioactive Compound Library-Ⅱ	もっと見る

シグナル伝達経路

Sirtuinシグナル伝達経路

Sirtuin阻害剤の選択性比較

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	活性情報	PMID
3T3/A31	Growth inhibition assay	10 to 100 ug/mL	72 hrs	Growth inhibition of BALB/c mouse cloned 3T3/A31 cells at 10 to 100 ug/mL after 72 hrs by nigrosin assay	10096863
BGC823	Apoptosis assay	50 uM	48 hrs	Induction of apoptotic bodies in human BGC823 cells at 50 uM after 48 hrs	17997321
BGC823	Apoptosis assay	50 uM	48 hrs	Induction of apoptosis in human BGC823 cells assessed as increase in DNA fragmentation at 50 uM after 48 hrs	17997321
BGC823	Apoptosis assay	100 uM	48 hrs	Induction of apoptosis in human BGC823 cells assessed as increase in DNA fragmentation at 100 uM after 48 hrs	17997321
HL60	Antiproliferative assay	30 uM	48 hrs	Antiproliferative activity against human HL60 cells at 30 uM after 48 hrs by MTS assay	18258440
Jurkat	Function assay	50 ug/mL	1 hr	Inhibition of heat-induced HSP70 expression in human Jurkat cells at 50 ug/mL treated for 1 hr before heat induction by Western blot	19296652
HeLa	Function assay	50 ug/mL	1 hr	Enhancement of HSP27 Ser78 phosphorylation in human HeLa cells at 50 ug/mL treated for 1 hr	19296652
Jurkat	Function assay	50 ug/mL	1 hr	Inhibition of HSF1 binding to heat shock element DNA assessed as HSF1/HSE complex formation in human Jurkat cells at 50 ug/mL treated for 1 hr before heat induction	19296652
Jurkat	Function assay	50 ug/mL	1 hr	Inhibition of of heat-induced increase in nuclear HSF1 protein phosphorylation in human Jurkat cells assessed as decrease in protein mobility at 50 ug/mL treated for 1 hr before heat induction by EMSA	19296652
HeLa	Function assay	50 ug/mL	1 hr	Inhibition of of heat-induced increase in nuclear HSF1 protein phosphorylation in human HeLa cells assessed as decrease in protein mobility at 50 ug/mL treated for 1 hr before heat induction by EMSA	19296652
MCF7	Function assay	0.5 uM to 10 uM	24 hrs	Induction of CYP1A1 expression in human MCF7 cells at 0.5 uM to 10 uM after 24 hrs by RT PCR and DNA electrophoresis	19601638
HCT116	Function assay	5 uM	1 hr	Cellular uptake in human HCT116 cells assessed a bright fluorescence light at 5 uM after 1 hr by fluorescence microscopic analysis	21090565
MCF7	Function assay	10 uM	5 hrs	Cellular uptake in human MCF7 cells assessed as formation of quercetin glucuronide in cell lysates at 10 uM after 5 hrs by HPLC-mass spectrophotometric analysis	21090565
MCF7	Function assay	10 uM	5 hrs	Cellular uptake in human MCF7 cells assessed as formation of quercetin sulphate in cell lysates at 10 uM after 5 hrs by HPLC-mass spectrophotometric analysis	21090565
MCF7	Function assay	10 uM	5 hrs	Cellular uptake in human MCF7 cells assessed as formation of oxidised quercetin in cell lysates at 10 uM after 5 hrs by HPLC-mass spectrophotometric analysis	21090565
MCF7	Function assay	10 uM	5 hrs	Cellular uptake in human MCF7 cells assessed as formation of methylated quercetin in cell lysates at 10 uM after 5 hrs by HPLC-mass spectrophotometric analysis	21090565
RAW264.7	Antiinflammatory assay	1 to 100 uM	24 hrs	Antiinflammatory activity in LPS-stimulated mouse RAW264.7 cells assessed as nitrite level at 1 to 100 uM after 24 hrs by Griess method, Activity=1.38μM	21353543
MDA-MB-231	Function assay	20 uM	30 mins	Increase in c-Fos protein expression in human MDA-MB-231 cells at 20 uM within 30 mins by Western blot analysis	21652208
MDA-MB-231	Function assay	20 uM	30 mins	Increase in Egr-1 protein expression in human MDA-MB-231 cells at 20 uM within 30 mins by Western blot analysis	21652208
MDA-MB-231	Apoptosis assay	20 uM	12 hrs	Induction of apoptosis in human MDA-MB-231 cells assessed as cleaved caspase-7 accumulation at 20 uM after 12 hrs by Western blot analysis	21652208
Jurkat	Function assay	145 uM	8 hrs	Inhibition of heat shock-induced HSP70 expression in human Jurkat cells at 145 uM treated 2 hrs before beat shock challenge measured after 8 hrs by Western blotting	21798748
HCT116	Function assay	25 uM	10 mins	Inhibition of PI3Kalpha in human HCT116 cells using [32P]ATP at 25 uM after 10 mins	22212721
HCT116	Function assay	25 uM	10 mins	Inhibition of PI3Kalpha H1047R mutant in human HCT116 cells using [32P]ATP at 25 uM after 10 mins	22212721
HT22	Neuroprotective assay	25 uM	24 hrs	Neuroprotective activity against glutamate induced oxidative stress in mouse hippocampal HT22 cells assessed as cell viability at 25 uM after 24 hrs by MTT assay	22624880
RAW264.7	Function assay	50 uM	3 hrs	Activation of Nrf2 in mouse RAW264.7 cells assessed as cytosolic accumulation at 50 uM after 3 hrs by Western blotting analysis	23294286
RAW264.7	Function assay	50 uM	3 hrs	Activation of Nrf2 in mouse RAW264.7 cells assessed as nuclear accumulation at 50 uM after 3 hrs by Western blotting analysis	23294286
ARPE19	Function assay	20 uM	18 days	Inhibition of intracellular A2E photooxidation in human ARPE19 cells at 20 uM after 18 days	23346866
HCT116	Cytotoxicity assay	1 to 100 uM	12 hrs	Cytotoxicity against human HCT116 cells at 1 to 100 uM after 12 hrs by MTT assay	23434132
MCF7	Cytotoxicity assay	1 to 100 uM	12 hrs	Cytotoxicity against human MCF7 cells at 1 to 100 uM after 12 hrs by MTT assay	23434132
HT22	Neuroprotective assay	40 uM	3 hrs	Neuroprotective activity in mouse HT22 cells assessed as t-BOOH-induced toxicity at 40 uM preincubated for 3 hrs followed by t-BOOH induction measured after 9 hrs by MTT assay	24245939
HT22	Neuroprotective assay	40 uM	3 hrs	Neuroprotective activity in mouse HT22 cells assessed as reduction of t-BOOH-induced oxidative stress at 40 uM preincubated for 3 hrs followed by t-BOOH induction measured after 20 hrs by MTT assay	24245939
RAW264.7	Antiinflammatory assay	10 uM	18 hrs	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion at 10 uM after 18 hrs by sandwich ELISA	24679441
HT22	Neuroprotective assay	5 uM	48 hrs	Neuroprotection against glutamate challenged mouse HT22 cells assessed as reduction in cell viability at 5 uM after 48 hrs by MTT assay	24819955
HT22	Neuroprotective assay	25 uM	24 hrs	Neuroprotective activity against glutamate-induced oxidative stress in mouse HT22 cells assessed as reduction of intracellular ROS formation at 25 uM after 24 hrs by MTT assay	24900407
PC12	Neuroprotective assay	5 uM	24 hrs	Neuroprotective activity in rat PC12 cells assessed as inhibition H2O2-induced reduction of cell viability at 5 uM after 24 hrs by MTT assay	24941128
HLF1	Function assay	2 to 5 uM	24 hrs	Inhibition of H2O2-induced oxidative stress in human HLF1 cells assessed as increase in cell viability at 2 to 5 uM pretreated for 24 hrs followed by addition of 300 uM H2O2 for 2.5 hrs	24996137
RAW264.7	Antiinflammatory assay	3 uM	24 hrs	Antiinflammatory activity in LPS-stimulated mouse RAW264.7 cells assessed as reduction in nitric oxide production at 3 uM after 24 hrs by Griess method based ELISA (Rvb = 52.4 +/- 0.9 uM), Activity=13.5μM	25106885
RAW264.7	Antiinflammatory assay	1.5 uM	24 hrs	Antiinflammatory activity in LPS-stimulated mouse RAW264.7 cells assessed as reduction in nitric oxide production at 1.5 uM after 24 hrs by Griess method based ELISA (Rvb = 52.4 +/- 0.9 uM), Activity=20.8μM	25106885
MDA-MB-231	Function assay	5 uM	16 hrs	Inhibition of PMA-stimulated NF-kappaB signaling (unknown origin) expressed in MDA-MB-231 cells at 5 uM incubated for 16 hrs by luciferase reporter gene assay	25190466
MDA-MB-231	Apoptosis assay	3.125 to 25 uM	48 hrs	Induction apoptosis in PMA-stimulated human MDA-MB-231 cells assessed as mitochondrial disruption 3.125 to 25 uM after 48 hrs by JC1 staining based fluorescence assay	25190466
HCT116	Cytotoxicity assay	1.25 to 20 uM	96 hrs	Cytotoxicity against human HCT116 cells at 1.25 to 20 uM after 96 hrs by MTT assay	25397870
HCT116	Apoptosis assay	20 uM	72 hrs	Induction of apoptosis in human HCT116 cells assessed as PARP cleavage at 20 uM after 72 hrs by immunoblotting	25397870
HCT116	Antitumor assay	80 uM	4 days	Antitumor activity against human HCT116 cells xenografted in 48 hpf zebrafish embryo assessed as inhibition of cancer cell proliferation at 80 uM treated for 4 days	25397870
BL21	Function assay	20 uM	15 mins	Inhibition of recombinant human N-terminal His6-tagged AKR1B10 expressed in Escherichia coli BL21 cells using all-trans-retinal as substrate at 20 uM incubated for 15 mins by HPLC method	26529431
HT22	Neuroprotective assay	25 uM	24 hrs	Neuroprotective activity against glutamate-induced oxycytotic cell death in mouse HT22 cells assessed as cell viability at 25 uM after 24 hrs by MTT assay	26886849
PC12	Function assay	5 uM	2 hrs	Protective effect against H2O2-induced cell death in rat PC12 cells at 5 uM preincubated for 2 hrs followed by H2O2 addition for 24 hrs by MTT assay	27343850
PC12	Antioxidant assay	2.5 uM	2 hrs	Antioxidant activity in rat PC12 cells assessed as decrease of H2O2-induced ROS production at 2.5 uM preincubated for 2 hrs followed by H2O2 addition for 24 hrs by DCFH-DA /DAPI-based fluorescence assay	27343850
LLC-PK1	Renoprotective assay	10 to 100 uM	2 hrs	Renoprotective activity in pig LLC-PK1 cells assessed as inhibition of cisplatin-induced damage by measuring increase in cell viability at 10 to 100 uM preincubated for 2 hrs followed by cisplatin addition after 24 hrs by Ez-Cytox reagent based assay	28487074
PC12	Neuroprotective assay	10 uM	3 hrs	Neuroprotective activity in rat PC12 cells assessed as decrease in H2O2 mediated apoptosis at 10 uM preincubated for 3 hrs followed by H2O2 challenge measured after 24 hrs by MTT assay	28803044
SH-SY5Y	Neuroprotective assay	100 uM	12 hrs	Neuroprotective activity against H2O2-induced cell death in human SH-SY5Y cells assessed as increase in cell viability at 100 uM pretreated for 12 hrs followed by H2O2 challenge measured after 3 hrs by MTT assay	28988761
RAW264.7	Cytoprotective assay	1 pM	2 hrs	Cytoprotective activity against H2O2-induced oxidative stress in mouse RAW264.7 cells assessed as increase in cell viability at 1 pM incubated for 2 hrs prior to H2O2 addition measured after 24 hrs by MTT assay	29681148
HCT116	Function assay	2.5 uM	30 mins	Inhibition of IP6K2 in human [3H]-inositol-labelled HCT116 cells assessed as reduction in insp7 levels at 2.5 uM after 30 mins by HPLC analysis	30624931
HEK293	Function assay	2.5 uM	3.5 hrs	Inhibition of IP6K2 in [3H]-inositol-labelled HEK293 cells assessed as reduction in insp7 levels at 2.5 uM after 3.5 hrs by HPLC analysis relative to control	30624931
3T3-L1	Function assay	2.5 uM	3 hrs	Activation of AKT activity in mouse 3T3-L1 cells assessed as increase in AKT phosphorylation at T308 residues at 2.5 uM after 3 hrs by Western blot analysis	30624931
3T3-L1	Function assay	1 uM	3 hrs	Inhibition of IP6K2 in mouse 3T3-L1 cells assessed as AKT phosphorylation at T308 at 1 uM after 3 hrs by Western blot analysis	30624931
HCT116	Function assay	2.5 uM	3.5 hrs	Inhibition of IP6K2 in human [3H]-inositol-labelled HCT116 cells assessed as reduction in insp7 levels at 2.5 uM after 3.5 hrs by HPLC analysis relative to control	30624931
CGN	Neuroprotective assay	5 to 100 uM	24 hrs	Neuroprotective activity in Sprague-Dawley rat CGN cells assessed as protection against H2O2-induced cytotoxicity by measuring increase in cell viability at 5 to 100 uM pre-incubated for 24 hrs before H2O2 stimulation for 24 hrs by MTT assay	30935792
PC12	Neuroprotective assay	10 uM	3 hrs	Neuroprotective activity in rat PC12 cells assessed as protection against H2O2-induced cytotoxicity at 10 uM pre-incubated for 3 hrs before H2O2 addition and measured after 12 hrs by MTT assay	31128990
SH-SY5Y	Neuroprotective assay	100 uM	1 hr	Neuroprotective activity against H2O2-induced oxidative stress in human SH-SY5Y cells assessed as reduction in ROS generation at 100 uM preincubated for 1 hr followed by DCFH-DA addition for 30 mins and subsequent co-treatment with compound and H2O2 for 3	31158754
HT22	Neuroprotective assay	25 uM	24 hrs	Neuroprotection against glutamate-induced cell death in mouse HT22 cells assessed as increase in cell viability at 25 uM measured after 24 hrs by MTT assay	31609115
HT22	Neuroprotective assay	25 uM	24 hrs	Neuroprotective activity at mouse HT22 cells assessed as reduction in glutamate-induced oxidative stress by measuring cell viability at 25 uM incubated for 24 hrs by MTT assay	31609608
BGC823	Function assay	50 uM		Effect on cell morphology in human BGC823 cells assessed as chromosomal condensations at 50 uM	17997321
3T3L1	Function assay	10 uM		Induction of AMPK phosphorylation in mouse 3T3L1 cells at 10 uM by Western blotting	20822902
3T3L1	Function assay	10 uM		Induction of AMPK-mediated ACC phosphorylation in mouse 3T3L1 cells at 10 uM by Western blotting	20822902
HCT116	Function assay	25 uM		Inhibition of PI3Kalpha in human HCT116 cells assessed as inhibition of Akt Ser473 phosphorylation at 25 uM by immunoblot analysis	22212721
HCT116	Function assay	25 uM		Inhibition of PI3Kalpha H1047R mutant in human HCT116 cells assessed as inhibition of Akt Ser473 phosphorylation at 25 uM by immunoblot analysis	22212721
3T3L1	Antiobesity assay	100 uM		Antiobesity activity in mouse 3T3L1 cells assessed as reduction of fat accumulation at 100 uM by oil Red O staining-based ELISA	23642481
HT22	Neuroprotective assay	25 uM		Neuroprotective activity in mouse HT22 cells assessed as reduction in glutamate-induced cytotoxicity at 25 uM by MTT assay	25059502
MDA-MB-231	Function assay	25 uM		Inhibition of basal NF-kappaB signaling in human MDA-MB-231 cells assessed as reduction in MMP9 mRNA expression at 25 uM by quantitative RT-PCR method relative to untreated control	25190466
MDA-MB-231	Function assay	25 uM		Inhibition of NF-kappaB signaling in human MDA-MB-231 cells assessed as reduction in PMA-stimulated MMP9 mRNA expression at 25 uM pre-incubated before PMA stimulation by quantitative RT-PCR method relative to untreated control	25190466
MDA-MB-231	Function assay	25 uM		Inhibition of basal NF-kappaB signaling in human MDA-MB-231 cells assessed as reduction in COX2 mRNA expression at 25 uM by quantitative RT-PCR method relative to untreated control	25190466
MDA-MB-231	Function assay	25 uM		Inhibition of NF-kappaB signaling in human MDA-MB-231 cells assessed as reduction in PMA-stimulated COX2 mRNA expression at 25 uM pre-incubated before PMA stimulation by quantitative RT-PCR method relative to untreated control	25190466
MDA-MB-231	Function assay	25 uM		Inhibition of NF-kappaB signaling in human MDA-MB-231 cells assessed as reduction in PMA-stimulated MMP9 mRNA expression at 25 uM pre-incubated before PMA stimulation by quantitative RT-PCR method	25190466
MDA-MB-231	Function assay	25 uM		Inhibition of NF-kappaB signaling in human MDA-MB-231 cells assessed as reduction in PMA-stimulated COX2 mRNA expression at 25 uM pre-incubated before PMA stimulation by quantitative RT-PCR method	25190466
PC12	Function assay	10 uM		Inhibition of human amyloid beta (1 to 42) assessed as neuroprotective activity against amyloid beta (1 to 42)-induced toxicity in rat PC12 cells by measuring reduction in cell viability at 10 uM incubated 15 mins prior to amyloid beta (1 to 42) addition	28559058
MDA-MB-468	Function assay	6.6 uM		Inhibition of human CYP1B1 expressed in MDA-MB-468 cells assessed as potentiation of cisplatin-induced cytotoxicity by measuring cisplatin EC50 at 6.6 uM (Rvb = 6.70+/- 0.7 uM), EC50=1.1μM	29150398
HEK293	Function assay	20 uM		Inhibition of human CYP1A1 transfected in HEK293 cells assessed as protection against CYP1A1 mediated B[a]P toxicity by measuring B[a]P EC50 at 20 uM by MTT assay (Rvb =1.2+/- 0.3 uM), EC50=8.8μM	29150398
L929	Function assay		2 hrs	Potentiation of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality treated with drug after 2 hrs of TNFalpha treatment	9287415
L929	Function assay		2 hrs	Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality treated with drug after 2 hrs of TNFalpha treatment	9287415
L929	Function assay		15 mins	Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by [methyl-3H]thymidine incorporation assay, EC50=26μM	9287415
L929	Function assay		15 mins	Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by crystal violet staining, EC50=20μM	9287415
SKBR3	Antiapoptotic assay		72 hrs	Inhibition of HSP70-mediated antiapoptotic activity in human SKBR3 cells assessed as induction of cell growth arrest after 72 hrs by propidium iodide staining-based flow cytometry	17603540
SKBR3	Antiapoptotic assay		24 hrs	Inhibition of HSP70-mediated antiapoptotic activity in human SKBR3 cells assessed as increase in caspase-3 activity after 24 hrs by Western blot analysis	17603540
SKBR3	Antiapoptotic assay		24 hrs	Inhibition of HSP70-mediated antiapoptotic activity in human SKBR3 cells assessed as induction of caspase-3 cleavage after 24 hrs by Western blot analysis	17603540
SKBR3	Antiapoptotic assay		24 hrs	Inhibition of HSP70-mediated antiapoptotic activity in human SKBR3 cells assessed as induction of PARP cleavage after 24 hrs by Western blot analysis	17603540
RAW264.7	Function assay		16 hrs	Inhibition of LPS and INF-alpha-induced nitric oxide production in mouse RAW264.7 cells after 16 hrs, IC50=25μM	18027905
RAW264.7	Antiinflammatory assay		24 hrs	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess reagent assay, IC50=9.6μM	19555124
RAW264.7	Antiinflammatory assay		24 hrs	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitrite accumulation after 24 hrs by Griess reagent method, IC50=11.4μM	19778086
Vero	Cytotoxicity assay		3 days	Cytotoxicity against african green monkey Vero cells after 3 days by MTT assay, MNCC<20μM	19778089
Vero	Cytotoxicity assay		3 days	Cytotoxicity against african green monkey Vero cells after 3 days by MTT assay, MNCC<20μM	20092288
RAW264.7	Antiinflammatory assay		24 hrs	Antiinflammatory activity against mouse RAW264.7 cells assessed as Inhibition of LPS-induced nitric oxide production after 24 hrs by ELISA, IC50=32.1μM	20121165
B16-4A5	Function assay		72 hrs	Inhibition of theophylline-stimulated melanogenesis in mouse B16-4A5 cells after 72 hrs, IC50=15μM	20189399
RAW264.7	Antiinflammatory assay		24 hrs	Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as inhibition of NO production after 24 hrs, IC50=34.5μM	20825224
HepG2(2.2.15)	Cytotoxicity assay		8 days	Cytotoxicity against human HepG2(2.2.15) cells after 8 days by MTT assay, CC50=38.1μM	21401115
HepG2	Function assay		24 hrs	Inhibition of oleic acid-induced triglyceride over-accumulation in human HepG2 cells incubated for 24 hrs relative to untreated control, IC30=14.9μM	21824690
HepG2	Antioxidant assay		24 hrs	Antioxidant activity in human HepG2 cells assessed as reduction of oleic acid-induced ROS generation incubated for 24 hrs by DHCF-DA based fluorimetric assay relative to untreated control, IC30=30.8μM	21824690
MDCK	Antiviral assay		4 days	Antiviral activity against influenza A virus (A/swine/OH/511445/2007(H1N1)) Oh7 infected in MDCK cells assessed as inhibition of viral replication after 4 days by quantitative RT-PCR, ED50=48.2μM	22115591
HCT116	Growth inhibition assay		48 hrs	Growth inhibition of human HCT116 cells overexpressing PI3Kalpha H1047R mutant after 48 hrs by MTT assay, IC50=5.7μM	22212721
HCT116	Growth inhibition assay		48 hrs	Growth inhibition of human HCT116 cells after 48 hrs by MTT assay, IC50=6.7μM	22212721
HCT116	Growth inhibition assay		48 hrs	Growth inhibition of human HCT116 cells overexpressing PI3Kalpha after 48 hrs by MTT assay, IC50=23μM	22212721
Huh7.5.1	Antiviral assay		72 hrs	Antiviral activity against HCV JFH-1 J399EM infected in Human Huh7.5.1 cells assessed as suppression of viral replication after 72 hrs by EGFP assay, EC50=19.8μM	22445328
MV4-11	Cytotoxicity assay		72 hrs	Cytotoxicity against human MV4-11 cells harboring FLT3 mutation after 72 hrs by tetrazolium based Ez CyTox cell viability assay, GI50=13.1μM	23411073
RS4:11	Cytotoxicity assay		72 hrs	Cytotoxicity against human RS4:11 cells harboring wild type FLT3 after 72 hrs by tetrazolium based Ez CyTox cell viability assay, GI50=21.6μM	23411073
HTLA	Function assay		20 mins	Inhibition of CX3CL1-stimulated CX3CR1 in human HTLA cells pre-incubated for 20 mins measured on day 4 by beta arrestin-recruitment mediated luciferase reporter gene assay, IC50=4.5μM	23437772
HTLA	Function assay		20 mins	Inhibition of CX3CL1-stimulated CX3CR1 in human HTLA cells pre-incubated for 20 mins measured on day 4 by beta arrestin-recruitment mediated luciferase reporter gene assay, IC50=5.01187μM	23437772
HTLA	Function assay		20 mins	Inhibition of vasopressin-stimulated vasopressin V2 receptor in human HTLA cells pre-incubated for 20 mins measured on day 4 by beta arrestin-recruitment mediated luciferase reporter gene assay, IC50=6.30957μM	23437772
HTLA	Function assay		20 mins	Inhibition of vasopressin-stimulated vasopressin V2 receptor in human HTLA cells pre-incubated for 20 mins measured on day 4 by beta arrestin-recruitment mediated luciferase reporter gene assay, IC50=7μM	23437772
HTLA	Function assay		20 mins	Inhibition of CCL22-stimulated CCR4 in human HTLA cells pre-incubated for 20 mins measured on day 4 by beta arrestin-recruitment mediated luciferase reporter gene assay, IC50=10μM	23437772
HTLA	Function assay		20 mins	Inhibition of CCL22-stimulated CCR4 in human HTLA cells pre-incubated for 20 mins measured on day 4 by beta arrestin-recruitment mediated luciferase reporter gene assay, IC50=10.2μM	23437772
HTLA	Function assay		20 mins	Inhibition of vasopressin-stimulated vasopressin V2 receptor in human HTLA cells pre-incubated for 20 mins with Tween-80-treated compound solution measured on day 4 by beta arrestin-recruitment mediated luciferase reporter gene assay, IC50=15.2μM	23437772
HTLA	Function assay		20 mins	Inhibition of vasopressin-stimulated vasopressin V2 receptor in human HTLA cells pre-incubated for 20 mins with Tween-80-treated compound solution measured on day 4 by beta arrestin-recruitment mediated luciferase reporter gene assay, IC50=15.8489μM	23437772
HTLA	Function assay		20 mins	Inhibition of vasopressin-stimulated vasopressin V2 receptor in human HTLA cells pre-incubated for 20 mins with centrifuged compound solution measured on day 4 by beta arrestin-recruitment mediated luciferase reporter gene assay, IC50=37.8μM	23437772
HTLA	Function assay		20 mins	Inhibition of vasopressin-stimulated vasopressin V2 receptor in human HTLA cells pre-incubated for 20 mins with centrifuged compound solution measured on day 4 by beta arrestin-recruitment mediated luciferase reporter gene assay, IC50=39.8107μM	23437772
RAW264.7	Function assay		24 hrs	Inhibition of iNOS-mediated nitric oxide production in LPS-stimulated mouse RAW264.7 cells after 24 hrs by Griess reaction analysis, IC50=16.4μM	23517145
RAW264.7	Function assay		24 hrs	Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 24 hrs, IC50=1.3μM	23540981
HepG2	Antioxidant assay		1 hr	Antioxidant activity in human HepG2 cells assessed as inhibition of ABAP-induced intracellular peroxyl radical scavenging activity by measuring ROS production measured every 5 mins for 1 hr by absorbance assay, IC50=11μM	24471493
HT22	Neuroprotective assay		24 hrs	Neuroprotective activity in mouse HT22 cells assessed as protection against glutamate-induced oxidative damage after 24 hrs by MTT assay, EC50=3.13μM	24602904
HT22	Cytotoxicity assay		24 hrs	Cytotoxicity against mouse HT22 cells after 24 hrs by MTT assay, EC50=11.52μM	24602904
U2OS	Function assay		5 hrs	Agonist activity at GPR35 receptor in human U2OS cells coexpressing Gal4-VP16-TEV assessed as beta arrestin translocation after 5 hrs by beta lactamase reporter gene assay, EC50=5.93μM	24900447
HT-29	Function assay		10 mins	Agonist activity at GPR35 receptor in human HT-29 cells after 10 mins by dynamic mass redistribution assay, EC50=8.02μM	24900447
HT-29	Function assay		10 mins	Desensitization of GPR35 receptor in human HT-29 cells assessed as inhibition of zaprinast-induced dynamic mass redistribution after 10 mins, IC50=15.4μM	24900447
HT-29	Function assay		10 mins	Partial agonist activity at GPR35 receptor in human HT-29 cells after 10 mins by dynamic mass redistribution assay	24900447
HL60	Cytotoxicity assay		72 hrs	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay, IC50=47μM	25014747
BV2	Function assay		24 hrs	Inhibition of LPS-stimulated NO production in human BV2 cells after 24 hrs by Griess assay, IC50=4.3μM	25127164
RAW264.7	Anti-inflammatory assay		24 hrs	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of lipopolysaccharide-induced nitric oxide production incubated for 24 hrs by Griess reagent based assay, IC50=16.6μM	25245917
C6	Function assay		72 hrs	Inhibition of cell proliferation of rat C6 cells assessed as cell viability after 72 hrs by sulforhodamine B assay, IC50=18.38μM	25442304
HCT15	Cytotoxicity assay		72 hrs	Cytotoxicity against human HCT15 cells assessed as cell growth inhibition after 72 hrs by MTT assay, IC50=16.35μM	25906385
MCF7	Cytotoxicity assay		72 hrs	Cytotoxicity against human MCF7 cells assessed as cell growth inhibition after 72 hrs by MTT assay, IC50=20.9μM	25906385
2008	Cytotoxicity assay		72 hrs	Cytotoxicity against human 2008 cells assessed as cell growth inhibition after 72 hrs by MTT assay, IC50=21.18μM	25906385
A431	Cytotoxicity assay		72 hrs	Cytotoxicity against human A431 cells assessed as cell growth inhibition after 72 hrs by MTT assay, IC50=23.04μM	25906385
BxPC3	Cytotoxicity assay		72 hrs	Cytotoxicity against human BxPC3 cells assessed as cell growth inhibition after 72 hrs by MTT assay, IC50=24.12μM	25906385
A375	Cytotoxicity assay		72 hrs	Cytotoxicity against human A375 cells assessed as cell growth inhibition after 72 hrs by MTT assay, IC50=25.13μM	25906385
A431	Cytotoxicity assay		72 hrs	Cytotoxicity against cisplatin-resistant human A431 cells assessed as cell growth inhibition after 72 hrs by MTT assay, IC50=34.37μM	25906385
C13	Cytotoxicity assay		72 hrs	Cytotoxicity against human C13 cells assessed as cell growth inhibition after 72 hrs by MTT assay, IC50=37.62μM	25906385
B16-4A5	Function assay		72 hrs	Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs by microplate reader analysis, IC50=15μM	25987378
RAW264.7	Anti-inflammatory assay		24 hrs	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production incubated for 24 hrs by Griess reagent based assay, IC50=1.8μM	26143931
BV2	Anti-inflammatory assay		24 hrs	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-stimulated nitric oxide production after 24 hrs by Griess assay, IC50=17.4μM	26327273
LNCAP	Cytotoxicity assay		3 days	Cytotoxicity against human androgen-dependent LNCAP cells assessed as inhibition of cell viability after 3 days by WST-1 cell proliferation assay, IC50=45.5μM	26341135
BV2	Antineuroinflammatory assay		24 hrs	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-stimulated NO production after 24 hrs by Griess assay, EC50=11μM	26473791
BV2	Function assay		24 hrs	Inhibition of LPS-induced nitric oxide production in human BV2 cells after 24 hrs by Griess assay, IC50=8.7μM	26696523
HepG2	Antioxidant assay		2.5 hrs	Antioxidant activity in human HepG2 cells assessed as decrease in fluorescence level after 2.5 hrs measured every 5 mins for 1 hr by DCFH-DA staining based fluorescence assay, EC50=4.63μM	26974372
RF/6A	Antimigratory assay		24 hrs	Antimigratory activity in rhesus monkey RF/6A cells measured after 24 hrs by wound assay, IC50=40μM	27015547
RAW264.7	Function assay		24 hrs	Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 24 hrs by Griess assay, IC50=15.9μM	27177824
BV2	Antiinflammatory assay		24 hrs	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs measured after 15 mins by Griess assay, IC50=15.13μM	27335254
LNCAP	Antiproliferative assay		3 days	Antiproliferative activity against human LNCAP cells after 3 days by WST1 assay, IC50=45.5μM	27476422
BV2	Antineuroinflammatory assay		24 hrs	Antineuroinflammatory activity in human BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction, IC50=39.46μM	27623545
N9	Antineuroinflammatory assay		24 hrs	Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay, IC50=10.22μM	28073678
Escherichia coli competent cells	Function assay		overnight	Inhibition of amyloid beta (1 to 42) aggregation in Escherichia coli competent cells BL21 (DE3) after overnight incubation by Thioflavin-S steady-state fluorescence assay, IC50=15.3μM	28128562
BV2	Antiinflammatory assay		24 hrs	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay, IC50=19.11μM	28165740
RAW264.7	Function assay		1 hr	Inhibition of LPS-induced NO production in mouse RAW264.7 cells preincubated for 1 hr followed by LPS stimulation measured after 24 hrs by Griess assay, IC50=4.41μM	28427811
human red blood cells	Antiplasmodial assay		48 hrs	Antiplasmodial activity against asexual erythrocyte stage of chloroquine-sensitive Plasmodium falciparum 3D7 infected in human red blood cells after 48 hrs by lactate dehydrogenase assay, IC50=17.54μM	28557449
T47D	Cytotoxicity assay		48 hrs	Cytotoxicity against human T47D cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=11μM	28654265
NCI-H460	Cytotoxicity assay		48 hrs	Cytotoxicity against human NCI-H460 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=12μM	28654265
SK-MEL-2	Cytotoxicity assay		48 hrs	Cytotoxicity against human SK-MEL-2 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=12μM	28654265
LN229	Cytotoxicity assay		48 hrs	Cytotoxicity against human LN229 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=12μM	28654265
A549	Cytotoxicity assay		48 hrs	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=13μM	28654265
MML1	Cytotoxicity assay		48 hrs	Cytotoxicity against human MML1 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=15μM	28654265
BxPC3	Cytotoxicity assay		48 hrs	Cytotoxicity against human BxPC3 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=16μM	28654265
HT-29	Cytotoxicity assay		48 hrs	Cytotoxicity against human HT-29 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=18μM	28654265
COLO201	Cytotoxicity assay		48 hrs	Cytotoxicity against human COLO201 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=19μM	28654265
MDA-MB-231	Cytotoxicity assay		48 hrs	Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=20μM	28654265
U87	Cytotoxicity assay		48 hrs	Cytotoxicity against human U87 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=23μM	28654265
LNCAP	Antiproliferative assay		3 days	Antiproliferative activity against human LNCAP cells after 3 days by WST-1 assay, IC50=45.46μM	28760528
BV2	Antiinflammatory assay		24 hrs	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by griess assay, IC50=7.2μM	29303577
K562	Cytotoxicity assay		5 days	Cytotoxicity against human K562 cells assessed as inhibition of cell growth after 5 days by MTT assay, IC50=2.5μM	29553260
HepG2	Antioxidant assay		60 mins	Antioxidant activity in human HepG2 cells assessed as inhibition of ABAP-induced peroxyl radical generation incubated for 60 mins measured for 60 mins by DCFH-DA probe based fluorescence assay, IC50=12μM	29553260
BV2	Antiinflammatory assay		24 hrs	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay, IC50=4.3μM	30119995
BV2	Function assay		24 hrs	Inhibition of LPS-induced nitric oxide production in mouse BV2 cells after 24 hrs by Griess assay, IC50=2.4μM	30132670
MCF10A	Cytotoxicity assay		3 days	Cytotoxicity against human MCF10A cells assessed as decrease in cell viability after 3 days by WST-1 assay, IC50=11.97μM	30165345
PWR-1E	Cytotoxicity assay		3 days	Cytotoxicity against human PWR-1E cells assessed as decrease in cell viability after 3 days by WST-1 assay, IC50=15.08μM	30165345
LNCAP	Antiproliferative assay		3 days	Antiproliferative activity against human LNCAP cells after 3 days by WST-1 assay, IC50=45.5μM	30165345
Jurkat	Function assay		24 hrs	Inhibition of chymotrypsin-like activity of human 26S proteasome in human Jurkat cells assessed as decrease in AMC hydrolysis using Z-Gly-Gly-Leu-AMC as substrate after 24 hrs by fluorescence based method, IC50=2μM	30776692
Jurkat	Function assay		2 hrs	Inhibition of chymotrypsin-like activity of purified human 20S proteasome expressed in human Jurkat cells assessed as decrease in AMC hydrolysis using Suc-Leu-Leu-Val-Tyr-AMC as substrate incubated for 2 hrs by fluorescence based method, IC50=3.5μM	30776692
HEK293	Function assay		30 mins	Inhibition of human TRPC5 expressed in HEK293 cells assessed as reduction in gadolinium-induced calcium entry after 30 mins by fluo-4 dye based fluorescence assay, IC50=6.5μM	30943030
EPG85-257RDB	Function assay		72 hrs	Downregulation of ABCB1 gene expression in human EPG85-257RDB cells after 72 hrs by RT-PCR analysis, IC50=12μM	31103904
PANC1	Anti-austerity assay		24 hrs	Anti-austerity activity against nutrient-deprived human PANC1 cells assessed as cell death incubated for 24 hrs by WST-8 assay, PC50=21.8μM	31682126
BM	Antioxidant assay			Antioxidant activity in BALB/c mouse BM cells assessed as inhibition of ROS production, IC25=3.5μM	17166721
Jurkat T	Function assay			Activity against hydrogen peroxide induced DNA damage in Jurkat T cells, IC50=5μM	16392814
HT22	Function assay			Inhibition of glutamate-induced oxytosis of mouse hippocampal HT22 cells, EC50=2.98μM	16392814
RAW264.7	Function assay			Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells, IC50=26.8μM	16378364
RAW264.7	Function assay			Inhibition of LPS and INF-gamma-induced NO production in mouse RAW264.7 cells, IC50=26.8μM	15043424
HeLa	Function assay			TP_TRANSPORTER: inhibition of LTC4 uptake in membrane vesicle from MRP1-expressing HeLa cells, Ki=8.1μM	11306701
HEK293	Function assay			Displacement of [125I]AB-MECA binding to human Adenosine A3 receptor expressed in HEK293 cells, Ki=30μM	8576921
RBL-1	Function assay			In vitro inhibition against 5-lipoxygenase in RBL-1 cells was determined, IC50=0.2μM	8254620
WiDr human colon cells	Cytotoxicity assay			Cytotoxic effect on WiDr human colon cells, IC50=40.2μM	8201603
3T3	Cytotoxicity assay			Cytotoxic effect on 3T3 cells, IC50=39.5μM	8201603
ANN-1	Cytotoxicity assay			Cytotoxic effect on v-abl transformed murine ANN-1 cells, IC50=32.5μM	8201603
MCF-7	Cytotoxicity assay			Cytotoxic effect on MCF-7 human breast carcinoma cells, IC50=24μM	8201603
RBI-1	Function assay			In vitro inhibition of 5-lipoxygenase (5-lo) from the 20000 g supernatant of RBI-1 cells, IC50=0.3μM	2066989
Walker 256	Antitumor assay			In vivo antitumor activity against rat Walker 256 cells	469554
Sf21	Function assay			Inhibition of human recombinant COX2 expressed in insect Sf21 cells by EIA assay, IC50=28.6μM	17378609
LNCaP	Function assay			Decrease in androgen receptor protein expression in LNCaP cells by Western blotting, EC50=25μM	17383188
RAW264.7	Function assay			Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells, IC50=26.8μM	18027905
BV2	Function assay			Inhibition of LPS-induced NO production in mouse BV2 cells, IC50=29.6μM	18178435
HeLa	Function assay			Inhibition of MRP1 transfected in human HeLa cells assessed as inhibition of [3H]LTC4 transport by rapid filtration assay, Ki=2.4μM	19725578
MDCK	Antiviral assay			Antiviral activity against influenza A virus H1N1 A/PR/8/34 infected in MDCK cells assessed as reduction in virus-induced cytopathic effect, EC50=43.1μM	19729316
Sf9	Function assay			Inhibition of rat ecto-5'-nucleotidase expressed in Sf9 cells by capillary electrophoresis method, Ki=0.0453μM	20146483
HUVEC	Cytoprotective assay			Cytoprotective activity against H2O2-induced cell injury in HUVEC cells, EC50=18μM	20621727
HEK293 FS	Function assay			Inhibition of NOX4 expressed in HEK293 FS cells assessed as H2O2 production by H2O2/Tyr/LPO assay, IC50=0.68μM	20731357
3T3L1	Function assay			Inhibition of AMPK-mediated adipocyte differentiation in mouse 3T3L1 cells	20822902
3T3L1	Function assay			Inhibition of PPAR-gamma-mediated adipocyte differentiation in mouse 3T3L1 cells	20822902
MDCK	Function assay			Inhibition of BCRP expressed in MDCK cells using Hoechst 33342 staining, IC50=6.9μM	21354800
MCF-7 MX	Function assay			Inhibition of BCRP expressed in MCF-7 MX cells using Hoechst 33342 staining, IC50=7.6μM	21354800
HepG2	Cytotoxicity assay			Cytotoxicity against human HepG2 cells by MTT assay, IC50=30.9μM	21726077
human mast cells	Function assay			Inhibition of SYK in human mast cells assessed as reduction in mast cell degranulation, EC50=35μM	22257213
22Rv1	Antiproliferative assay			Antiproliferative activity against human 22Rv1 cells incubated up to 144 hrs by Coulter particle count and size analyzer, IC50=15.5μM	22789812
BV2	Antiinflammatory assay			Antiinflammatory activity in human BV2 cells assessed as inhibition of LPS-induced nitric oxide production by Griess assay, IC50=10μM	24707938
HeLa	Anticancer assay			Anticancer activity against human HeLa cells by HTS assay, IC50=3.56μM	25139569
NCI-H1792	Anticancer assay			Anticancer activity against human NCI-H1792 cells by HTS assay, IC50=3.85μM	25139569
LOXIMVI	Anticancer assay			Anticancer activity against human LOXIMVI cells by HTS assay, IC50=4.65μM	25139569
NCI-H522	Anticancer assay			Anticancer activity against human NCI-H522 cells by HTS assay, IC50=5.77μM	25139569
NCI-H157	Anticancer assay			Anticancer activity against human NCI-H157 cells by HTS assay, IC50=6μM	25139569
A549	Anticancer assay			Anticancer activity against human A549 cells by HTS assay, IC50=6.2μM	25139569
HOP62	Anticancer assay			Anticancer activity against human HOP62 cells by HTS assay, IC50=7.52μM	25139569
H460	Anticancer assay			Anticancer activity against human H460 cells by HTS assay, IC50=9.62μM	25139569
NCI-H1944	Anticancer assay			Anticancer activity against human NCI-H1944 cells by HTS assay, IC50=10.18μM	25139569
M14	Anticancer assay			Anticancer activity against human M14 cells by HTS assay, IC50=12.77μM	25139569
H1299	Anticancer assay			Anticancer activity against human H1299 cells by HTS assay, IC50=13.6μM	25139569
SKBR	Anticancer assay			Anticancer activity against human SKBR cells by HTS assay, IC50=16.71μM	25139569
H266	Anticancer assay			Anticancer activity against human H266 cells by HTS assay, IC50=16.87μM	25139569
M4E	Anticancer assay			Anticancer activity against human M4E cells by HTS assay, IC50=21.71μM	25139569
Calu1	Anticancer assay			Anticancer activity against human Calu1 cells by HTS assay, IC50=23.58μM	25139569
RAW264.7	Anti-inflammatory assay			Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production by Griess assay, IC50=15μM	26928174
BV2	Antiinflammatory assay			Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-stimulated nitric oxide production by Griess reagent based spectrophotometric analysis, IC50=16.3μM	29122483
HEK293	Function assay			Inhibition of human CYP1B1 expressed in HEK293 cells by fluorescence assay, IC50=2.2μM	29150398
MDA-MB-468	Function assay			Inhibition of human CYP1B1 expressed in MDA-MB-468 cells assessed as cell toxicity, IC50=2.2μM	29150398
HEK293	Function assay			Inhibition of human CYP1A expressed in HEK293 cells by fluorescence assay, IC50=9.9μM	29150398
HEK293	Function assay			Inhibition of human CYP1A1 transfected in HEK293 cells assessed as cell toxicity, IC50=9.9μM	29150398
DAOY	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for DAOY cells	29435139
A673	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells	29435139
SK-N-MC	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells	29435139
NB-EBc1	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells	29435139
SK-N-SH	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-SH cells	29435139
NB1643	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB1643 cells	29435139
LAN-5	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for LAN-5 cells	29435139
他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

生物活性

製品説明

Targets

Sirtuin ^[4]	Src ^[1]	PKC ^[1]	PI3Kγ ^[1] (Cell-free assay)	PI3Kδ ^[1] (Cell-free assay)	もっとクリックする
			2.4 μM	3.0 μM	もっとクリックする

In Vitro
In vitro	Quercetin is a type of plant-based chemical, or phytochemical, known as a flavonol and a plant-derived flavonoid found in fruits, vegetables, leaves and grains. It also may be used as an ingredient in supplements, beverages or foods. In several studies, it may have anti-inflammatory and antioxidant properties, and it is being investigated for a wide range of potential health benefits. Quercetin (Sophoretin) is a PI3K inhibitor with IC50 of 2.4 – 5.4 μM. It strongly abrogated PI3K and Src kinases, mildly inhibited Akt1/2, and slightly affected PKC, p38 and ERK1/2. ^[1] Quercetin inhibits TNF-induced LDH% release, EC-dependent neutrophils adhesion to bovine pulmonary artery endothelial cells (BPAEC), and BPAEC DNA synthesis and proliferation. ^[2]
細胞実験	細胞株	Sertoli cells
	濃度	0.1 μM
	反応時間	24 h
	実験の流れ	Cells were treated with various concentrations of drug for 24 h.
実験結果図	Methods	Biomarkers	結果図	PMID
	Western blot	Bcl-2 / Bax / Bcl-xl p-p53 / p53 / p21 p-JAK1 / p-STAT3 / STAT3 / VEGF Her-2 / p-PI3K / p-Akt / Akt		27175602
	Growth inhibition assay	Cell viability Cell viability		27175602

In Vivo
In Vivo	Combination of Quercetin (75 mg/kg) and 2-Methoxyestradiol enhances inhibition of human prostate cancer LNCaP and PC-3 cells xenograft tumor growth.^[3] LD50: >3000mg/kg (i.g.) ^[4]

In Vivo

Combination of Quercetin (75 mg/kg) and 2-Methoxyestradiol enhances inhibition of human prostate cancer LNCaP and PC-3 cells xenograft tumor growth.^[3]

LD50: >3000mg/kg (i.g.) ^[4]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
臨床試験 (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT06003270	Not yet recruiting	Chronic Obstructive Pulmonary Disease	Temple University\|National Institutes of Health (NIH)\|Quercegen Pharmaceuticals\|National Center for Complementary and Integrative Health (NCCIH)	September 2023	Phase 2
NCT04907253	Active not recruiting	Coronary Artery Disease	Montreal Heart Institute	June 4 2021	Phase 2
NCT04474626	Withdrawn	Sickle Cell Disease\|Sickle Cell-Beta0-Thalassemia	Jeffrey Zwicker MD\|Quercegen Pharmaceuticals\|Dana-Farber Cancer Institute	December 2020	Phase 2
NCT04063124	Completed	Alzheimer Disease	The University of Texas Health Science Center at San Antonio\|Mayo Clinic	February 14 2020	Phase 1\|Phase 2
NCT03989271	Recruiting	Chronic Obstructive Pulmonary Disease	Temple University\|National Institutes of Health (NIH)\|Quercegen Pharmaceuticals\|National Center for Complementary and Integrative Health (NCCIH)	October 1 2019	Phase 1\|Phase 2

参考
[1] Navarro-Nunez L, et al. Fitoterapia, 2010, 81(2):75-80. [2] Yu XB, et al. Yao Xue Xue Bao, 1996, 31(3), 176-181. [3] Yang F, et al. PLoS One. 2015, 10(5), e0128277. [5] Liu DL, et al. Int J Mol Sci. 2022 Sep 22;23(19):11163. [6] Sun J, et al. Nat Commun. 2022 Nov 17;13(1):7028.

参考

化学情報

分子量	302.24	化学式	C₁₅H₁₀O₇
CAS No.	117-39-5	SDF	Download Quercetin SDFをダウンロードする
Smiles	C1=CC(=C(C=C1C2=C(C(=O)C3=C(C=C(C=C3O2)O)O)O)O)O
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1个月-20°Cin solvent

In vitro
Batch:

DMSO : 60 mg/mL ( (198.51 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : Insoluble

Ethanol : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):