BAY 11-7082

BAY 11-7082 (BAY 11-7821) is a NF-κB inhibitor, inhibits TNFα-induced IκBα phosphorylation with IC50 of 10 μM in tumor cells. BAY 11-7082 inhibits ubiquitin-specific protease USP7 and USP21 with IC50 of 0.19 μM and 0.96 μM, respectively. BAY 11-7082 induces apoptosis and S phase arrest in gastric cancer cells.

CAS No. 19542-67-7

サイズ	価格(税別)	在庫状況
10mg	JPY 22000	国内在庫あり
50mg	JPY 55500	国内在庫あり
1g	JPY 298500	国内在庫なし(納期7~10日)

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

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製品安全説明書

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BAY 11-7082関連製品

関連ターゲット	IκB IKK	もっと見る
関連製品	IKK-16 TPCA-1 Bay 11-7085 BMS-345541 IMD 0354 Bardoxolone Methyl MRT67307 HCl SC-514 LY2409881 TBK1/IKKε-IN-5 PS-1145 Dehydrocostus Lactone Wedelolactone WS6 Mesalazine (5-ASA) BAY-985 AZD3264 WS3 TBK1/IKKε-IN-2 Rosmarinic acid	もっと見る
関連化合物ライブラリー	FDA-approved Drug Library Immunology/Inflammation Compound Library Small Molecule Immuno-Oncology Compound Library NF-κB Signaling Compound Library FDA-approved Anticancer Drug Library	もっと見る

シグナル伝達経路

IκB/IKKシグナル伝達経路

IκB/IKK阻害剤の選択性比較

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	活性情報	PMID
SP6.5	Growth Inhibition Assay	2.5-20 μM	24 h	IC50=5 μM, exhibits strong anti-proliferative effects in a dose-dependent manner	23443086
OM431	Function Assay	5 μM	2 h	decreases translocation of p65 in the nucleus	23443086
OCM1	Function Assay	5 μM	2 h	decreases translocation of p65 in the nucleus	23443086
VUP	Function Assay	5 μM	2 h	decreases translocation of p65 in the nucleus	23443086
SP6.5	Function Assay	5 μM	2 h	decreases translocation of p65 in the nucleus	23443086
BAFs	Function Assay	0.5/1 μM	24 h	inhibits TNFα/DEX induced CYP19A1 transcripts	23485457
KMS-12-BM	Apoptosis Assay	30 µM	3 h	induces MM cell death involves necrosis	23527154
MM.1S	Apoptosis Assay	30 µM	3 h	induces MM cell death involves necrosis	23527154
HT29	Function Assay	10/30/100 μM	1 h	inhibits TNF-induced phosphorylation and degradation of IκBα	23527154
HT29	Function Assay	10/30/100 μM	1 h	inhibites both TWEAK-induced p100 processing	23527154
HUVEC	Function Assay	10 μM	0.5 h	counteractes the loss of Tie2 mRNA	23563632
RAW 264	Function Assay	0.2-5 µM	30/60/90 min	inhibits the phosphatase activity of PTP1B	23578302
AM	Growth Inhibition Assay	5 µM	4/8 d	reduces the viability of intracellular MTB	23634218
MDM	Growth Inhibition Assay	5 µM	4/8 d	reduces the viability of intracellular MTB	23634218
THP-1	Growth Inhibition Assay	5 µM	4/8 d	reduces the viability of intracellular MTB	23634218
THP-1	Function Assay	5 µM	1 h	inhibits MTB-induced NFκB activation	23634218
HepG2	Function Assay	0.3/1/3 μM	48 h	reduces IL6-induced PON1 expression	23791833
HBE	Function Assay	10μM	3h	abolishes the increases of IL-6 expression induced by CSE	23824089
HGC-27	Apoptosis Assay	7.5/15/30 μM	6 h	induces apoptosis in a time- and dose-dependent manner	23846545
MGC80-3	Function Assay	7.5/15/30 μM	6 h	induces the dephosphorylation and up-regulation of IκBα	23846545
HGC-27	Function Assay	7.5/15/30 μM	6 h	induces the dephosphorylation and up-regulation of IκBα	23846545
MGC80-3	Growth Inhibition Assay	10/20/30 μM	6/24 h	suppresses cell viability significantly	23846545
AGS	Growth Inhibition Assay	10/20/30 μM	6/24 h	suppresses cell viability significantly	23846545
NCI-N87	Growth Inhibition Assay	10/20/30 μM	6/24 h	suppresses cell viability significantly	23846545
A549	Apoptosis Assay	1 μM	48 h	reduces the cell death induced by combined stimulation	23900080
A549	Function Assay	1 μM	48 h	enhances the up-regulation of IκB and subsequent decrease in Bax expression induced by combined stimulation	23900080
THP-1	Function Assay	10 μM	1 h	abolishes the effect of rHSP27 on SR-A mRNA	23939398
PC-3	Function Assay	2.5/5/10 μM	0.5 h	blocks IGF-II-induced STS mRNA expression	24055520
iNKT	Function Assay	10/100 μM	0.5 h	inhibits the induction of A2AR mRNA and other factor	24124453
IVD	Function Assay	10 μM	3 d	abrogates TNF-α–induced up-regulation of ADAMTS-4 and ADAMTS-5	24176808
IVD	Function Assay	10 μM	3 d	reverses TNF-α–mediated suppression of the disc matrix macromolecules aggrecan and collagen II	24176808
A549RT-eto	Function Assay	10 μM	24 h	decreases the expression levels of NF-κB and P-gp	24220725
A549RT-eto	Apoptosis Assay	10 μM	24 h	accelerates F14 extract-mediated apoptosis combined treatment with F14	24220725
WPs	Function Assay	25 μM	5 min	suppresses ATP and vWF secretion	24331207
DSCs	Function Assay	2.5 μM	0.5 h	reverses the enhancement of CCL2/CCR2 expression of DSCs induced by IL-33	24344240
OVCXCR2	Function Assay	2 µM	48 h	blocks the CXCL1-induced cell invasion	24376747
SKCXCR2	Function Assay	2 µM	48 h	blocks the CXCL1-induced cell invasion	24376747
SKCXCR2	Growth Inhibition Assay	2 µM	48 h	decreases cell proliferation significantly	24376747
THP-1	Function Assay	0.1/1 μM	0.5 h	abrogates TNF-α secretion as well as the increased secretion of IL-6 and IL-1β	24378536
A549RT-eto	Apoptosis Assay	10 μM	24 h	accelerates FERO-mediated apoptosis	24535083
HUVEC	Function Assay	20 µM	0.5 h	prevents the induction of EAM expression	24551209
A549	Function Assay	10 μM	1 h	inhibits the increase of phospho-IκBα in PA103-infected cultures	24612488
HeLa	Function Assay	5 μM	24 h	reduces the activity of TNF-α promoter	24657783
HUVECs	Function Assay	3-30 μM	1 h	reduces the expression of miR-146a in a dose-dependent manner	24863965
macrophages	Function Assay	5 µM	3 h	partially blocks YPFS-induced expression of iNOS and COX-2	24967898
RAW 264.7	Function Assay	5 μM	1 h	inhibits TNF-α and IL-12 p40 production	25019567
A549	Function Assay	10 µM	12 h	suppresses Dvl-3 induced activation of p65	25156800
HMECs	Function Assay	5 μM	2 h	abolishes TNF-α-induced VCAM-1 expression	25193116
HCT116	Function Assay	5 μM	2 h	attenuates silymarin-induced downregulation of cyclin D1	25479723
ARPE-19	Function Assay	1 μM	0.5 h	suppresses TG-induced IL-8 promoter activation	25593029
SiHa	Function Assay	10 μM	1.5 h	abolishes BPA induced up regulation of FN and MMP-9	25797437
HeLa	Function Assay	10 μM	1.5 h	abolishes BPA induced up regulation of FN and MMP-9	25797437
VUP	Growth Inhibition Assay	2.5-20 μM	24 h	IC50=5 μM, exhibits strong anti-proliferative effects in a dose-dependent manner	23443086
OCM1	Growth Inhibition Assay	2.5-20 μM	24 h	IC50=5 μM, exhibits strong anti-proliferative effects in a dose-dependent manner	23443086
OM431	Growth Inhibition Assay	2.5-20 μM	24 h	IC50=5 μM, exhibits strong anti-proliferative effects in a dose-dependent manner	23443086
SP6.5	Apoptosis Assay	5 μM	24 h	induces apoptosis	23443086
VUP	Apoptosis Assay	5 μM	24 h	induces apoptosis	23443086
OCM1	Apoptosis Assay	5 μM	24 h	induces apoptosis	23443086
OM431	Apoptosis Assay	5 μM	24 h	induces apoptosis	23443086
SP6.5	Function Assay	5 μM	12 h	reduces the migration	23443086
VUP	Function Assay	5 μM	12 h	reduces the migration	23443086
OCM1	Function Assay	5 μM	12 h	reduces the migration	23443086
OM431	Function Assay	5 μM	12 h	reduces the migration	23443086
HBL-1	Growth Inhibition Assay	3 μM	24/48/72 h	slows cell growth modestly	23441730
RAW 264.7	Function Assay	2-15 μM	1 h	suppresses the activation of IKK family members	23441730
IL-1R	Function Assay	2-15 μM	1 h	suppresses the activation of IKK family members	23441730
RAW 264.7	Function Assay	15 μM	1 h	suppresses the activation of and JNK	23441730
IL-1R	Function Assay	15 μM	1 h	suppresses the activation of and JNK	23441730
U2OS	Function Assay	15 μM	1 h	prevents the LPS- or IL-1-stimulated formation of K63-pUb chains	23441730
MT‐1	Function Assay	8 µm	3 h	decreases the levels of p‐STAT3 and p‐4EBP1	23278479
MT‐2	Function Assay	8 µm	3 h	decreases the levels of p‐STAT3 and p‐4EBP1	23278479
MT‐1	Function Assay	8 µm	3 h	decreases the levels of the p65 subunit of NF‐κB	23278479
MT‐2	Function Assay	8 µm	3 h	decreases the levels of the p65 subunit of NF‐κB	23278479
MCF-7	Function Assay	2.5-15 μM	0.5 h	causes the gradual loss of cell adhesion	23093227
HaCaT	Function Assay	5.0 μM	1 h	attenuates the TCOH-induced production of IL-6	23041168
OA chondrocytes	Function Assay	10 μM	1 h	blocks the AGE-BSA-induced gene/protein expression of GRP78 or COX-2 (p<0.05)	22982228
RAW264.7	Function Assay	15 μM	15-120 min	blocks the production of NO, PGE2, and TNF-α	22745523
RAW264.7	Growth Inhibition Assay	5-30 μM	24 h	inhibits cell growth in a dose-dependent manner	22745523
HBL6	Apoptosis Assay	0.5/5/25 μM	6/24 h	decreases cell viability and leeads to apoptosis in a dose-dependent manner	22074820
HT29	Function Assay	1-10 μM	10 h	increases HO-1 mRNA and protein expression	21620964
Ca9–22	Apoptosis Assay	10 μM	1 h	completely inhibits ALA-PDT-induced apoptosis	21138480
Ca9–22	Function Assay	10 μM	1 h	completely abrogates the ALA-PDT-induced JNK activation	21138480
A-549	Growth Inhibition Assay	10 μM	24/48 h	inhibits cell growth in a time-dependent manner	20866043
AP	Function Assay	5/10 μM	48 h	downregulates the BAD protein level a dose-dependent manner	20596645
AQ1	Function Assay	5/10 μM	48 h	downregulates the BAD protein level a dose-dependent manner	20596645
AP	Function Assay	20 μM	4/8 h	downregulates the BAD protein level a time-dependent manner	20596645
AQ1	Function Assay	20 μM	4/8 h	downregulates the BAD protein level a time-dependent manner	20596645
THP-1	Function Assay	5 μM	0.5 h	attenuates the LPS-induced p-IκBα protein by 72%	20309718
K562	Growth Inhibition Assay	2-30 μM	24 h	IC50=8 μM,inhibits cell growth in a dose-dependent manner	19646807
Jurket	Growth Inhibition Assay	2-30 μM	24 h	IC50=7.1 μM, inhibits cell growth in a dose-dependent manner	19646807
U937	Growth Inhibition Assay	2-30 μM	24 h	IC50=10.5 μM, inhibits cell growth in a dose-dependent manner	19646807
PBMC	Growth Inhibition Assay	2-30 μM	24 h	IC50=40.2 μM, inhibits cell growth in a dose-dependent manner	19646807
K562	Apoptosis Assay	2-20 μM	24 h	induces a dose-dependent apoptosis	19646807
HEK293	Function assay	20 uM	24 hrs	Inhibition of TNF-alpha stimulated NFkappaB transactivation in HEK293 cells at 20 uM measured after 24 hrs by dual luciferase reporter gene assay	27736063
RAW264.7	Function assay	20 uM	1 hr	Inhibition of LPS-induced NFkB activation in mouse RAW264.7 cells assessed as reduction in nuclear translocation of p65 at 20 uM preincubated for 1 hr followed by LPS stimulation measured after 3 hrs by Western blot method	28667873
RAW264.7	Function assay	20 uM	6 hrs	Inhibition of LPS-induced NF-kappaB activation in mouse RAW264.7 cells at 20 uM treated 30 mins before LPS challenge measured after 6 hrs by luciferase reporter gene assay	24315191
RAW264.7	Antinflammatory assay	20 uM	18 hrs	Antinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production at 20 uM treated 30 mins before LPS challenge measured after 18 hrs by Griess assay	24315191
THP1	Antinflammatory assay	5 uM	24 hrs	Antiinflammatory activity in human THP1 cells assessed as inhibition of TPA/ionomycin-induced extracellular IL-1beta level at 5 uM incubated 1 hr prior to TPA/ionomycin challenge measured after 24 hrs by ELISA	24400858
THP1	Antinflammatory assay	5 uM	24 hrs	Antiinflammatory activity in human THP1 cells assessed as inhibition of TPA/ionomycin-induced extracellular TNF-alpha production at 5 uM incubated 1 hr prior to TPA/ionomycin challenge measured after 24 hrs by ELISA	24400858
THP1	Antinflammatory assay	5 uM	24 hrs	Antiinflammatory activity in human THP1 cells assessed as inhibition of TPA/ionomycin-induced intracellular proIL-1beta level at 5 uM incubated 1 hr prior to TPA/ionomycin challenge measured after 24 hrs by ELISA	24400858
THP1	Antinflammatory assay	5 uM	24 hrs	Antiinflammatory activity in human THP1 cells assessed as inhibition of TPA/ionomycin-induced intracellular IL-1beta level at 5 uM incubated 1 hr prior to TPA/ionomycin challenge measured after 24 hrs by ELISA	24400858
RAW264.7	Function assay	0.3 ug/ml	12 hrs	Inhibition of LPS-induced NF-kB p65 phosphorylation in mouse RAW264.7 cells at 0.3 ug/ml preincubated for 12 hrs followed by LPS stimulation for 3 hrs by Western blot method	28284806
RAW264.7	Function assay	0.3 ug/ml	12 hrs	Inhibition of LPS-induced NF-kB p65 activation in mouse RAW264.7 cells at 0.3 ug/ml preincubated for 12 hrs followed by LPS stimulation for 3 hrs by DAPI staining based inverted fluorescence microscopic method	28284806
RAW264.7	Function assay	0.3 ug/ml	12 hrs	Inhibition of NF-kB p65 in mouse RAW264.7 cells assessed as reduction in LPS-induced iNOS expression at 0.3 ug/ml preincubated for 12 hrs followed by LPS stimulation for 3 hrs by Western blot method	28284806
RAW264.7	Function assay	0.3 ug/ml	12 hrs	Inhibition of NF-kB p65 in mouse RAW264.7 cells assessed as reduction in LPS-induced COX2 expression at 0.3 ug/ml preincubated for 12 hrs followed by LPS stimulation for 3 hrs by Western blot method	28284806
HEK293	Function assay	20 uM	24 hrs	Inhibition of TNFalpha-induced NFkappaB activation in HEK293 cells at 20 uM after 24 hrs by dual luciferase reporter gene assay	28873303
RAW264.7	Function assay	20 uM	2 hrs	Inhibition of NFkappaB nuclear translocation in LPS-stimulated mouse RAW264.7 cells at 20 uM pretreated for 2 hrs followed by LPS-induction by DAPI-staining based immunofluorescence microscopic method	29759725
BGC823	Function assay	5 uM	12 hrs	Inhibition of colony formation in human BGC823 cells at 5 uM treated for 12 hrs followed by incubation in drug free medium for 14 days by crystal violet staining based assay	28881286
SGC7901	Function assay	5 uM	12 hrs	Inhibition of colony formation in human SGC7901 cells at 5 uM treated for 12 hrs followed by incubation in drug free medium for 14 days by crystal violet staining based assay	28881286
RAW264.7	Function assay	10 uM	2 hrs	Inhibition of LPS-induced IL-6 mRNA expression in mouse RAW264.7 cells at 10 uM pre-incubated for 2 hrs before LPS stimulation for 24 hrs by qRT-PCR method	27038497
RAW264.7	Function assay	10 uM	2 hrs	Inhibition of LPS-induced IL-1beta mRNA expression in mouse RAW264.7 cells at 10 uM pre-incubated for 2 hrs before LPS stimulation for 24 hrs by qRT-PCR method	27038497
RAW264.7	Function assay	10 uM	2 hrs	Inhibition of LPS-induced iNOS mRNA expression in mouse RAW264.7 cells at 10 uM pre-incubated for 2 hrs before LPS stimulation for 24 hrs by qRT-PCR method	27038497
FaDu	Function Assay		2 h	inhibits p65 expression and blocks TNFα-induced TWIST expression	24220622
A549	Function Assay		1 h	inhibits LTA-induced SP-A mRNA production significantly	23031213
THP1	Cytotoxicity assay		72 hrs	Cytotoxicity against human THP1 cells assessed as reduction in cell viability after 72 hrs by MTT assay, TC50 = 1.5 μM.	28410442
RAW264.7	Function assay		6 hrs	Inhibition of LPS-induced NF-kappaB activation in mouse RAW264.7 cells treated 30 mins before LPS challenge measured after 6 hrs by luciferase reporter gene assay, IC50 = 1.72 μM.	24315191
HEK293	Function assay		6 hrs	Inhibition of TNF-alpha-induced NF-kappaB activity in HEK293 cells after 6 hrs by luciferase reporter gene assay, IC50 = 2 μM.	24533857
HEK293	Function assay		6 hrs	Inhibition of TNF-alpha-induced NF-kappaB activity in HEK293 cells after 6 hrs by luciferase reporter gene assay, IC50 = 2 μM.	24992702
HEK293	Function assay		6 hrs	Inhibition of TNFalpha-induced NF-kappaB activity (unknown origin) transfected in HEK293 cells after 6 hrs by luciferase reporter gene assay, IC50 = 2 μM.	26343828
HEK293	Function assay		6 hrs	Inhibition of TNFalpha-induced NF-kappaB activity expressed in human HEK 293 cells after 6 hrs by luciferase reporter gene assay, IC50 = 2 μM.	22850207
HEK293	Function assay		6 hrs	Inhibition of TNFalpha-induced human NFkappaB activity in HEK293 cells incubated for 6 hrs followed by compound wash out measured after 5 mins by by luciferase assay, IC50 = 2.01 μM.	22712432
HEK293	Function assay		6 hrs	Inhibition of TNFalpha-induced NFkappaB (unknown origin) activation expressed in HEK293 cells after 6 hrs by luciferase reporter gene assay, IC50 = 5 μM.	23316950
HEK293	Cytotoxicity assay			Cytotoxicity against HEK293 cells, IC50 = 3.8 μM.	24533857
HEK293	Function assay			Effect on Cdc2 expressed in HEK293 cells assessed as effect on Cdc2:Cdc25C interaction complexes in presence of camptothecin by EYFP and/or YFP Venus fragment based reporter gene assay	16680159
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生物活性

製品説明

BAY 11-7082 (BAY 11-7821) is a NF-κB inhibitor, inhibits TNFα-induced IκBα phosphorylation with IC50 of 10 μM in tumor cells. BAY 11-7082 inhibits ubiquitin-specific protease USP7 and USP21 with IC50 of 0.19 μM and 0.96 μM, respectively. BAY 11-7082 induces apoptosis and S phase arrest in gastric cancer cells.

Targets

E2-conjugating enzymes ^[6] (Cell-free assay)	USP7 ^[7] (Cell-free assay)	USP21 ^[7] (Cell-free assay)	USP6 ^[7] (Cell-free assay)	IκBα phosphorylation ^[1] (Tumor cells)
	0.19 μM	0.96 μM	1.7 μM	10 μM

In Vitro
In vitro	BAY 11-7082 completely and specifically abrogates NF-κB DNA binding, downregulating the NF-κB-inducible cytokine IL-6 and inducing apoptosis. ^[1] BAY 11-7082 (< 8 μM) is able to effectively inhibit both basal and TNFα stimulated NFκB luciferase activity in a dose dependent manner. BAY 11-7082 (8 μM) strongly inhibits the rate of proliferation in NCI-H1703 cells. ^[2] Bay 11-7082 (5 μM) rapidly and efficiently reduces the DNA binding of NF-kappaB in HTLV-I-infected T-cell lines and down-regulates the expression of the antiapoptotic gene, Bcl-x(L), whereas it has little effect on the DNA binding of another transcription factor, AP-1. Bay 11-7082-induced apoptosis of primary ATL cells is more prominent than that of normal peripheral blood mononuclear cells, and apoptosis of these cells is also associated with down-regulation of NF-kappaB activity. Bay 11-7082 (5 μM) selectively induces apoptosis of HTLV-I–infected T-cell lines associated with down-regulation of the expression of cyclin D1, cyclin D2, and Bcl-xL. ^[3] BAY 11-7082 (100 μM) prevents the nuclear translocation of p65 elicited by NMDA and the NMDA-induced increase of NF-κB binding in mouse hippocampal slices. BAY 11-7082 prevents NMDA toxicity occurring in CA1 region of hippocampal slices with 40% neuroprotection at 20 μM and 70% neuroprotection at 100 μM. ^[4] BAY 11-7082 at all concentrations tested significantly inhibits NF-κB p65 DNA-binding activity in adipose tissue, whereas in skeletal muscle, BAY 11-7082 at 50 μM and 100 μM significantly inhibits NF-κB p65 DNA-binding activity. BAY 11-7082 (100 μM) reduces IKK-β protein in human adipose tissue and skeletal muscle. BAY 11-7082 (100 μM) significantly decreases the release of TNF-α from adipose tissue, whereas the release of IL-6 and IL-8 is significantly inhibited at all concentrations of BAY 11-7082 tested. BAY 11-7082 (50 μM) significantly decreases the release of TNF-α, IL-6, and IL-8 in skeletal muscle. ^[5] BAY 11-7082 is also found to inactivate the E2-conjugating enzymes Ubc (ubiquitin conjugating) 13 and UbcH7 and the E3 ligase LUBAC (linear ubiquitin assembly complex), and thus induces B-cell lymphoma and leukaemic T-cell death. ^[6]
細胞実験	細胞株	NCI-H1703 cells
	濃度	~8 μM
	反応時間	12 hours
	実験の流れ	Cells are transfected with siRNA in 96-well microtiter plates and then cultured for 72 hours in complete NSCLC medium, treated with BAY 11-7082 for 12 hours. Cells are incubated with [³H]thymidine for 3 hours. The cells are collected on filters using an automatic cell harvester and radioactivity on the filters is measured by β-scintillation counting.
実験結果図	Methods	Biomarkers	結果図	PMID
	Western blot	NF-κB p-IKKβ/ IκBα p-IRAK4 / IRAK4 NF-κB p-IKKβ/ IκBα p-IRAK4 / IRAK4		31332209
	Growth inhibition assay	Cell viability Cell viability		31332209

In Vivo
In Vivo	BAY 11-7082, an NF-κB inhibitor, induces apoptosis and S phase arrest in gastric cancer cells.
動物実験	動物モデル	Male BALB/c nude mice
	投与量	2.5 & 5 mg/kg
	投与経路	i.t.

参考
[1] Melisi D, et al. Expert Opin Ther Targets, 2007, 11(2), 133-144. [2] Gastonguay A, et al. Cancer Biol Ther, 2012, 13(8), 647-656. [3] Miwatashi S, et al. J Med Chem, 2005, 48(19), 5966-5979. [4] Mori N, et al. Blood, 2002, 100(5), 1828-1834. [5] Goffi F, et al. Neurosci Lett, 2005, 377(3), 147-151. [6] Strickson S, et al. Biochem J. 2013, 451(3), 427-437. [7] Maria Stella Ritorto, et al. Nat Commun . 2014 Aug 27;5:4763. [8] Chen L, et al. J Gastroenterol. 2014 May;49(5):864-74. + 展開

参考

+ 展開

化学情報

分子量	207.25	化学式	C₁₀H₉NO₂S
CAS No.	19542-67-7	SDF	Download BAY 11-7082 SDFをダウンロードする
Smiles	CC1=CC=C(C=C1)S(=O)(=O)C=CC#N
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1个月-20°Cin solvent

In vitro
Batch:

DMSO : 41 mg/mL ( (197.82 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Ethanol : 10 mg/mL

Water : Insoluble

モル濃度計算器

in vivo Batch: Add solvents to the product individually and in order.					投与溶液組成計算機
	Clear solution	10%DMSO 1 40%PEG300 2 5%Tween80 3 45%ddH2O	4.0mg/ml	Taking the 1 mL working solution as an example, add 100 μL of 40 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 450 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.	投与溶液組成計算機
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	2.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 40 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 Corn oil	8.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 160 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):