CFSE

別名:Carboxyfluorescein succinimidyl ester, 5(6)-Carboxyfluorescein diacetate succinimidyl ester, CFDA-SE, 5(6)-CFDA N-succinmidyl ester

Carboxyfluorescein succinimidyl ester (CFSE, 5(6)-Carboxyfluorescein diacetate succinimidyl ester, CFDA-SE, 5(6)-CFDA N-succinmidyl ester) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably, to intracellular lysine residues and other amine sources.

CFSE化学構造

CAS No. 150347-59-4

サイズ 価格(税別) 在庫状況
10mM (1mL in DMSO) JPY 21800 国内在庫あり
JPY 19700 国内在庫あり
JPY 67900 国内在庫あり

代表番号: 045-509-1970|電子メール:[email protected]
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文献中Selleckの製品使用例(15)

製品安全説明書

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99.85

CFSE関連製品

Dyes阻害剤の選択性比較

生物活性

製品説明 Carboxyfluorescein succinimidyl ester (CFSE, 5(6)-Carboxyfluorescein diacetate succinimidyl ester, CFDA-SE, 5(6)-CFDA N-succinmidyl ester) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably, to intracellular lysine residues and other amine sources.
In Vitro
In vitro

Preparation of CFDA-SE working solution
1.1 Preparation of the stock solution
Dissolve 1 mg of CFDA-SE in 0.1794 mL of DMSO to obtain 10 mM of CFSE.
Note: It is recommended to store the stock solution at -20 ℃ or -80 ℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of CFDA-SE working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of CFDA-SE working solution.
Note: Please adjust the concentration of CFDA-SE working solution according to the actual situation.
Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of CFDA-SE working solution, and then incubate at room temperature for 30 minutes.
2.3 Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

細胞実験 細胞株 human erythroleukaemic cell line K562, mouse lymphoma cell line YAC-1, human mammary cancer cell line MCF-7 and human melanoma cell line A375
濃度 2 µM, 3 µM, 4 µM, 5 µM, 10 µM and 20 µM
反応時間 5, 6, 7, 8, 10 and 15 min
実験の流れ

The 5 mM CFDA-SE stock in DMSO is diluted to different concentrations(2 µM, 3 µM, 4 µM, 5 µM, 10 µM and 20 µM) in PBS with a total volume of 1 ml. After each cell line is harvested and washed three times with PBS, 1×109 cells are added to equal volume of CFDA-SE with different concentrations and incubated at 37°C for 5, 6, 7, 8, 10 and 15 min with agitation. The labeling reaction is stopped for 1 min by adding an equal volume of heat inactivated fetal bovine serum. The CFDA-SE labeled cells are washed twice with PBS and recounted, and the cell concentration is adjusted to 6×104cells/ml in IMDM containing 10% FCS.

In Vivo
In Vivo

In vivo labeling of cells with CFSE is feasible with virtually no toxic effect on the labeled or adjacent cells. It has advantages in relatively long-term migration studies as demonstrated by the recovery of T-cells in the peripheral lymphoid organs[2].

動物実験 動物モデル C57Bl/6 mice
投与量 10 μM
投与経路 injected into thymic lobe
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02028403 Completed
HIV Infections
National Institute of Allergy and Infectious Diseases (NIAID)
June 2014 Phase 1
NCT01376726 Completed
HIV Infections
National Institute of Allergy and Infectious Diseases (NIAID)|Novartis Vaccines and Diagnostics Inc
July 2011 Phase 1
NCT01029548 Completed
HIV Infections
Barbara Ensoli MD|Istituto Superiore di Sanità
April 2008 --
NCT01024556 Completed
HIV Infection
Barbara Ensoli MD|Istituto Superiore di Sanità
March 2008 --

化学情報

分子量 557.46 化学式

C29H19NO11

CAS No. 150347-59-4 SDF Download CFSE SDFをダウンロードする
Smiles CC(=O)OC1=CC=C2C(=C1)OC3=C(C=CC(=C3)OC(C)=O)C24OC(=O)C5=CC(=CC=C45)C(=O)ON6C(=O)CCC6=O.CC(=O)OC7=CC=C8C(=C7)OC9=C(C=CC(=C9)OC(C)=O)C8%10OC(=O)C%11=CC=C(C=C%10%11)C(=O)ON%12C(=O)CCC%12=O
保管 3 years -20°C(in the dark) powder

In vitro
Batch:

DMSO : 100 mg/mL ( (179.38 mM); 吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : Insoluble

Ethanol : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量 濃度 体積 分子量

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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Handling Instructions

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