Digoxin

Digoxin is a classical Na,K-ATPase inhibitor, with selectivity for the α2β3 isoform over the common α1β1 isoform, used in the treatment of atrial fibrillation and heart failure.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.

CAS No. 20830-75-5

サイズ	価格(税別)	在庫状況
100mg	JPY 22000	国内在庫あり
500mg	JPY 44500	国内在庫あり

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

文献中Selleckの製品使用例（12）

製品安全説明書

現在のバッチを見る：純度： 99.61%

99.61

Digoxin関連製品

関連ターゲット	V-ATPase F-ATPase SERCA RecA AAA ATPase family	もっと見る
関連製品	Brefeldin A (BFA) (-)-Blebbistatin CB-5083 Omecamtiv mecarbil (CK-1827452) Thapsigargin Sodium orthovanadate Golgicide A Esomeprazole sodium Ciclopirox ethanolamine Oleic Acid Bufalin Ciclopirox Bedaquiline BTB06584 Ginsenoside Rb1 CDN1163 Pantoprazole sodium hydrate Periplocin Chlorpropamide Rhodamine 123 tetrathiomolybdate Saikosaponin D	もっと見る
関連化合物ライブラリー	FDA-approved Drug Library Natural Product Library Ion Channel Ligand Library Exosome Secretion Related Compound Library Calcium Channel Blocker Library	もっと見る

シグナル伝達経路

ATPaseシグナル伝達経路

ATPase阻害剤の選択性比較

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	活性情報	PMID
Hs578Bst	Cytotoxicity assay	40 uM	72 hrs	Cytotoxicity against human Hs578Bst cells at 40 uM after 72 hrs by CellTiter-Glo assay relative to untreated control, IC50=47.7μM	22316168
HeLa	Function assay	150 nM	24 hrs	Inhibition of Na(+)/K(+) ATPase in human HeLa cells at 150 nM after 24 hrs by immunoblotting	26122772
HeLa	Function assay	150 nM	24 hrs	Inhibition of Na(+)/K(+) ATPase in human HeLa cells at 150 nM after 24 hrs by immunoblotting	26122772
HeLa	Function assay	150 nM	24 hrs	Inhibition of Na(+)/K(+) ATPase in human HeLa cells at 150 nM after 24 hrs by immunoblotting	26122772
HeLa	Function assay	150 nM	24 hrs	Inhibition of Na(+)/K(+) ATPase in human HeLa cells at 150 nM after 24 hrs by immunoblotting	26122772
HeLa	Function assay	150 nM	24 hrs	Inhibition of Na(+)/K(+) ATPase in human HeLa cells at 150 nM after 24 hrs by immunoblotting	26122772
HeLa	Function assay	150 nM	24 hrs	Inhibition of Na(+)/K(+) ATPase in human HeLa cells at 150 nM after 24 hrs by immunoblotting	26122772
HeLa	Function assay	150 nM	24 hrs	Inhibition of Na(+)/K(+) ATPase in human HeLa cells at 150 nM after 24 hrs by immunoblotting	26122772
HeLa	Function assay	150 nM	24 hrs	Inhibition of Na(+)/K(+) ATPase in human HeLa cells at 150 nM after 24 hrs by immunoblotting	26122772
DU145	Function assay	50 uM	5 hrs	Inhibition of Na+/K+ ATPase pump in human DU145 cells assessed as increase in intracellular calcium level at 50 uM measured after 5 hrs by Fura-2/AM dye based fluorescence assay	31120251
DU145	Function assay	50 uM	5 hrs	Inhibition of Na+/K+ ATPase pump in human DU145 cells assessed as increase in intracellular calcium level at 50 uM measured after 5 hrs by Fura-2/AM dye based fluorescence assay	31120251
DU145	Function assay	50 uM	5 hrs	Inhibition of Na+/K+ ATPase pump in human DU145 cells assessed as increase in intracellular calcium level at 50 uM measured after 5 hrs by Fura-2/AM dye based fluorescence assay	31120251
DU145	Function assay	50 uM	5 hrs	Inhibition of Na+/K+ ATPase pump in human DU145 cells assessed as increase in intracellular calcium level at 50 uM measured after 5 hrs by Fura-2/AM dye based fluorescence assay	31120251
DU145	Function assay	50 uM	5 hrs	Inhibition of Na+/K+ ATPase pump in human DU145 cells assessed as increase in intracellular calcium level at 50 uM measured after 5 hrs by Fura-2/AM dye based fluorescence assay	31120251
DU145	Function assay	50 uM	5 hrs	Inhibition of Na+/K+ ATPase pump in human DU145 cells assessed as increase in intracellular calcium level at 50 uM measured after 5 hrs by Fura-2/AM dye based fluorescence assay	31120251
DU145	Function assay	50 uM	5 hrs	Inhibition of Na+/K+ ATPase pump in human DU145 cells assessed as increase in intracellular calcium level at 50 uM measured after 5 hrs by Fura-2/AM dye based fluorescence assay	31120251
DU145	Function assay	50 uM	5 hrs	Inhibition of Na+/K+ ATPase pump in human DU145 cells assessed as increase in intracellular calcium level at 50 uM measured after 5 hrs by Fura-2/AM dye based fluorescence assay	31120251
human hepatocytes	Function assay	0.05 to 10 uM		Drug uptake in sandwich-cultured human hepatocytes at 0.05 to 10 uM at 37 degC by Michaelis-Menten kinetics/scintillation spectroscopy, Km=2.39 μM	20926619
HCT116	Cytotoxicity assay		72 hrs	Cytotoxicity against human HCT116 cells after 72 hrs by FMCA method, IC50=0.27μM	19894733
CC20	Cytotoxicity assay		72 hrs	Cytotoxicity against human CC20 cells after 72 hrs by FMCA method, IC50=0.24μM	19894733
colon cancer	Cytotoxicity assay		72 hrs	Cytotoxicity against human colon cancer cells after 72 hrs by FMCA method, IC50=0.1μM	19894733
UACC62	Cytotoxicity assay		48 hrs	Cytotoxicity against human UACC62 cells after 48 hrs by SRB assay, IC50=0.0295μM	16309315
MCF7	Cytotoxicity assay		48 hrs	Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay, IC50=0.0241μM	16309315
TK10	Cytotoxicity assay		48 hrs	Cytotoxicity against human TK10 cells after 48 hrs by SRB assay, IC50=0.0146μM	16309315
HT-29	Cytotoxicity assay		72 hrs	Cytotoxicity against human HT-29 cells after 72 hrs by FMCA method, IC50=1.4μM	19894733
Hs578Bst	Cytotoxicity assay		72 hrs	Cytotoxicity against human Hs578Bst cells after 72 hrs by CellTiter-Glo assay, IC50=0.04μM	22316168
Hs578T	Cytotoxicity assay		72 hrs	Cytotoxicity against human Hs578T cells after 72 hrs by CellTiter-Glo assay, IC50=0.251μM	22316168
T47D	Cytotoxicity assay		72 hrs	Cytotoxicity against human T47D cells after 72 hrs by CellTiter-Glo assay, IC50=0.66μM	22316168
SK-BR-3	Cytotoxicity assay		72 hrs	Cytotoxicity against human SK-BR-3 cells after 72 hrs by CellTiter-Glo assay, IC50=1.08μM	22316168
MCF7	Cytotoxicity assay		72 hrs	Cytotoxicity against human MCF7 cells after 72 hrs by CellTiter-Glo assay, IC50=4.7μM	22316168
Hs683	Cytotoxicity assay		3 days	Cytotoxicity against human Hs683 cells after 3 days by MTT assay, IC50=0.04μM	23706005
A549	Cytotoxicity assay		3 days	Cytotoxicity against human A549 cells after 3 days by MTT assay, IC50=0.05μM	23706005
PC3	Cytotoxicity assay		3 days	Cytotoxicity against human PC3 cells after 3 days by MTT assay, IC50=0.075μM	23706005
U373	Cytotoxicity assay		3 days	Cytotoxicity against human U373 cells after 3 days by MTT assay, IC50=0.146μM	23706005
SK-MEL-28	Cytotoxicity assay		3 days	Cytotoxicity against human SK-MEL-28 cells after 3 days by MTT assay, IC50=0.266μM	23706005
MCF7	Cytotoxicity assay		3 days	Cytotoxicity against human MCF7 cells after 3 days by MTT assay, IC50=0.426μM	23706005
RKO-AS45-1	Cytotoxicity assay		48 hrs	Cytotoxicity against human RKO-AS45-1 cells after 48 hrs by MTT assay, IC50=0.42μM	26122772
HeLa	Cytotoxicity assay		48 hrs	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay, IC50=2.2μM	26122772
Kasumi-1	Cytotoxicity assay		48 hrs	Cytotoxicity against human Kasumi-1 cells incubated for 48 hrs by MTS assay, IC50=0.093μM	27983842
MV4-11	Cytotoxicity assay		48 hrs	Cytotoxicity against human MV4-11 cells incubated for 48 hrs by MTS assay, IC50=0.111μM	27983842
HT-29	Cytotoxicity assay		72 hrs	Cytotoxicity against human HT-29 cells incubated for 72 hrs by SRB assay, IC50=0.38μM	27983842
H1299	Cytotoxicity assay		24 hrs	Cytotoxicity against human H1299 cells incubated for 24 hrs by MTT assay, IC50=0.46μM	27983842
CCD-112CoN	Cytotoxicity assay		72 hrs	Cytotoxicity against human CCD-112CoN cells incubated for 72 hrs, IC50=2.4μM	27983842
HeLa	Function assay			Inhibition of mitosis in human HeLa cells by imaging analysis	18066055
HeLa	Cytotoxicity assay			Cytotoxicity against human HeLa cells assessed as inhibition of DNA replication by imaging analysis	18066055
K562	Cytotoxicity assay			Cytotoxicity against human K562 cells by XTT assay, IC50=0.0282μM	16309315
MDCK	Function assay			TP_TRANSPORTER: cell accumulation in OATP4C1-expressing MDCK cells, Km=8μM	14993604
MDCK	Function assay			TP_TRANSPORTER: cell accumulation in OATP4C1-expressing MDCK cells, Km=7.8μM	14993604
LLC-PK1	Function assay			TP_TRANSPORTER: uptake in Oatp2-expressing LLC-PK1 cells, Km=0.279μM	11883641
LLC-PK1	Function assay			TP_TRANSPORTER: uptake in Oatp2-expressing LLC-PK1 cells, Km=1.07μM	11792694
LLC-PK1	Function assay			TP_TRANSPORTER: inhibition of Taurocholate uptake in Oatp2-expressing LLC-PK1 cells, Ki=0.58μM	11792694
High Five (BTI-TN5B1-4)	Function assay			TP_TRANSPORTER: ATP hydrolysis in membrane fraction from High Five (BTI-TN5B1-4) cells, Km=25.9μM	11785684
LLC-PK1	Function assay			TP_TRANSPORTER: inhibition of E217betaG uptake in Oatp2-expressing LLC-PK1 cells, Ki=0.037μM	11408557
rat hepatocytes	Function assay			Drug uptake in rat hepatocytes, Km=0.18 μM	20926619
CHO	Function assay			pIC50 values for sodium fluorescein (10 uM) uptake in OATP1B1-transfected CHO cells, IC50=3.23594μM	23571415
CHO	Function assay			pIC50 values for sodium fluorescein (10 uM) uptake in OATP1B3-transfected CHO cells, IC50=13.1826μM	23571415
Drosophila Schneider cells	Function assay			Antagonist activity at transactivation domain of RORgammat (unknown origin) expressed in Drosophila Schneider cells co-expressing Gal4-DNA binding domain, IC50=2μM	24040486
Drosophila Schneider cells	Function assay			Antagonist activity at transactivation domain of RORgammat (unknown origin) expressed in Drosophila Schneider cells co-expressing Gal4-DNA binding domain, IC50=2μM	24040486
他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

生物活性

製品説明

Digoxin is a classical Na,K-ATPase inhibitor, with selectivity for the α2β3 isoform over the common α1β1 isoform, used in the treatment of atrial fibrillation and heart failure.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.

Targets

Na,K-ATPase α2β3 ^[1]	Na,K-ATPase α2β1 ^[1]	Na,K-ATPase α1β1 ^[1]
42.8 nM(Ki)	58.7 nM(Ki)	268 nM(Ki)

参考
[1] Katz A, et al. Proc Natl Acad Sci U S A. 2015, 112(44):13723-8.

参考

[1] Katz A, et al. Proc Natl Acad Sci U S A. 2015, 112(44):13723-8.

化学情報

分子量	780.94	化学式	C₄₁H₆₄O₁₄
CAS No.	20830-75-5	SDF	Download Digoxin SDFをダウンロードする
Smiles	CC1C(C(CC(O1)OC2C(OC(CC2O)OC3C(OC(CC3O)OC4CCC5(C(C4)CCC6C5CC(C7(C6(CCC7C8=CC(=O)OC8)O)C)O)C)C)C)O)O
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1个月-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL ( (128.05 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : Insoluble

Ethanol : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):