Gefitinib (ZD1839)

製品コードS1025

Gefitinib (ZD1839)化学構造

分子量(MW):446.90

Gefitinib (ZD-1839)は一種のEGFR阻害剤で、NR6wtEGFRとNR6Wの細胞中のTyr1173、Tyr992、Tyr1173とTyr992に作用する時のIC50値が37 nM、37nM、26 nMと57 nMにそれぞれ分かれることです。

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文献中の引用(91)

カスタマーフィードバック(12)

  • Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

    Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

  • (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

    LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

  • Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

製品安全説明書

EGFR阻害剤の選択性比較

生物活性

製品説明 Gefitinib (ZD-1839)は一種のEGFR阻害剤で、NR6wtEGFRとNR6Wの細胞中のTyr1173、Tyr992、Tyr1173とTyr992に作用する時のIC50値が37 nM、37nM、26 nMと57 nMにそれぞれ分かれることです。
特性 A potent EGFR tyrosine kinase inhibitor.
ターゲット
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
体外試験

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB NV\wRZR2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIfFdW1KSzVyPUCuNFU1KM7:TR?= M3HjRVE3PTF4NEez
NIH3T3 NEHIPWRHfW6ldHnvckBCe3OjeR?= MWOwMlIh|ryP MVeyJIg> MYfEUXNQ NG\XTmZKdmirYnn0bY9vKG:oIFXHSkB{fGmvdXzheIVlKGi3bXHuJIVz[kJzIHH1eI9xcG:|cHjvdplt[XSrb36ge4l1cCCLQ{WwJI9nKDBwMEG0OEDPxE1? NVzMWpY3OTZ2OECyPFQ>
COS7 MnTqSpVv[3Srb36gRZN{[Xl? NFTYNWg6PiEQvF2= M4jlemlvcGmkaYTpc44hd2ZiaIXtZY4hUkGNMjDWOlE4TiCvdYThcpQh\XiycnXzd4Vl MVyxO|E4QDd{Mh?=
A549 MUDDfZRwfG:6aXOgRZN{[Xl? M37PRVk3KGh? MmTTSG1UVw>? M2fMRWlEPTB;MUOuOVkh|ryP MoTKNVg{OTN6MEe=
K562 NHu1foVEgXSxdH;4bYMhSXO|YYm= NXHUcVhWQTZiaB?= MoLkSG1UVw>? Mm\STWM2OD17LkO2JO69VQ>? MoHTNVg{OTN6MEe=
MDA-MB-231 M3XzTGN6fG:2b4jpZ{BCe3OjeR?= M4DzbFk3KGh? MVnEUXNQ MUDJR|UxRTN4Lk[xJO69VQ>? MXyxPFMyOzhyNx?=
MOLT4 MVvDfZRwfG:6aXOgRZN{[Xl? NXPKcJlUQTZiaB?= MlfJSG1UVw>? Mn\JTWM2OD1zNT6wNkDPxE1? NXvWTnVZOTh|MUO4NFc>
MCF7 MYPDfZRwfG:6aXOgRZN{[Xl? NECx[Ik2OCEQvF2= NGLKS|M4OiCq NF3hbFVKSzVyPUGyMlA2KM7:TR?= Mmf4NVg4PzF6MUm=
BT474 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIHLVVY4OiCq MnrPRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCEVES3OEBk\WyuczDveoVz\XiycnXzd4lv\yCHUlLiNkB4cXSqIFXDOVAhd2ZiMD6zJO69VQ>? MnixNVkxOjh2MkS=
HN5 M1XL[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnz0O|IhcA>? NXPBSYd5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUlUh[2WubIOgc5ZmemW6cILld5NqdmdiRVfGVkB4cXSqIFXDOVAhd2ZiMD6wNUDPxE1? MknaNVkxOjh2MkS=
BA/F3 Ml\ER5l1d3SxeHnjJGF{e2G7 NEC0[5k4OiCq M{PvU2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGJCN0Z|IHPlcIx{KGW6cILld5NqdmdiRVfGVkBNQDV6UjDteZRidnRid3n0bEBKSzVyIH;mJFAvODFyODFOwG0> M1r4SFE6OjN7MkK5
NIH3T3 M3f4[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHP4Z5c4OiCq Mm\jS5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgUmlJNzOWMzDj[YxteyCneIDy[ZN{cW6pIHXyRlIh\2WwZTD3bZRpKEmFNUCgc4YhOC53NDFOwG0> NILMcVEyQTZ4NUO3Oy=>
MCF7 MX MVTGeY5kfGmxbjDBd5NigQ>? Mlm0N|AhdWmw NYLVVXZTUW6qaXLpeIlwdiCxZjDCR3JRKGW6cILld5Nm\CC5aYToJGlEPTBib3[gNU4yKM7:TR?= MmXjNVk6OzJ7NkC=
A431 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1zs[|ExKM7:TR?= NWfydVR[PzJiaB?= NUfQb5ExT0l3ME2wMlUyPCEQvF2= NIPKcpcyQTl4OUS2OS=>
A431 Mn3sT4lv[XOnIFHzd4F6 NHX0OVIxNjFizszN NUHGV|NnOSCq NHTrbZNKdmirYnn0bY9vKG:oIFXHSk1qdmS3Y3XkJGVITlJicHjvd5Bpd3K7bHH0bY9v NXHRUmo2OTl7Nkm0OlU>
A431 M2LSVWtqdmG|ZTDBd5NigQ>? MXexNFAh|ryP NVnnZWNFOTBibXnu MYTQdo9td26pZXSgbY5pcWKrdH;yfUBi[3Srdnn0fUBw\iCHR1[tbY5lfWOnZDDFS2ZTKHCqb4PwbI9zgWyjdHnvci=> M3P1SVE6QTZ7NE[1
Sf9 M{jJZWtqdmG|ZTDBd5NigQ>? NITyXYcyOCCvaX6= MnfCSG1UVw>? NXHUVWNRUW6qaXLpeIlwdiCxZjDFS2ZTKGW6cILld5Nm\CCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHKnY3XweI9zKGG3dH;wbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFAvODN|IN88US=> MnuxNlAxPTZ2MkW=
NCI-H1975 Mnj1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEX0NYQ2KM7:TR?= MlnhO|IhcA>? NIjMfHBKSzVyPUiuNlYh|ryP NHvvR4UzODF3MU[3NC=>
NCI-H1975 NVHUbXJ5U2mwYYPlJGF{e2G7 M3HSdlExKM7:TR?= MlnITY5pcWKrdHnvckBw\iCHR1\SJIF2fG:yaH;zdIhwenmuYYTpc44> MYqyNFE2OTZ5MB?=
NCI-H1975 MWnGeY5kfGmxbjDBd5NigQ>? M17uTlExKM7:TR?= M{TzR2lvcGmkaYTpc44hd2ZiRWLCRlIh[XW2b4Doc5NxcG:{eXzheIlwdg>? Mkj5NlAyPTF4N{C=
NCI-H1975 M1jlV2Z2dmO2aX;uJGF{e2G7 M{fFZlUh|ryP MYq0PEBp M4HJXWlv\HWldHnvckBw\iCjcH;weI9{cXNiYYPz[ZN{\WRiYYOgZYN1cX[jdHnvckBw\iClYYPwZZNmNTN? MWiyNFE2OTZ5MB?=
A375 Mn\sS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1nYXlQ5KGh? MYDJR|UxRTJyLkW1JO69VQ>? M4fMO|IxOzB2NUO3
SMMC7721 M17zZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2HKUVQ5KGh? NUPt[YZGUUN3ME2zN{4yOiEQvF2= M2r1cFIxOzB2NUO3
DU145 NFXtRWlEgXSxdH;4bYMhSXO|YYm= NVPCZox2PSCm NHm0bYNFVVOR NYGwbJlJUUN3ME20Nk4yPyEQvF2= MYWyNFQ3PjV3NR?=
MIAPaCa2 MVXDfZRwfG:6aXOgRZN{[Xl? Mn7MOUBl NF7G[4RFVVOR NUmzNGFbUUN3ME21NE43OiEQvF2= M1\OTFIxPDZ4NUW1
PANC1 MkjlR5l1d3SxeHnjJGF{e2G7 M1;zXFUh\A>? NGPNZpJFVVOR NFrVUmFKSzVyPUSwMlQzKM7:TR?= NGnQeFQzODR4NkW1OS=>
PC9 NFW1botIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFXHSnIu\GWoaXPp[Y51KGi3bXHuJHBEQSClZXzsd{B4cXSqIFXDOVAhd2ZiMD6wNVch|ryP MVKyNVIxQDhyMh?=
PC9 Mmm1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnnbpl2SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBoem:5dHitdoV{cXO2YX70JIh2dWGwIGDDPUBk\WyuczDlfJBz\XO|aX7nJGVITlJiRUe0On9CPzVyL2S3PVBOKG23dHHueEB4cXSqIFXDOVAhd2ZiNzFOwG0> MYOyNVIxQDhyMh?=
PC3 MnywS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUOxNEDPxE1? MljnO|IhcA>? MWLEUXNQ NEX5dodKSzVyPUeuOEDPxE1? M1fKV|IyOzV|NUS2
MDA-MB-468 NULt[YRFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXzqNXNqOTByIN88US=> MWm0PEBp MW\EUXNQ NXvhWZh[SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJk> NXjqXHA3OjF2Mkm2N|I>
Plasmodium falciparum M4i1XZFJXFNicILv[oltcW6pIH\vdkBqdmirYnn0c5J{KG:oIGDsZZNud2SrdX2g[oFt[2myYYL1cUBxem:uaX\ldoF1cW:w NGrFO|UzOThzN{C0OS=>
NCI-H1993 M1rZOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M37MblczKGh? NHjUPZVFVVOR NWrUU29sUUN3ME2wMlEh|ryP NHLuOXozOTlzNkSzNy=>
NCI-H292 M2fLZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIrnb2w4OiCq MnTTSG1UVw>? NFLhW4lKSzVyPUCuNFIh|ryP M4jueVIyQTF4NEOz
A431 M3Gxe2tqdmG|ZTDBd5NigQ>? M1nOPFIhcA>? MonDSG1UVw>? MVLJcohq[mm2aX;uJI9nKEWJRmKgdIhwe3Cqb4L5cIF1cW:wIHnuJGVITi2|dHnteYxifGWmIHj1cYFvKEF2M{Gge4l1cCCLQ{WwJI9nKDBwMECxJO69VQ>? MXuyNlExOTF|Mh?=
MCF7 NUXadZFKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkflNVAxyqEQvHevcWw> NHrVfnY1QCCq NYe0V5V3TE2VTx?= Mkm5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWJRmKg[4Vv\SC5aYToJGlEPTBib3[gNVIvODVizszN M1;JSVIzOTF7MUOw
BT474 MYTDfZRwfG:6aXOgRZN{[Xl? NGO3NlEyOCEQvF2= NILBOXg1QCCq MoDNR5l1d3SxeHnjbZR6KGGpYXnud5Qhe2W{dX2td5Rien[nZDDoeY1idiCEVES3OEBk\Wyucx?= NULwTpNwOjJzM{izNFg>
MCF7 NEDMZ5BEgXSxdH;4bYMhSXO|YYm= MXexNEDPxE1? NELXRWw1QCCq NIDJ[G1EgXSxdH;4bYNqfHliYXfhbY5{fCC|ZYL1cU1{fGG{dnXkJIh2dWGwIF3DSlch[2WubIOgc5Zmei2neIDy[ZN{cW6pIFXyZmIzKGOnbHzz NHqyeI4zOjF|OEOwPC=>
HCC827 MYXLbY5ie2ViQYPzZZk> NUjjW2pzOC5zMkWg{txO M2ewdVI1KGh? M3fEUGROW09? MYHJcohq[mm2aX;uJI9nKEWJRmKg[IVtKEV5NE[tRVc2OCCvdYThcpQhYTFyNkigZZV1d3Cqb4PwbI9zgWyjdHnvci=> MmDaNlI{Ozl|NEK=
HCC827 Mmq2T4lv[XOnIFHzd4F6 NEHnWYsxNjF{NTFOwG0> MXWyOEBp NGnYVHNFVVOR MlrOTY5pcWKrdHnvckBw\iCHR1\SJIRmdCCHN{S2MWE4PTBibYX0ZY51NW2nZHnheIVlKEW{azDwbI9{eGixconsZZRqd25? M2TzNFIzOzN7M{Sy
HCC827 Mo[1T4lv[XOnIFHzd4F6 MX6wMlEzPSEQvF2= NYDSUWMyOjRiaB?= MmiySG1UVw>? NGnQXWNKdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRvbXXkbYF1\WRiQXv0JJBpd3OyaH;yfYxifGmxbh?= MmTJNlI{Ozl|NEK=
A431 NIjNZW5McW6jc3WgRZN{[Xl? MVqxNFAh|ryP NU\YcWN6OzBibXnu MnjTTY5pcWKrdHnvckBw\iCHR1\SJIRmemm4ZXSg[pJwdSCqdX3hckBCPDNzIHPlcIwhdWWvYoLhcoUh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBxd2y7LVfseU1VgXJicHjvd5Bpd3K7bHH0bY9vKHerdHigTWM2OCCxZjCxMlA5KM7:TR?= MVuyNlY6QDd6Mh?=
H460 MYnDfZRwfG:6aXOgRZN{[Xl? NXPnTGJYPzJiaB?= MWjJR|UxRTVwNUmg{txO NWPkeJhoOjJ5Mke0OFk>
HT-29 NWnkSIxiS3m2b4TvfIlkKEG|c3H5 NX;tTY05PzJiaB?= NYfLUpdyUUN3ME2zMlM3KM7:TR?= Ml3YNlI4Ojd2NEm=
SGC7901 MVnDfZRwfG:6aXOgRZN{[Xl? M4PlS|czKGh? M4XYc2lEPTB;MUCuNlYh|ryP NELvTG8zOjd{N{S0PS=>
A498 NHLJfmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjwT5p4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOFk5KGOnbHzzJIV5eHKnc4PpcochTUeIUjDhcoQhW1KFIIfpeIghUUN3MDDv[kA5NjZizszN NETpfIMzOjhzOEi0PC=>
Calu3 NXvGW4JRS3m2b4TvfIlkKEG|c3H5 MXGxNEDPxGdxbVy= NF3EUI04OiCq NWLTZ4tpUUN3ME2xMlYh|ryP MVuyN|EyPjF4OB?=
FADU NXTXfoI3S3m2b4TvfIlkKEG|c3H5 MWWxNEDPxGdxbVy= NXjyc|FpPzJiaB?= NXPScVhNUUN3ME2wMlA2KM7:TR?= MnnLNlMyOTZzNki=
HCC827 NHfacmxEgXSxdH;4bYMhSXO|YYm= MmDqNVAh|rypL33M MmHwO|IhcA>? NXTudYVtUUN3ME2wMlAxOTVizszN MkXyNlMyOTZzNki=
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JVM-3 NIDzeWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NITyRoRKSzVyPUKuN|Q3PDNizszN MVTTRW5ITVJ?
NCI-H2170 NFvUV5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfETWM2OD1{LkO0O|g{KM7:TR?= M2DpT3NCVkeHUh?=
EW-18 NIG2T5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVn2dWJ2UUN3ME2yMlM2PDdzIN88US=> NYnUcIdjW0GQR1XS
KU812 NVXaPY5XT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3nyRmlEPTB;Mj6zOlc4QSEQvF2= MXHTRW5ITVJ?
NY MlHoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV\JR|UxRTJwM{eyO{DPxE1? MUPTRW5ITVJ?
HTC-C3 M122XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4jQ[2lEPTB;Mj60NlI3PyEQvF2= M1\SNHNCVkeHUh?=
CFPAC-1 NFrrS5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{i1SmlEPTB;Mj60N|g3PCEQvF2= NYfLPGl5W0GQR1XS
HH NHnjfGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUPuVJR1UUN3ME2yMlQ6QDV2IN88US=> NGj6dZlUSU6JRWK=
MKN7 MmfNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfyfmI1UUN3ME2yMlYzPzR6IN88US=> NHS3[I9USU6JRWK=
TE-8 NHXWVoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH\SVppKSzVyPUKuOlM3PTNizszN NYWxNYdZW0GQR1XS
MC-IXC MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWTVXmd[UUN3ME2yMlY1PDV|IN88US=> NUW4cJdRW0GQR1XS
DMS-79 NFv0U3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGHoSWhKSzVyPUKuOlkzQDRizszN MlW4V2FPT0WU
Detroit562 NEXQ[nZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGPZZpBKSzVyPUKuOlk5PDVizszN M3XkdnNCVkeHUh?=
HuP-T4 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXzJR|UxRTJwN{K4NVgh|ryP MkH0V2FPT0WU
QIMR-WIL NFWyeIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;XWGtMUUN3ME2yMlc2QTV{IN88US=> MkjsV2FPT0WU
BB65-RCC M2HJbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1XaeWlEPTB;Mj64NFcyOyEQvF2= NFnXN4ZUSU6JRWK=
PC-3 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;xTWM2OD1{LkizN|E4KM7:TR?= MYTTRW5ITVJ?
OVCAR-3 NW\FN415T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{XvWGlEPTB;Mj64OVU6PCEQvF2= NULL[5VCW0GQR1XS
KYSE-510 M37FNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFHXZ5RKSzVyPUKuPFY4QDZizszN M{nOU3NCVkeHUh?=
HC-1 NIrZcldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1Tp[2lEPTB;Mj65OFAyOiEQvF2= MnXNV2FPT0WU
NCI-H1693 M4DacGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn;STWM2OD1{Lkm1PFk4KM7:TR?= NXX3VGd{W0GQR1XS
DU-4475 MlPsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTJwOUmwOVIh|ryP M3j5fHNCVkeHUh?=
COLO-680N NVHSNpF7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXTJR|UxRTNwMEC0PVUh|ryP MYXTRW5ITVJ?
MN-60 NFLkbnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE\1RYFKSzVyPUOuNFQ6PzRizszN MXTTRW5ITVJ?
BCPAP NH6xfVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTNwMEiyPVUh|ryP M4nCVXNCVkeHUh?=
647-V NFL3co9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUHWTIZvUUN3ME2zMlE3OjJizszN NEXzOo1USU6JRWK=
HT-1376 M2G2fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX7JR|UxRTNwMkC1NFYh|ryP NIrVcmVUSU6JRWK=
NCI-H1793 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3LXZ2lEPTB;Mz6yNVA6QCEQvF2= NFGzWHZUSU6JRWK=
SW1463 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nhNGlEPTB;Mz6yOlE6OiEQvF2= NHPObYlUSU6JRWK=
NCI-H727 M3XFSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjCdo1QUUN3ME2zMlM5PTh2IN88US=> MUjTRW5ITVJ?
LB771-HNC NFO3UnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjnR2ZKSzVyPUOuN|kyOjZizszN NYf1cJV{W0GQR1XS
D-542MG MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXCTWM2OD1|LkS0NlIzKM7:TR?= NVyyPZdDW0GQR1XS
NTERA-S-cl-D1 NV\JVpFQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPVTWM2OD1|LkS0PVU4KM7:TR?= NVK5fHFMW0GQR1XS
C8166 NUSxWZE{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUfwU3hvUUN3ME2zMlQ3PTJzIN88US=> MoDwV2FPT0WU
639-V NFPmUmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYnYcHFWUUN3ME2zMlU4Pzl{IN88US=> NVr4dZo{W0GQR1XS
TE-1 NEi2TmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHnPfmtKSzVyPUOuOVkyPjlizszN MV;TRW5ITVJ?
OAW-28 NFzRWohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHVSJV5UUN3ME2zMlU6PDJ4IN88US=> NGXi[5RUSU6JRWK=
SK-LU-1 NIG1cZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTNwNkC2OFUh|ryP MX3TRW5ITVJ?
CaR-1 M{fabWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVHJR|UxRTNwNkGwOFgh|ryP M{nrcnNCVkeHUh?=
A2780 M4\wXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\JTWM2OD1|Lk[0NFg6KM7:TR?= NWH2S4Y4W0GQR1XS
C2BBe1 MmrlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzFTWM2OD1|Lk[0OFA4KM7:TR?= NUG3RoRuW0GQR1XS
SK-MES-1 M4nSOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTXTphJUUN3ME2zMlY1PjF2IN88US=> NEGyO45USU6JRWK=
PANC-10-05 NVS4Tm0{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVPCRol[UUN3ME2zMlczODd{IN88US=> MoHJV2FPT0WU
J-RT3-T3-5 M2rzTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLpTWM2OD1|Lke1OFA3KM7:TR?= M2LxTnNCVkeHUh?=
ES4 NH\XUm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33rUmlEPTB;Mz64NlI5PiEQvF2= M33ze3NCVkeHUh?=
NCI-H28 NXL5fpJPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIjteWNKSzVyPUOuPFI6PjFizszN MlLYV2FPT0WU
NCI-H1155 NUewe5NYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvze49[UUN3ME2zMlg1OTV7IN88US=> NGT3NFdUSU6JRWK=
KURAMOCHI MlXwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYDBXIx[UUN3ME2zMlg3ODV4IN88US=> MnGwV2FPT0WU
NCI-H69 M37pcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUTFVHhkUUN3ME2zMlkxOTd6IN88US=> MUTTRW5ITVJ?
IST-MES1 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1jKVWlEPTB;Mz65OFAzQSEQvF2= M{fMPXNCVkeHUh?=
NH-12 M3HPZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHTpRYFKSzVyPUOuPVQyQDdizszN M2rpcXNCVkeHUh?=
JVM-2 M3;u[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnjrTWM2OD1|Lkm0O|UzKM7:TR?= MX\TRW5ITVJ?
EW-16 M4fsTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfDOWR2UUN3ME2zMlk2PTd5IN88US=> MXLTRW5ITVJ?
NCI-H441 NEjufYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTmOnNEUUN3ME2zMlk4ODF2IN88US=> NVfGeYJKW0GQR1XS
RCC10RGB NH;nN3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIixfoZKSzVyPUSuNFEzPDZizszN NGLoe4JUSU6JRWK=
TCCSUP M4Hhemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvIZmtKSzVyPUSuNVExPSEQvF2= MmPiV2FPT0WU
HL-60 Mlj2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWnYUoRsUUN3ME20MlEzOjRzIN88US=> Mnr6V2FPT0WU
EW-11 Mn;iS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4DDcWlEPTB;ND6xN|I3OSEQvF2= MWDTRW5ITVJ?
KARPAS-299 NYTYT2QzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmHUTWM2OD12LkOxNFE{KM7:TR?= Mm[5V2FPT0WU
SNU-C2B NFq5VHFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX2yW|FlUUN3ME20MlM1Pjh4IN88US=> NFLISFZUSU6JRWK=
LU-139 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWHJR|UxRTRwM{eyOFch|ryP MXzTRW5ITVJ?
HCC1937 NFXoZmJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWjJR|UxRTRwM{e5PVMh|ryP M1P1UHNCVkeHUh?=
GR-ST NUKyWYlRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkWzTWM2OD12LkSzNVgh|ryP NV3UZ2t{W0GQR1XS
SK-OV-3 Mmm0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRTRwNE[wOFIh|ryP NGG2VnNUSU6JRWK=
A172 MnrtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2npUWlEPTB;ND61OVUzOSEQvF2= Mn;VV2FPT0WU
NB10 NInXZoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHnNfWFKSzVyPUSuOVk4QDFizszN NFvRUpVUSU6JRWK=
P30-OHK M3XO[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHvv[WlKSzVyPUSuPFE6OjlizszN M1PFb3NCVkeHUh?=
KYSE-520 MlXDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTRwOEiwNVgh|ryP MUjTRW5ITVJ?
KNS-62 NYnMXHlvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XmWmlEPTB;ND65NFgxPyEQvF2= Ml\yV2FPT0WU
ES1 NWPEW2wzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTRwOU[wN|Uh|ryP NX;WSWRQW0GQR1XS
KOSC-2 M4X2Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M163eWlEPTB;ND65PVk2QSEQvF2= NIX6WVRUSU6JRWK=
TE-9 NFuwcXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{TBNGlEPTB;NT6wNVUzPSEQvF2= M3;XeHNCVkeHUh?=
NCI-H1838 Mk\iS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkThTWM2OD13LkCyNFc3KM7:TR?= MYfTRW5ITVJ?
KP-N-YN M4fhfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTVwMUK1PVEh|ryP NUHQVYpXW0GQR1XS
ALL-PO MmGxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVfHcGNrUUN3ME21MlIzPTh5IN88US=> NFrvRmlUSU6JRWK=
NOMO-1 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGTYSnBKSzVyPUWuNlMyPzZizszN MnntV2FPT0WU
CGTH-W-1 Mn3RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mor0TWM2OD13LkO4OVg1KM7:TR?= MVXTRW5ITVJ?
ATN-1 NVvNOI86T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEX6TVJKSzVyPUWuOFEyQDRizszN MXfTRW5ITVJ?
AGS MnjvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYS1RoZoUUN3ME21MlQ6PjF6IN88US=> NEHQTVVUSU6JRWK=
SW13 NHnHcmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2XyVGlEPTB;NT61N|I3PSEQvF2= NEPFXGdUSU6JRWK=
SW1990 M2DKUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;DS|dKSzVyPUWuOlIzQDVizszN Mo\PV2FPT0WU
BFTC-905 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2X4NmlEPTB;NT62OVg4QCEQvF2= NXrEU3FuW0GQR1XS
RMG-I MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVHtZVNKUUN3ME21MlY2QTR{IN88US=> Mm\1V2FPT0WU
HD-MY-Z NF3Y[GJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\STWM2OD13Lk[4OVgyKM7:TR?= MnfxV2FPT0WU
HCC1187 Mnf5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HuO2lEPTB;NT63NVA{OiEQvF2= MlnTV2FPT0WU
HCC2998 M1\I[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHFUY5FUUN3ME21MlcyQDd5IN88US=> NWn1RW5XW0GQR1XS
RT-112 NGfwTFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTVwOUCyOFUh|ryP MnPUV2FPT0WU
CAKI-1 M4jEdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHTPdmpKSzVyPU[uNFA6QDVizszN M2S4dnNCVkeHUh?=
HDLM-2 MnfvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4XvVmlEPTB;Nj6wNVQ5QSEQvF2= M4f5fXNCVkeHUh?=
TE-10 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2H6XGlEPTB;Nj6wOFg{OyEQvF2= NUjNOm5RW0GQR1XS
KE-37 MkfVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3rZV2lEPTB;Nj6wO|I2QCEQvF2= MWHTRW5ITVJ?
RCM-1 M2H5Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY\JR|UxRTZwMEe3NVkh|ryP MmXjV2FPT0WU
Mewo M131cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzZZmZKSzVyPU[uNVE1QDdizszN MoPpV2FPT0WU
L-428 MonSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3u3SWlEPTB;Nj6xPFA{PyEQvF2= M3:5RnNCVkeHUh?=
8-MG-BA MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoPWTWM2OD14LkG5N|c6KM7:TR?= NULPfpZ[W0GQR1XS
SK-MEL-28 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1Lzc2lEPTB;Nj6yNFk3PyEQvF2= MVzTRW5ITVJ?
VA-ES-BJ MorES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWnJR|UxRTZwM{C0OlIh|ryP MmLjV2FPT0WU
CAL-120 Mn3yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MknqTWM2OD14LkS1NVYzKM7:TR?= NUO3SYE2W0GQR1XS
GAMG MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1;LV2lEPTB;Nj61NFYzKM7:TR?= M{XQNnNCVkeHUh?=
MSTO-221H MmjKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmfuTWM2OD14LkWxNVch|ryP NWfM[5ZTW0GQR1XS
GCT NFn6UZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYK4NYkyUUN3ME22MlU{Ozh5IN88US=> NYjPS5hYW0GQR1XS
SH-4 M2jBbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jhVWlEPTB;Nj61PFg1PCEQvF2= MYXTRW5ITVJ?
Calu-3 NUXpc5F7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;RXWlEPTB;Nj62OVU3QCEQvF2= MorsV2FPT0WU
SK-HEP-1 MnPUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIfpdIdKSzVyPU[uO|E4PTNizszN MUDTRW5ITVJ?
PFSK-1 NXW2bZV1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\XfopKSzVyPU[uO|M1PzhizszN MWLTRW5ITVJ?
NB12 MkTpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm[3TWM2OD14Lke4OFA1KM7:TR?= NEj0OINUSU6JRWK=
A388 M3\2T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmD5TWM2OD14Lki5OVIh|ryP MX\TRW5ITVJ?
KLE MkKxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWHJR|UxRTZwOU[1NVYh|ryP NH7rXHpUSU6JRWK=
TE-11 Mk\uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH7ST|BKSzVyPUeuNFQ6PjdizszN MWTTRW5ITVJ?
NCI-H2405 NEnhVldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHKyPIdKSzVyPUeuNFg{QTNizszN MXzTRW5ITVJ?
OVCAR-4 M4nwTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn\YTWM2OD15LkG1N|Uh|ryP NX;ld|YxW0GQR1XS
KP-N-YS NF7KRlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHfGeJlKSzVyPUeuNVgyPCEQvF2= M3LBZ3NCVkeHUh?=
NOS-1 NXjhRnJDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRTdwMkW4Nlgh|ryP Mom0V2FPT0WU
DMS-114 M1jYN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkniTWM2OD15LkK5OlMyKM7:TR?= M2iwNXNCVkeHUh?=
NCI-H2030 M{G3OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGXSOmFKSzVyPUeuN|M3QThizszN NWTvSlVtW0GQR1XS
HEL Mo\TS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmDpTWM2OD15LkO3OVIyKM7:TR?= MkHoV2FPT0WU
CAL-62 M4fnUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHPGfHFKSzVyPUeuN|k5PjZizszN MWjTRW5ITVJ?
COLO-668 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3H0e2lEPTB;Nz60N|k3PyEQvF2= NXPxS|B1W0GQR1XS
SKG-IIIa MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1TRTmlEPTB;Nz60OVM5OSEQvF2= NYjrV2J[W0GQR1XS
HCC1419 MmWyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml:2TWM2OD15LkS2PFM{KM7:TR?= M4P3b3NCVkeHUh?=
OS-RC-2 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXO3SHBiUUN3ME23MlQ6QDl2IN88US=> MV3TRW5ITVJ?
COLO-829 NEXsVVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4f2VmlEPTB;Nz62OlQ3OyEQvF2= NIrqd|JUSU6JRWK=
NB13 MlGxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWXOWVNLUUN3ME23MlgzPjZ5IN88US=> NXnY[GpHW0GQR1XS
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D-423MG NGrwO2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlP3TWM2OD1|OD60PFY6KM7:TR?= NHXSN21USU6JRWK=
NCI-H510A MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlW1TWM2OD1|OD62OFUyKM7:TR?= NETyU2xUSU6JRWK=
NMC-G1 MljoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MorHTWM2OD1|OD63NlIzKM7:TR?= MXzTRW5ITVJ?
SW948 M2jBeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGLCNW9KSzVyPUO4Mlg6PDlizszN Mn3UV2FPT0WU
MFM-223 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;2TWM2OD1|OD65OFMyKM7:TR?= MmKxV2FPT0WU
PAN1 NEX0Z|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYTJR|UxRTN6Lkm5NVkh|ryP M3LsVnNCVkeHUh?=
MZ7-mel MojFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTN7LkGxPVkh|ryP NV25XIJNW0GQR1XS
COLO-679 NEfUSpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTN7Lkm3NFgh|ryP Moi5V2FPT0WU
SW872 NFHEcmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTRyLkKwN|Eh|ryP Ml7jV2FPT0WU
HT-1197 MmP4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWHJR|UxRTRyLkK4Olkh|ryP M4LQfnNCVkeHUh?=
ES6 NFXsRmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;aTWM2OD12MD60OVUzKM7:TR?= MmTRV2FPT0WU
SNU-387 NUK4UY57T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\TeFJKSzVyPUSwMlgzOzhizszN M4P0THNCVkeHUh?=
T47D NXL4TmkyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVHr[|VlUUN3ME20NU4xOTl{IN88US=> NGW4WmhUSU6JRWK=
G-402 NYPT[WRPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFnoXZRKSzVyPUSxMlA5ODlizszN NUDBcZRVW0GQR1XS
MZ1-PC MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHZRVNKSzVyPUSxMlgyKM7:TR?= MVvTRW5ITVJ?
SW1417 NGj0e3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUXlb3RUUUN3ME20Nk4{ODNizszN NX3Ub4VnW0GQR1XS
RERF-LC-MS M4PXWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUPJR|UxRTR{LkWwPFch|ryP MXvTRW5ITVJ?
SF539 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVjQWlFOUUN3ME20Nk42PTJ7IN88US=> NXPoXGZIW0GQR1XS
ESS-1 MlX3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVzhVIR7UUN3ME20N{42PTV6IN88US=> MoKxV2FPT0WU
RO82-W-1 M1XhXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{L2N2lEPTB;NEOuOlE2OyEQvF2= NUO5XYl[W0GQR1XS
S-117 NGnYVXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXzUc5pSUUN3ME20N{43QTV6IN88US=> MXnTRW5ITVJ?
SW756 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{\VPWlEPTB;NEOuPFI1OiEQvF2= NHTwVlFUSU6JRWK=
CP66-MEL M131Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVHJR|UxRTR3LkexOFMh|ryP NXjiZVdzW0GQR1XS
H-EMC-SS MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVjid|RFUUN3ME20OU44Pjl|IN88US=> NI\GUm5USU6JRWK=
NCI-H2052 M2\NTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1viPGlEPTB;NEWuPVAzPyEQvF2= MWrTRW5ITVJ?
SW684 MoXBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MofxTWM2OD12Nz6wNFgyKM7:TR?= M1jFcXNCVkeHUh?=
HCC1569 NUPXdG1{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{f6NWlEPTB;NEeuNVY2QCEQvF2= NUTIWHJ2W0GQR1XS
KGN MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\WfGlEPTB;NEeuN|MyOSEQvF2= MXTTRW5ITVJ?
LCLC-103H Mo\ES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NE\SfoxKSzVyPUS3MlQ1ODRizszN M4eyXnNCVkeHUh?=
SW780 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3LwSmlEPTB;NEeuOlM2OSEQvF2= MWPTRW5ITVJ?
NCI-H1703 NVTuSoh1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHnKW5VKSzVyPUS4MlM{PTJizszN M2fqfHNCVkeHUh?=
HCC1395 NVXkT5hUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoDqTWM2OD12OD63OFMzKM7:TR?= M3u4V3NCVkeHUh?=

多くの細胞株試験データを見る場合、クリックしてください

体内試験 Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

お薦めの試験操作(参考用のみ)

細胞試験: [1]
+ 展開
  • 細胞株: NR6, NR6M and NR6W cells
  • 濃度: 0-2 μM
  • 反応時間: 72 hours
  • 実験の流れ: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (参考用のみ)
動物試験:[3]
+ 展開
  • 動物モデル: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • 製剤: 0.5% polysorbate
  • 投薬量: 100 mg/kg
  • 投与方法: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
体内 順序で溶剤を入れること:
5% DMSO+corn oil
2.5mg/mL

* 溶解度検測はSelleck技術部門によって行いますので、文献より提供された溶解度と差異がある可能性がありますが、生産工芸と不同ロット(lot)で起きる正常な現象ですから、ご安心ください。

化学情報

分子量 446.90
化学式

C22H24ClFN4O3

CAS No. 184475-35-2
保管
in solvent
別名 N/A

便利ツール

モル濃度計算器

モル濃度計算器

解決のために必要とされるマス、ボリュームまたは濃度を計算してください。

マス (g) = 濃度 (mol/L) x ボリューム (L) x 分子量 (g/mol)

モル濃度計算器方程式

  • マス
    濃度
    ボリューム
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備することを要求される希釈剤を計算してください. セレック希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 輸入 輸出 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

マス 濃度 ボリューム 分子量

臨床試験

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co., Ltd. September 8, 2016 Phase 1
NCT02856893 Not yet recruiting NSCLC European Organisation for Research and Treatment of Cancer - EORTC|AstraZeneca March 2017 Phase 2
NCT02976116 Recruiting NSCLC Hutchison Medipharma Limited December 2016 Phase 2
NCT02951637 Not yet recruiting Lung Adenocarcinoma Shanghai Chest Hospital|Ruijin Hospital|Changhai Hospital December 2016 Phase 2
NCT02930954 Not yet recruiting Non-small-cell Lung Cancer Caicun Zhou|Tongji University November 2016 Phase 2
NCT02929693 Enrolling by invitation Cancer Shanghai University of Traditional Chinese Medicine|Shanghai Chest Hospital|Shanghai Pulmonary Hospital, Shanghai, China October 2016 Phase 3

技術サポート

ストックの作り方、阻害剤の保管する方法、細胞実験や動物実験に注意すべきな点を全部含めており、製品を取扱う時よくあった質問に対して取扱説明書でお答えいたします。

Handling Instructions

他の質問がある場合は、お気軽くお問合せください。

  • * 必須

よくある質問(FAQ)

  • 問題1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • 回答:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

EGFR信号経路図

EGFR Inhibitors with Unique Features

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細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID