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Geldanamycin

別名：NSC 122750

Geldanamycin is a natural existing HSP90 inhibitor with Kd of 1.2 μM, specifically disrupts glucocorticoid receptor (GR)/HSP association. Geldanamycin attenuates virus infection-induced ALI (acute lung injury)/ARDS (acute respiratory distress syndrome) by reducing the host's inflammatory responses.

Geldanamycin化学構造

CAS No. 30562-34-6

サイズ 価格(税別) 在庫状況
10mM (1mL in DMSO) JPY 57000 国内在庫あり
JPY 25500 国内在庫あり
JPY 40500 国内在庫あり
JPY 100500 国内在庫あり
JPY 598500 国内在庫なし(納期7~10日)

代表番号: 045-509-1970|電子メール：[email protected]
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Geldanamycin関連製品

Antineoplastic and Immunosuppressive Antibiotics阻害剤の選択性比較

Cell Data

Cell Lines Assay Type Concentration Incubation Time 活性情報 PMID
rat neuronal cells Cytotoxicity assay 10 uM 24 hrs Cytotoxicity against rat neuronal cells at 10 uM after 24 hrs 17276679
PP30 Function assay 100 uM 3 hrs Inhibition of human HSP90alpha expressed in yeast PP30 cells co-expressing HSF-lacZ reporter assessed as induction of heat shock response at 100 uM after 3 hrs 21129982
MCF7 Function assay 500 nM 24 hrs Inhibition of Hsp90 in human MCF7 cells assessed as induction of Her2 degradation at 500 nM after 24 hrs by Western blot method 21861487
MCF7 Function assay 500 nM 24 hrs Inhibition of Hsp90 in human MCF7 cells assessed as induction of Raf degradation at 500 nM after 24 hrs by Western blot method 21861487
MCF7 Function assay 500 nM 24 hrs Inhibition of Hsp90 in human MCF7 cells assessed as induction of Akt degradation at 500 nM after 24 hrs by Western blot method 21861487
SKBR3 Function assay 0.5 uM 18 hrs Inhibition of HSP90 in human SKBR3 cells assessed as aggregation of intracellular HER2 at 0.5 uM after 18 hrs by DAPI staining-based immunofluorescence microscopic analysis 23859777
CL1-5 Function assay 2 uM 16 hrs Inhibition of HSP90 in human CL1-5 cells assessed as reduction of total Akt at 2 uM after 16 hrs by Western blotting analysis 24428777
MDA-MB 231 Function assay 0.5 uM 24 hrs Inhibition Hsp90 in human MDA-MB 231 cells assessed as increase in HSP70 protein level at 0.5 uM incubated for 24 hrs by Western blot method 25277067
PC12 Function assay 10 uM 3 to 16 hrs Inhibition of D-aspartate biosynthesis in rat PC12 cells assessed as reduction of intracellular D-aspartate content at 10 uM measured at 3 to 16 hrs by O-phthalaldehyde/N-acetyl-L-cysteine derivatization technique-based HPLC analysis relative to vehicle-t 26642769
PC12 Function assay 10 uM 6 to 16 hrs Inhibition of D-aspartate biosynthesis in rat PC12 cells assessed as reduction of total D-aspartate content at 10 uM measured at 6 to 16 hrs by O-phthalaldehyde/N-acetyl-L-cysteine derivatization technique-based HPLC analysis relative to vehicle-treated c 26642769
PC12 Function assay up to 10 uM 24 hrs Inhibition of D-aspartate biosynthesis in rat PC12 cells assessed as reduction of D-aspartate level in cell culture medium up to 10 uM after 24 hrs by O-phthalaldehyde/N-acetyl-L-cysteine derivatization technique-based HPLC analysis 26642769
A549 Function assay 5 to 10 uM 24 hrs Inhibition of HSP90alpha in human A549 cells assessed as upregulation of HSP90alpha expression at 5 to 10 uM after 24 hrs by Western blot analysis 29486954
A549 Function assay 5 to 10 uM 24 hrs Inhibition of HSP90beta in human A549 cells assessed as upregulation of HSP90beta expression at 5 to 10 uM after 24 hrs by Western blot analysis 29486954
Jurkat Function assay 1 uM Inhibition of to HSP90 in FADD deficient human Jurkat cells assessed as RIP protein degradation at 1 uM by Western blot 18408713
SK-BR-3 Function assay 50 mg/kg Inhibition of oncogene product p185 erbB-2 from human breast cancer cells(SK-BR-3 cells) at 50 mg/kg dose, IC50=0.07μM 7562911
SH-SY5Y Neuroprotection assay 10 nM Neuroprotection against beta-amyloid peptide 1-42-induced toxicity in retinoic acid-differentiated human SH-SY5Y cells assessed as lactate dehydrogenase release at 10 nM 19138859
MCF7 Function assay 0.5 uM Inhibition of Hsp90 in human MCF7 cells assessed as Akt degradation at 0.5 uM by Western blot analysis 21273068
MCF7 Function assay 0.5 uM Inhibition of Hsp90 in human MCF7 cells assessed as Raf degradation at 0.5 uM by Western blot analysis 21273068
MCF7 Function assay 0.5 uM Inhibition of Hsp90 in human MCF7 cells assessed as Hsp90 degradation at 0.5 uM by Western blot analysis 21273068
MCF7 Function assay 0.5 uM Inhibition of Hsp90 in human MCF7 cells assessed as Her2 degradation at 0.5 uM by Western blot analysis 21273068
MDA-MB 231 Function assay 0.5 and 5 uM Inhibition of HSP90 in human MDA-MB 231 cells assessed increase in HSP70 protein levels at 0.5 and 5 uM by Western blot method 25105924
KPL4 Function assay 40 hrs Inhibition of Hsp90 in human KPL4 cells assessed as depletion of Raf-1 level after 40 hrs, MEC=0.33μM 18243703
KPL4 Function assay 40 hrs Inhibition of Hsp90 in human KPL4 cells assessed as depletion of ErbB2 level after 40 hrs, MEC=0.11μM 18243703
P19 Cytotoxicity assay 18 hrs Cytotoxicity against mouse P19 cells after 18 hrs, IC50=0.1μM 17442565
SKBR3 Function assay 24 hrs Inhibition of Hsp90-mediated HER2 degradation in human SKBR3 cells after 24 hrs by Western blot 18816111
SKBR3 Function assay 24 hrs Inhibition of Hsp90-mediated Raf degradation in human SKBR3 cells after 24 hrs by Western blot 18816111
HuH7 Antiviral assay 3 days Antiviral activity against Hepatitis C virus genotype 1b Con1 infected in human HuH7 cells assessed as GAPDH RNA or 18S rRNA level after 3 days by qRT-PCR analysis, EC50=0.00025μM 18936191
HuH7 Antiviral assay 3 days Antiviral activity against Hepatitis C virus genotype 1b Con1 infected in human HuH7 cells assessed as GAPDH RNA or 18S rRNA level after 3 days selected with 40 nM HCV-796 and 800 nM boceprevir by qRT-PCR analysis, EC50=0.0012μM 18936191
SKBR3 Cytotoxicity assay 72 hrs Cytotoxicity against human SKBR3 cells after 72 hrs by celltiter-glo assay, IC50=0.041μM 19405528
A431 Antiproliferative assay 72 hrs Antiproliferative activity against human A431 cells after 72 hrs, IC50=0.2μM 20655237
HCT116 Antiproliferative assay 4 days Antiproliferative activity against human HCT116 cells measured on day 4 by Cell titer-glo assay, EC50=0.03μM 21420297
MCF7 Cytotoxicity assay 72 hrs Cytotoxicity against human MCF7 cells at 72 hrs by MTS assay, IC50=0.053μM 22162786
SKBR3 Antiproliferative assay 72 hrs Antiproliferative activity against estrogen receptor deficient human SKBR3 cells after 72 hrs, IC50=0.0085μM 23648180
MCF7 Antiproliferative assay 72 hrs Antiproliferative activity against human MCF7 cells expressing estrogen receptor after 72 hrs, IC50=0.0098μM 23648180
A549 Cytotoxicity assay 2 days Cytotoxicity against human A549 cells after 2 days by AlamarBlue assay, IC50=0.15μM 23947794
L6 Cytotoxicity assay 72 hrs Cytotoxicity against rat L6 cells after 72 hrs by Alamar Blue assay, IC50=6μM 24580531
Sf9 Function assay 16 hrs Displacement of GM-BODIPY from human full length HSP90 alpha expressed in baculovirus-infected Sf9 cells after 16 hrs by fluorescence polarization assay, IC50=0.074μM 24751441
MDA-kb2 Function assay 18 hrs Inhibition of HSP90 in human MDA-kb2 cells assessed as reduction in glucocorticoid receptor-dependent luciferase expression after 18 hrs by firefly luciferase reporter gene assay, IC50<0.01μM 24984936
MCF7 Antiproliferative assay 72 hrs Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTS/PMS assay, IC50=0.04μM 25075762
MDA-MB-231 Cytotoxicity assay 72 hrs Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay, IC50=0.05μM 25105924
LNCAP Cytotoxicity assay 72 hrs Cytotoxicity against human LNCAP cells after 72 hrs by MTT assay, IC50=0.43μM 25105924
HUVEC Cytotoxicity assay 72 hrs Cytotoxicity against HUVEC cells after 72 hrs by MTT assay, IC50=0.019μM 25277067
A431 Cytotoxicity assay 72 hrs Cytotoxicity against human A431 cells after 72 hrs by MTT assay, IC50=0.04μM 25277067
BGC823 Cytotoxicity assay 72 hrs Cytotoxicity against human BGC823 cells after 72 hrs by MTT assay, IC50=0.04μM 25277067
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, IC50=0.04μM 25277067
MDA-MB-231 Cytotoxicity assay 72 hrs Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay, IC50=0.05μM 25277067
A549 Cytotoxicity assay 72 hrs Cytotoxicity against human A549 cells after 72 hrs by MTT assay, IC50=0.097μM 25277067
HL7702 Cytotoxicity assay 72 hrs Cytotoxicity against human HL7702 cells after 72 hrs by MTT assay, IC50=0.141μM 25277067
SW480 Cytotoxicity assay 72 hrs Cytotoxicity against human SW480 cells after 72 hrs by MTT assay, IC50=0.31μM 25277067
HeLa Cytotoxicity assay 72 hrs Cytotoxicity against human HeLa cells after 72 hrs by MTT assay, IC50=0.798μM 25277067
MCF7 Cytotoxicity assay 72 hrs Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTS/PMS assay, IC50=0.0427μM 25756299
MCF7 Function assay 24 hrs Inhibition of HSP90 in human MCF7 cells assessed as pAkt degradation at 5 times IC50 after 24 hrs by Western blot analysis 25756299
MCF7 Function assay 24 hrs Inhibition of HSP90 in human MCF7 cells assessed as Her2 degradation at 5 times IC50 after 24 hrs by Western blot analysis 25756299
MCF7 Function assay 24 hrs Inhibition of HSP90 in human MCF7 cells assessed as Cdk6 degradation at 5 times IC50 after 24 hrs by Western blot analysis 25756299
MCF7 Function assay 24 hrs Inhibition of HSP90 in human MCF7 cells assessed as Raf degradation at 5 times IC50 after 24 hrs by Western blot analysis 25756299
MCF7 Function assay 24 hrs Change in Cdc37 expression in human MCF7 cells at 5 times IC50 after 24 hrs by Western blot analysis 25756299
MCF7 Function assay 24 hrs Change in p23 expression in human MCF7 cells at 5 times IC50 after 24 hrs by Western blot analysis 25756299
MCF7 Function assay 24 hrs Inhibition of HSP90 in human MCF7 cells assessed as induction of heat shock response-mediated HSP90 production at 5 times IC50 after 24 hrs by Western blot analysis relative to vehicle-treated control 25756299
MCF7 Function assay 24 hrs Inhibition of HSP90 in human MCF7 cells assessed as induction of heat shock response-mediated HSP70 production at 5 times IC50 after 24 hrs by Western blot analysis relative to vehicle-treated control 25756299
MCF7 Function assay 24 hrs Inhibition of HSP90 in human MCF7 cells assessed as induction of heat shock response-mediated HSP27 production at 5 times IC50 after 24 hrs by Western blot analysis relative to vehicle-treated control 25756299
PC12 Function assay 24 hrs Inhibition of D-aspartate biosynthesis in rat PC12 cells assessed as reduction of intracellular D-aspartate level up to 10 uM after 24 hrs by O-phthalaldehyde/N-acetyl-L-cysteine derivatization technique-based HPLC analysis 26642769
A549 Function assay 12 to 24 hrs Inhibition of Hsp90 in human A549 cells assessed as decrease in EGFR levels at five times IC50 value after 12 to 24 hrs by Western blot analysis 26745854
A549 Function assay 12 to 24 hrs Inhibition of Hsp90 in human A549 cells assessed as decrease in Her2 levels at five times IC50 value after 12 to 24 hrs by Western blot analysis 26745854
A549 Function assay 12 to 24 hrs Inhibition of Hsp90 in human A549 cells assessed as decrease in C-Raf levels at five times IC50 value after 12 to 24 hrs by Western blot analysis 26745854
BA/F3 Function assay 48 hrs Inhibition of imatinib-resistant BCR-ABL T315I mutant (unknown origin) expressed in mouse BA/F3 cells assessed as decrease in cell viability after 48 hrs by trypan blue exclusion assay, IC50=1μM 26844689
BA/F3 Function assay 48 hrs Inhibition of imatinib-resistant BCR-ABL E255K mutant (unknown origin) expressed in mouse BA/F3 cells assessed as decrease in cell viability after 48 hrs by trypan blue exclusion assay, IC50=1μM 26844689
BA/F3 Function assay 48 hrs Inhibition of BCR-ABL (unknown origin) expressed in mouse BA/F3 cells assessed as decrease in cell viability after 48 hrs by trypan blue exclusion assay, IC50=5μM 26844689
MDA-MB-231 Antiproliferative assay 72 hrs Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by MTS/PMS assay, Activity=0.06μM 27003516
MDA-MB-231 Antiproliferative assay 72 hrs Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by SRB assay, IC50=0.03μM 27266997
HeLa Antiproliferative assay 72 hrs Antiproliferative activity against human HeLa cells after 72 hrs by SRB assay, IC50=0.06μM 27266997
SNU638 Anticancer assay 72 hrs Anticancer activity against human SNU638 cells measured after 72 hrs by SRB assay, IC50=0.03μM 27283788
Caki1 Anticancer assay 72 hrs Anticancer activity against human Caki1 cells measured after 72 hrs by SRB assay, IC50=0.056μM 27283788
A549 Anticancer assay 72 hrs Anticancer activity against human A549 cells measured after 72 hrs by SRB assay, IC50=0.109μM 27283788
MDA-MB-231 Anticancer assay 72 hrs Anticancer activity against human MDA-MB-231 cells measured after 72 hrs by SRB assay, IC50=0.11μM 27283788
HCT116 Anticancer assay 72 hrs Anticancer activity against human HCT116 cells measured after 72 hrs by SRB assay, IC50=0.15μM 27283788
SKBR3 Antiproliferative assay 1 to 3 days Antiproliferative activity against Her2-overexpressing human SKBR3 cells after 1 to 3 days by MTS assay, GI50=0.43μM 27783977
NCI-H1975 Antiproliferative assay 1 to 3 days Antiproliferative activity against gefitinib-resistant human NCI-H1975 cells after 1 to 3 days by MTS assay, GI50=0.56μM 27783977
RPMI8226 Growth inhibition assay 72 hrs Growth inhibition of human RPMI8226 cells after 72 hrs by MTS/PMS assay, GI50=0.003μM 29057043
MDA-MB-231 Antiproliferative assay 72 hrs Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by SRB assay, IC50=0.06μM 29172085
A549 Antiproliferative assay 72 hrs Antiproliferative activity against human A549 cells after 72 hrs by SRB assay, IC50=0.1μM 29172085
NCI-H1975 Antiproliferative assay 72 hrs Antiproliferative activity against human NCI-H1975 cells after 72 hrs by Cell Titer 96 Aqueous One Solution cell proliferation assay, GI50=0.56μM 29202402
SKBR3 Function assay 24 hrs Inhibition of HSP90 at N-terminus in human SKBR3 cells assessed as induction of Raf1 protein degradation incubated for 24 hrs by Western blot analysis 31591016
SKBR3 Function assay 24 hrs Inhibition of HSP90 at N-terminus in human SKBR3 cells assessed as induction of CDK4 protein degradation incubated for 24 hrs by Western blot analysis 31591016
SKBR3 Function assay 24 hrs Inhibition of HSP90 at N-terminus in human SKBR3 cells assessed as induction of Her2 protein degradation incubated for 24 hrs by Western blot analysis 31591016
SKBR3 Function assay 24 hrs Inhibition of HSP90 at N-terminus in human SKBR3 cells assessed as induction of AKT protein degradation incubated for 24 hrs by Western blot analysis 31591016
Jurkat Function assay Inhibition of TNF-alpha-induced NF-kappaB activation expressed in FADD deficient human Jurkat cells by luciferase reporter gene assay 18408713
HEK293T Function assay Inhibition of TNF-alpha-induced NF-kappaB activation expressed in HEK293T cells by luciferase reporter gene assay 18408713
A549 Growth inhibition assay Growth inhibition of human A549 cells, GI50=0.064μM 18243703
KPL4 Growth inhibition assay Growth inhibition of human KPL4 cells, GI50=0.059μM 18243703
DLD1 Growth inhibition assay Growth inhibition of human DLD1 cells, GI50=0.037μM 18243703
HCT116 Growth inhibition assay Growth inhibition of human HCT116 cells, GI50=0.021μM 18243703
MCF7 Growth inhibition assay Growth inhibition of human MCF7 cells after days by SRB assay, GI50=35.6μM 17869098
LS174T Cytotoxicity assay Cytotoxicity against human LS174T cells by MTS assay, IC50=0.45μM 17034135
HEK293 Function assay Inhibition of HSP90 expressed in HEK293 cells assessed as effect on Akt1:p27 interaction complexes by EYFP and/or YFP Venus fragment based reporter gene assay 16680159
SKBR-3 Function assay Effective concentration for Her-2 degradation in SKBR-3 cells, Concentration=5μM 15713410
MCF-7 Function assay Inhibition of steady-state expression of Raf-1 in MCF-7 breast cancer cells, IC50=0.2μM 10340605
MCF-7 Function assay Inhibition of steady-state expression of ER in MCF-7 breast cancer cells, IC50=0.06μM 10340605
MCF-7 Function assay Inhibition of steady-state expression of HER2 in MCF-7 breast cancer cells, IC50=0.05μM 10340605
SK-BR-3 Function assay In vitro inhibition of p185 erbB-2 oncoprotein in human breast cancer SK-BR-3 cells, IC50=0.07μM 7562912
HT-29 cell Growth inhibition assay Inhibitory concentration against human colorectal carcinoma HT-29 cell lines, IC50=24.5 nM 15658879
K562 cell Growth inhibition assay Inhibitory concentration against human leukemia K562 cell lines, IC50=22.1 nM 15658879
MCF-7 cell Growth inhibition assay Inhibitory concentration against human breast cancer MCF-7 cell lines, IC50=6.5 nM 15658879
SW620 cell Growth inhibition assay Inhibitory concentration against human colorectal carcinoma SW620 cell lines, IC50=6.2 nM 15658879
A2780 cells Proliferation assay Compound was evaluated for antiproliferative activity against human ovarian carcinoma cell line A2780, IC50=3.4 μM 11514145
SH-SY5Y Neuroprotection assay Neuroprotection against beta-amyloid peptide 1-42-induced toxicity in human SH-SY5Y cells assessed as lactate dehydrogenase release, EC50=0.04269μM 19138859
MCF7 Cytotoxicity assay Cytotoxicity against human MCF7 cells by SRB assay, IC50=9.6μM 19560353
SK-BR-3 Antiproliferative assay Antiproliferative activity against human SK-BR-3 cells, IC50=0.0158μM 19896848
MCF7 Antiproliferative assay Antiproliferative activity against human MCF7 cells, IC50=0.0161μM 19896848
HCT116 Antiproliferative assay Antiproliferative activity against human HCT116 cells by luminescence assay, EC50=0.03μM 21605975
U87 Antiproliferative assay Antiproliferative activity against human U87 cells by luminescence assay, EC50=0.089μM 21605975
HCT116 Antiproliferative assay Antiproliferative activity against human HCT116 cells, IC50=0.03μM 21715165
NCI-H1975 Antiproliferative assay Antiproliferative activity against human NCI-H1975 cells, IC50=0.036μM 21715165
U87MG Antiproliferative assay Antiproliferative activity against human U87MG cells, IC50=0.089μM 21715165
HepG2 Cytotoxicity assay Cytotoxicity against human HepG2 cells, IC50=0.37μM 22849774
HepG2 Cytotoxicity assay Cytotoxicity against human HepG2 cells by MTT assay, IC50=0.3μM 23656556
SKBR3 Function assay Inhibition of Hsp90 in human SKBR3 cells, IC50=0.07μM 26844689
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生物活性

製品説明 Geldanamycin is a natural existing HSP90 inhibitor with Kd of 1.2 μM, specifically disrupts glucocorticoid receptor (GR)/HSP association. Geldanamycin attenuates virus infection-induced ALI (acute lung injury)/ARDS (acute respiratory distress syndrome) by reducing the host's inflammatory responses.
Targets
p185 [4]
(SKBr3 cells)		 HSP90 (N-terminal domain) [1]
(Cell-free assay)		 HSP90 [1]
(Cell-free assay)
70 nM 0.78 μM(Kd) 1.2 μM(Kd)
In Vitro
In vitro Geldanamycin binds in the ATP-binding site in the N-terminus domain of Hsp90s (residues 1-220). Geldanamycin inhibits the ATPase activity of Hsp90 in a dose-dependent manner. [1] Geldanamycin causes a dose-dependent G2 arrest and reversible inhibiton o f entry into the S phase in A2780 human ovarian cell line. This inhibition is accompanied by p53 increase and finally demonstrated to be p53 dependent. [2] Geldanamycin causes polyubiquitination and proteasomal degradation of the p185 receptor protein-tyrosin kinase and shows a IC50 with 70 nM. [3, 4] Geldanamycin is a typical anti-tumor reagent, shows a mean GI50 with 0.18 μM against the panel of 60 human tumor cell lines. [5]
Kinase Assay Isothermal Titration Calorimetry (ITC) of Nucelotide Binding
The titration experiments are performed using the MSC system. In each experiment, 16 aliquots of 15 μL of geldanamycin (300 μM in 1% DMSO) are injected into 1.3 mL of protein (31 μM in 20 mMTris-HCl, pH 7.5, 1 mMEDTA) at 25 °C, and the resulting data are fit after subtracting the heats of dilution. Heats of dilution are determined in separate experiments from addition of geldanamycin into buffer and buffer into protein. No evidence for binding of DMSO in the nucleotide binding site is observed. Titration data are fit using a nonlinear least-squares curve-fitting algorithm with three floating variables: stoichiometry, binding constant (Kb) 1/Kd), and change of enthalpy of interaction (ΔH°). Dissociation constants estimated for geldanamycin binding to intact yeast Hsp90 is 1.22 μM, and for binding to Hsp90 N-terminal domain is 0.78 μM. No meaningful heat is observed with binding to the C-terminal fragment.
細胞実験 細胞株 A2780 human ovarian cell line
濃度 0.001-10 μM
反応時間 3 hours
実験の流れ

Exponentially growing cells are treated with Geldanamycin and at various times DNA synthesis is assessed by incorporation of bromodeoxyuridine (BrdUrd) and flow cytometric analysis. No marked difference in total cell number is noted during this time course for treated and untreated cultures. BrdUrd (10 μM) is incorporated over a 4-h incubation period at 37 °C and cells are harvested and fixed in 70% ethanol. After denaturation of the DNA with 2 N HC1, cells are incubated with an anti-BrdUrd mouse monoclonal antibody followed by a fluorescein isothiocyanate (FITC)-linked goat anti-mouse IgG. Cells are stained for 30 minutes at room temperature with propidium iodide and analysed by flow cytometry using a Coulter EPICS Profile Analyzer.
In Vivo
In Vivo Geldanamycin (50 mg//kg) shows 30% inhibition on pl85-associated phosphotyrosine levels in FRE/erbB-2 mice. [6]
動物実験 動物モデル FRE/erbB-2 tumors in nu/nu mice
投与量 50 mg/kg
投与経路 Administered via i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00003969 Completed
Unspecified Adult Solid Tumor Protocol Specific
Cancer Research UK|National Cancer Institute (NCI)
 August 1998 Phase 1

化学情報

分子量 560.64 化学式

C29H40N2O9
CAS No. 30562-34-6 SDF Download Geldanamycin SDFをダウンロードする
Smiles CC1CC(C(C(C=C(C(C(C=CC=C(C(=O)NC2=CC(=O)C(=C(C1)C2=O)OC)C)OC)OC(=O)N)C)C)O)OC
保管

In vitro
Batch:

DMSO : 100 mg/mL ( (178.36 mM)； Warmed with 50℃ water bath; 吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : Insoluble

Ethanol : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量 濃度 体積 分子量

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

mg/kg g μL 匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH2O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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