Obacunone (AI3-37934)

別名：CCRIS 8657

製品コード：S3784 純度：99.85%

Obacunone (AI3-37934, CCRIS 8657), a natural compound present in citrus fruits, has been demonstrated for various biological activities including anti-cancer and anti-inflammatory properties. It significantly inhibits aromatase activity in an in vitro enzyme assay with an IC50 value of 28.04 μM; also a novel activator of Nrf2.

CAS No. 751-03-1

サイズ	価格(税別)	在庫状況
5mg	JPY 23100	国内在庫あり
25mg	JPY 66300	国内在庫あり

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

文献中Selleckの製品使用例（2）

製品安全説明書

現在のバッチを見る：純度： 99.85%

99.85

Obacunone (AI3-37934)関連製品

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シグナル伝達経路

Aromataseシグナル伝達経路

Aromatase阻害剤の選択性比較

生物活性

製品説明

Targets

Nrf2 ^[2]	Aromatase ^[1] (Cell-free assay)
	28.4 μM

In Vitro
In vitro	Obacunone is strongly inhibited MCF-7 cell proliferation without affecting non-malignant breast cells. Treatment with obacunone increases apoptosis by upregulating expression of the pro-apoptotic protein Bax and down-regulating the anti-apoptotic protein Bcl2, as well as inducing G1 cell cycle arrest. In addition, obacunone significantly inhibits aromatase activity in an in vitro enzyme assay. Exposure of MCF-7 breast cancer cells to obacunone down-regulates expression of inflammatory molecules including nuclear factor-kappa B (NF-kB) and cyclooxygenase-2 (COX-2). Obacunone inhibits COX-2 and NF-kB by activation of the p38 mitogen-activated protein kinase (MAPK). Obacunone is reported as a glutathione S-transferase (GST) enzyme inducer as well as a neuroprotective agent acting by induction of heme oxygenase-1 via the p38 MAPK pathway^[1]. Obacunone is a novel activator of Nrf2 by decreasing Nrf2 ubiquitination and increasing its stability. It can effectively protect cells from oxidative stress by activating the Nrf2 pathway. Obacunone could induce the expression of the ARE-dependent luciferase gene in a dose-dependent manner^[2].
細胞実験	細胞株	Human breast cancer (MCF-7) and non-malignant immortalized breast epithelial (MCF-12F) cell lines
	濃度	12.5, 25, 50,100 and 200 μM
	反応時間	24, 48, and 72 h
	実験の流れ	The MCF-7 cells are cultured in DMEM medium containing 10% (v/v) fetal bovine serum, 200 U/mL penicillin G, and 200 mg/mL streptomycin. The MCF-12F cells are cultured in a 1:1 mixture of DMEM and Ham's F12 medium with 20 ng/mL of epidermal growth factor (EGF),100 ng/mL cholera toxin, 0.01 mg/mL insulin, 500 ng/mL hydrocortisone, and 5% (v/v) chelextreated horse serum, and then the cells are incubated at 37℃ with 5% CO2. To evaluate the anti-proliferate effects of obacunone, viability of the MCF-7 and MCF-12F cells are measured using the tetrazolium MTT reduction assay. The MCF-7 or MCF-12F cells (1×10⁴/well) are seeded into a 96-well plate and allowed to adhere overnight. Cells are treated with different concentrations (12.5, 25, 50,100 and 200 μM) of either obacunone or OG and incubated for 24, 48, and 72 h. Tamoxifen, a well-known anti-estrogen drug used for breast cancer therapy, is used as a positive control. After the various time points, 10 ml of the MTT (5 mg/mL) reagent are added to each well and incubated for 2h at 37℃. After 2h incubation, the medium is removed and 200 ml of DMSO is added to dissolve the purple formazan. The absorbance is measured by an ELISA microplate reader at 570 nm.

In Vivo
In Vivo	The systemic administration of obacunone strongly inhibits bleomycin-induced lung fibrosis in mice. Obacunone can inhibit cancer proliferation and has some therapeutic effects on cardiovascular diseases^[2]. In vitro and animal studies suggests that obacunone and a few other limonoids may have potential anticarcinogenic activity against certain types of cancers. A 500-mg/kg (of body weight) dose of obacunone per day is well tolerated and does not have adverse effects in rats, indicating a low toxicity^[3].
動物実験	動物モデル	Eight-week-old B6 mice
	投与量	10 mg/kg
	投与経路	i.p.

参考
[1] Kim J, et al. Biochimie. 2014, 105:36-44. [2] Shengmei Xu, et al. Protein Cell. 2016, 7(9): 684–688. [3] Amit Vikram, et al. Appl Environ Microbiol. 2012, 78(19):7012-22.

参考

化学情報

分子量	454.51	化学式	C₂₆H₃₀O₇
CAS No.	751-03-1	SDF	Download Obacunone (AI3-37934) SDFをダウンロードする
Smiles	CC1(C2CC(=O)C3(C(C2(C=CC(=O)O1)C)CCC4(C35C(O5)C(=O)OC4C6=COC=C6)C)C)C
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1个月-20°Cin solvent

In vitro
Batch:

DMSO : 90 mg/mL ( (198.01 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

C1=C0/X	C1:	LOG(C1):
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C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
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C8=C7/X	C8:	LOG(C8):