Uric Acid

別名：2,6,8-Trioxypurine, 2,6,8-Trihydroxypurine, 2,6,8-Trioxopurine

製品コード：S3955 純度：99.99%

Uric Acid, a normal component of urine, is a product of the metabolic breakdown of purine nucleotides.

CAS No. 69-93-2

サイズ	価格(税別)	在庫状況
25mg	JPY 16300	国内在庫あり
1g	JPY 111100	国内在庫なし(納期7~10日)

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

製品安全説明書

現在のバッチを見る： S395501 0.5MNAOH] 3.02 mg/mL] false] DMSO] 0.01 mg/mL] false] Water] Insoluble] false 純度： 99.99%

99.99

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関連化合物ライブラリー	FDA-approved Drug Library Natural Product Library Bioactive Compound Library-I Bioactive Compound Library-Ⅱ Express-Pick Library	もっと見る

生物活性

製品説明	Uric Acid, a normal component of urine, is a product of the metabolic breakdown of purine nucleotides.

In Vitro
In vitro	Uric acid activates NFκB in a variety of cell culture models including proximal tubular cells. Uric acid suppresses 1-α hydroxylase mRNA and protein expression in dose dependent and time dependent manner^[1].
細胞実験	細胞株	HK2 cells
	濃度	2.5, 5.0, 7.5, and 10 mg/dL
	反応時間	1, 2, 4, 8, 16, and 24 hours
	実験の流れ	Cells are cultured in Keratinocyte-SFM basal media, supplemented with Bovine Pituitary Extract (20-30 μg/mL) and recombinant Epidermal Growth Factor (0.1-0.2 ng/mL), 5% Fetal Bovine Serum, 100 U/ml penicillin, and 10 g/ml streptomycin. Cells are cultured at 37°C in 95% air-5% CO2 until they were 90% confluent, and then allowed to differentiate for 5-7 days. After serum starvation for 24 hours, cells are stimulated with uric acid. For the dose response experiments, uric acid is given at 2.5, 5.0, 7.5, and 10 mg/dL and the cells are collected after 24 hours of treatment. Uric acid is used at 10 mg/dL for the time course experiments and the cells are collected at: 1, 2, 4, 8, 16, and 24 hours. To prevent uric acid crystal formation in the media, uric acid is dissolved in prewarmed media, and kept at 37°C for a minimum of 30 minutes prior to application in cell culture.

In Vivo
In Vivo	Uric acid is synthesized mainly in the liver, intestines and the vascular endothelium as the end product of an exogenous pool of purines, and endogenously from damaged, dying and dead cells, whereby nucleic acids, adenine and guanine, are degraded into uric acid. Uric acid is a strong reactive oxygen species (ROS) and peroxynitrite scavenger and antioxidant. Uric acid may exert fundamental roles in tissue healing via initiating the inflammatory process that is necessary for tissue repair, scavenging oxygen free radicals, and mobilizing progenitor endothelial cells^[2].

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
臨床試験 (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05298891	Not yet recruiting	Acromegaly	Azienda Ospedaliero Universitaria Maggiore della Carita	September 1 2024	Not Applicable
NCT06126315	Not yet recruiting	Amyotrophic Lateral Sclerosis	Mario Negri Institute for Pharmacological Research\|University of Sydney\|FightMND	January 2024	Phase 2\|Phase 3
NCT06149078	Not yet recruiting	Knee Osteoarthritis	Sohag University	December 10 2023	--

参考
[1] Chen W, et al. Metabolism. 2014, 63(1):150-60. [2] Rashika El Ridi, et al. J Adv Res. 2017, 8(5): 487-493.

参考

化学情報

分子量	168.11	化学式	C₅H₄N₄O₃
CAS No.	69-93-2	SDF	Download Uric Acid SDFをダウンロードする
Smiles	C12=C(NC(=O)N1)NC(=O)NC2=O
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1个月-20°Cin solvent

In vitro
Batch:

0.5MNAOH : 3.02 mg/mL

DMSO : 0.01 mg/mL ( (0.05 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

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