EIPA

製品コードS9849 バッチS984901

印刷

化学情報

Synonyms

L593754; MH 12-43

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₁₁H₁₈ClN₇O

分子量

299.76

CAS No.

1154-25-2

Solubility (25°C)*

体外

DMSO

60 mg/mL (200.16 mM)

Ethanol

30 mg/mL (100.08 mM)

Water

2 mg/mL (6.67 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	5.500mg/ml (18.35mM)	Taking the 1 mL working solution as an example, add 50 μL of 110 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	5.500mg/ml (18.35mM)	Taking the 1 mL working solution as an example, add 50 μL of 110 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	EIPA (L593754; MH 12-43) acts as an inhibitor of macropinocytosis and sodium-hydrogen exchangers(NHE) (IC50=0.033μg/mL). EIPA blocks the activity of Na(+)/H(+) exchanger, which are plasma membrane proteins implicated in all forms of macropinocytosis.
in vitro	This compound rapidly and reversibly block Ca²⁺-activated TRPP3 channel activation, with IC₅₀s of 10.5 μM.^[2]
in vivo	EIPA downregulates endothelial cell activation of NF-kappaB and VCAM-1 expression and attenuates the early inflammatory stages of atherogenesis and stent intimal formation^[3].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Xenopus laevis oocyte
	濃度	100 μM
	反応時間	30 min
	実験の流れ	The uptake of radioactive ₄₅Ca₂₊ mediated by TRPP3 channel is tested using the standard NaCl-containing solution, plus 1 mM nonradiolabeled CaCl2 and radiolabeled ₄₅Ca₂₊ in the presence and absence of 100 μM EIPA. Control uptake level is obtained using H2O-injected oocytes.
動物実験	動物モデル	Murine Model of Atherogenesis (apoE-/- mice)
	投薬量	3 mg/kg
	投与方法	s.c.

参考

https://pubmed.ncbi.nlm.nih.gov/24184990/
https://pubmed.ncbi.nlm.nih.gov/17804601/
https://pubmed.ncbi.nlm.nih.gov/15505102/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Prophylactic supplementation with biogenic selenium nanoparticles mitigated intestinal barrier oxidative damage through suppressing epithelial-immune crosstalk with gut-on-a-chip [ J Adv Res, 2025, S2090-1232(25)00267-X]	PubMed: 40262719
An anti-CD19-exosome delivery system navigates the blood-brain barrier for targeting of central nervous system lymphoma [ J Nanobiotechnology, 2025, 23(1):173]	PubMed: 40045315
Amniotic epithelial Cell microvesicles uptake inhibits PBMCs and Jurkat cells activation by inducing mitochondria-dependent apoptosis [ iScience, 2025, 28(2):111830]	PubMed: 39967871
Invasion of pancreatic ductal epithelial cells by Enterococcus faecalis is mediated by fibronectin and enterococcal fibronectin-binding protein A [ Sci Rep, 2025, 15(1):2585]	PubMed: 39833342
WNT Oncogenic Transcription Requires MYC Suppression of Lysosomal Activity and EPCAM Stabilization in Gastric Tumors [ Gastroenterology, 2024, S0016-5085(24)05182-5]	PubMed: 38971196
Porcine deltacoronavirus enters ST cells by clathrin-mediated endocytosis and does not require Rab5, Rab7, or Rab11 [ Microbiol Spectr, 2023, 10.1128/spectrum.02553-23]	PubMed: 37962380
In Vivo Functional Assay in Fish Gills: Exploring Branchial Acid-Excreting Mechanisms in Zebrafish [ Int J Mol Sci, 2022, 23(8)4419]	PubMed: 35457237
Porcine Deltacoronavirus (PDCoV) Entry into PK-15 Cells by Caveolae-Mediated Endocytosis [ Viruses, 2022, 14(3)496]	PubMed: 35336903

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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