7,12-Dimethylbenz[a]anthracene (DMBA)

製品コードE1022 バッチE102204

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₂₀H₁₆

分子量

256.34

CAS No.

57-97-6

Solubility (25°C)*

体外

DMSO

14 mg/mL (54.61 mM)

Ethanol

4 mg/mL (15.6 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.700mg/ml (2.73mM)	Taking the 1 mL working solution as an example, add 50 μL of 14 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.700mg/ml (2.73mM)	Taking the 1 mL working solution as an example, add 50 μL of 14 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	7,12-Dimethylbenz[a]anthracene (DMBA) is an immunosuppressor as well as a potent organ-specific carcinogen.
in vitro	In splenocytes, which is isolated from mice, lymphocyte function is completely suppressed by the treatment with 50 μM 7,12-Dimethylbenz[a]anthracene (DMBA). The susceptibility of splenocytes isolated from mEH-null mice is lower than from wildtype mice on the immunosuppression activity of this compound.^[1]
in vivo	The metabolic activation of 7,12-Dimethylbenz[a]anthracene (DMBA) in the spleen plays a key role in the splenic immunotoxicity, and mEH-null mice are more resistant to its cytotoxicity as well as immunosuppression.^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Splenocytes
	濃度	10 μM, 20 μM, 50 μM
	反応時間	--
	実験の流れ	Spleens are harvested and placed in a sterile phosphatebuffered saline. Cell suspensions are prepared by gentle dissociation using glass slides. Debris is separated from cells by filtration and cell suspensions are centrifuged at 250 x g for 5 min. Cells are seeded in a volume of 100 µl in 96-well plates. Mitogens were added in a volume of 10 µl at final concentrations of 25 µg/ml for LPS and 5 µg/ml for PHA, and then the cells were incubated in 5% CO2 at 37°C for 3 days. The cells were harvested onto filter strips using a harvester and [3 H]-thymidine incorporation was determined by liquid scintillation. For mitogen-induced lymphocyte proliferation assays after treatment with 7,12-Dimethylbenz[a]anthracene (DMBA) in vitro, cell preparations from untreated mice were simultaneously exposed to mitogen (LPS) and this compound.
動物実験	動物モデル	mEH-null mice
	投薬量	30 mg/kg, 100 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/11488430/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Diet induced mitochondrial DNA replication instability in Rad51c mutant mice drives sex-bias in anemia of inflammation [ bioRxiv, 2024, 2024.09.21.613572]	PubMed: 39345482

Diet induced mitochondrial DNA replication instability in Rad51c mutant mice drives sex-bias in anemia of inflammation [ bioRxiv, 2024, 2024.09.21.613572]

PubMed: 39345482

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。