Calcitriol

製品コードS1466 バッチS146605

印刷

化学情報

Synonyms

RO215535, Topitriol, 1,25-Dihydroxyvitamin D3

Storage
(From the date of receipt)

1 year -80°C powder（in dark and seal）

化学式

C₂₇H₄₄O₃

分子量

416.64

CAS No.

32222-06-3

Solubility (25°C)*

体外

DMSO

83 mg/mL (199.21 mM)

Ethanol

83 mg/mL (199.21 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Calcitriol is a nonselective vitamin D receptor activator/agonist(VDRA), exhibiting a 10-fold higher vitamin D receptor (VDR) binding affinity(IC50=0.4 nM) than the selective VDRA paricalcitol.Solutions are unstable and should be fresh-prepared.
in vitro	Calcitriol is a potent inhibitor of PHA-induced lymphocyte proliferation, achieving 70% inhibition of tritiated thymidine incorporation after 72 h in culture. This compound suppresses interleukin-2 (IL-2) production by PHA-stimulated peripheral blood mononuclear cells in a concentration-dependent fashion. ^[1] It increases the concentration of intracellular calcium ([Ca2+]i) within 5 s by mobilizing calcium from the endoplasmic reticulum and the formation of inositol 1,4, 5-trisphosphate and diacylglycerol. ^[2] This chemical can inhibit the proliferation and promote the differentiation of human prostate adenocarcinoma cells. It causes a selective decrease in the secreted levels of type IV collagenases (MMP-2 and MMP-9). ^[3] It has antiproliferative activity in squamous cell carcinoma and prostatic adenocarcinoma and enhances the antitumor activity of platinum-based agents. This compound prior to paclitaxel significantly reduces clonogenic survival compared with either agent alone in the murine squamous cell carcinoma and PC-3 cells. ^[4] It is a potent anti-proliferative agent in a wide variety of malignant cell types. Its effects are associated with an increase in G0/G1 arrest, induction of apoptosis and differentiation, modulation of expression of growth factor receptors. This agent potentiates the antitumor effects of many cytotoxic agents and inhibits motility and invasiveness of tumor cells and formation of new blood vessels. ^[5]

製品説明

Calcitriol is a nonselective vitamin D receptor activator/agonist(VDRA), exhibiting a 10-fold higher vitamin D receptor (VDR) binding affinity(IC50=0.4 nM) than the selective VDRA paricalcitol.Solutions are unstable and should be fresh-prepared.

in vitro

Calcitriol is a potent inhibitor of PHA-induced lymphocyte proliferation, achieving 70% inhibition of tritiated thymidine incorporation after 72 h in culture. This compound suppresses interleukin-2 (IL-2) production by PHA-stimulated peripheral blood mononuclear cells in a concentration-dependent fashion. ^[1]

It increases the concentration of intracellular calcium ([Ca2+]i) within 5 s by mobilizing calcium from the endoplasmic reticulum and the formation of inositol 1,4, 5-trisphosphate and diacylglycerol. ^[2]

This chemical can inhibit the proliferation and promote the differentiation of human prostate adenocarcinoma cells. It causes a selective decrease in the secreted levels of type IV collagenases (MMP-2 and MMP-9). ^[3]

It has antiproliferative activity in squamous cell carcinoma and prostatic adenocarcinoma and enhances the antitumor activity of platinum-based agents. This compound prior to paclitaxel significantly reduces clonogenic survival compared with either agent alone in the murine squamous cell carcinoma and PC-3 cells. ^[4]

It is a potent anti-proliferative agent in a wide variety of malignant cell types. Its effects are associated with an increase in G0/G1 arrest, induction of apoptosis and differentiation, modulation of expression of growth factor receptors. This agent potentiates the antitumor effects of many cytotoxic agents and inhibits motility and invasiveness of tumor cells and formation of new blood vessels. ^[5]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	CLL cells
	濃度	100 nM
	反応時間	24 h
	実験の流れ	CLL cells were treated with DMSO (vehicle control) or calcitriol (100 nM). Cells were harvested at the indicated time points with mild trypsinization
動物実験	動物モデル	Male Sprague–Dawley rats
	投薬量	200 ng/kg
	投与方法	p.o.

参考

https://pubmed.ncbi.nlm.nih.gov/6332829/
https://pubmed.ncbi.nlm.nih.gov/9115251/
https://pubmed.ncbi.nlm.nih.gov/9298581/

https://pubmed.ncbi.nlm.nih.gov/11309356/
https://pubmed.ncbi.nlm.nih.gov/15225831/

+ 展开

カスタマーフィードバック

: Data independently produced by , , Mireia Vila Gasull University of Porto. 2011;Mireia Vila Gasull

: Data from [Data independently produced by , , Mol Cell Endocrinol, 2018, 470:1-13]

: Data from [Data independently produced by , , Inflammation, 2017, 40(6):1884-1893]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Vitamin D impedes eosinophil chemotaxis via inhibiting glycolysis-induced CCL26 expression in eosinophilic chronic rhinosinusitis with nasal polyps [ Cell Commun Signal, 2025, 23(1):104]	PubMed: 39985085
p53 Deficiency in Colon Cancer Cells Promotes Tumor Progression Through the Modulation of Meflin in Fibroblasts [ Cancer Sci, 2025, 116(7):1871-1882]	PubMed: 40241262
Targeting ERβ1-Positive Triple-Negative Breast Cancer: Molecular Effects of Calcitriol and 17β-Estradiol [ Cureus, 2025, 17(4):e82934]	PubMed: 40416168
Vitamin D can mitigate sepsis-associated neurodegeneration by inhibiting exogenous histone-induced pyroptosis and ferroptosis: Implications for brain protection and cognitive preservation [ Brain Behav Immun, 2024, 124:40-54]	PubMed: 39566666
VDR regulates mitochondrial function as a protective mechanism against renal tubular cell injury in diabetic rats [ Redox Biol, 2024, 70:103062]	PubMed: 38320454
Protective Effects of Vitamin D on Proteoglycans of Human Articular Chondrocytes through TGF-β1 Signaling [ Nutrients, 2024, 16(17)2991]	PubMed: 39275306
1,25‑Dihydroxyvitamin D3 mitigates the adipogenesis induced by bisphenol A in 3T3-L1 and hAMSC through miR-27-3p regulation [ Int J Obes (Lond), 2024, ]	PubMed: 39256615
Calcitriol impairs the secretion of IL-4 and IL-13 in Th2 cells via modulating VDR-Gata3-Gfi1 axis [ bioRxiv, 2024, ]	PubMed: none
In Vivo and In Vitro Evidence for an Interplay between the Glucocorticoid Receptor and the Vitamin D Receptor Signaling [ Cells, 2023, 12(18)2291]	PubMed: 37759513
In Vivo and In Vitro Evidence for an Interplay between the Glucocorticoid Receptor and the Vitamin D Receptor Signaling [ Cells, 2023, 12(18)2291]	PubMed: 37759513

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。