Atomoxetine HCl

製品コードS3175 バッチS317501

印刷

化学情報

Chemical Structure Synonyms LY 139603 HCl Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C17H21NO.HCl
分子量 291.82 CAS No. 82248-59-7
Solubility (25°C)* 体外 DMSO 58 mg/mL (198.75 mM)
Ethanol 37 mg/mL (126.79 mM)
Water 2 mg/mL (6.85 mM)
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 2.9mg/ml (9.94mM) Taking the 1 mL working solution as an example, add 50 μL of 58 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution
5% DMSO 95% Corn oil

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 0.41mg/ml (1.40mM) Taking the 1 mL working solution as an example, add 50 μL of 8.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Atomoxetine (LY 139603) is a selective norepinephrine (NE) transporter inhibitor with Ki of 5 nM, with 15- and 290-fold lower affinity for human 5-HT and DA transporters.
in vitro Atomoxetine is a selective norepinephrine reuptake inhibitor with Ki of 5 nM, compared with 77 and 1451 nM for binding to serotonin and dopamine transporters. [1]
in vivo In microdialysis studies, atomoxetine increases extracellular (EX) levels of NE in prefrontal cortex (PFC) 3-fold, but does not alter 5-HTEX levels. Atomoxetine also increases DAEX concentrations in PFC 3-fold, but does not alter DAEX in striatum or nucleus accumbens. Atomoxetine increases Fos by 3.7-fold in PFC, but not in the striatum or nucleus accumbens. [1] Atomoxetine selectively inhibits the presynaptic uptake of norepinephrine in adrenergic neurons in animals, and has activity in animal models of depression. [2][3]

プロトコル(参考用のみ)

キナーゼアッセイ Binding affinity
Membranes from MDCK and HEK293 cell lines are transfected with human 5-HT, NE and DA transporters, respectively. The radioligands for 5-HT, NE and DA human transporters are [3H]-paroxetine, [3H]-nisoxetine and [3H]-WIN35,428, respectively. After incubation at 37 °C for 40 min for the 5-HT transporter and 25 °C for 30 min for NE and DA transporters, the binding are terminated by rapid vacuum filtration over Whatman GF/B filters and the filters are washed four times with cold Tris-HCl buffer. The filters are then placed in vials containing liquid scintillation fluid and radioactivity are measured by liquid scintillation spectrometr
動物実験 動物モデル rat
投薬量 3 mg/kg
投与方法 i.p.

参考

  • https://pubmed.ncbi.nlm.nih.gov/12431845/
  • https://pubmed.ncbi.nlm.nih.gov/3965689/
  • https://pubmed.ncbi.nlm.nih.gov/6123593/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Atomoxetine suppresses radioresistance in glioblastoma via circATIC/miR-520d-5p/Notch2-Hey1 axis [ Cell Commun Signal, 2024, 22(1):532] PubMed: 39501373

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。