BV-6

製品コードS7597 バッチS759703

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₇₀H₉₆N₁₀O₈.xCF₃COOH

分子量

1205.57

CAS No.

1001600-56-1(freebase)

Solubility (25°C)*

体外

DMSO

100 mg/mL (82.94 mM)

Water

100 mg/mL (82.94 mM)

Ethanol (warmed with 50ºC water bath)

100 mg/mL (82.94 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	BV-6 is a SMAC mimetic, dual cIAP and XIAP inhibitor.
in vitro	BV6 inhibits the cell viability of HCC193 NSCLC cells with IC50 of 7.2 μM, induces apoptosis in both HCC193 and H460 cell lines, and also significantly enhances the radiosensitivity of these cell lines through activation of cleaved caspase-8 and cleaved caspase-9, respectively. ^[1] In immature dendritic cells, this compound treatment results in moderate activation of the classical NF-kB pathway. ^[2] Furthermore, it increases CIK cell-mediated lysis of hematological (H9, THP-1, and Tanoue) and solid malignancies (RH1, RH30, and TE671). This chemical also enhances apoptosis of peripheral blood mononuclear cells and most notably has an inhibitory effect on immune cells limiting their cytotoxic potential. ^[3]
in vivo	BV6 is an IAP antagonist.

製品説明

BV-6 is a SMAC mimetic, dual cIAP and XIAP inhibitor.

in vitro

BV6 inhibits the cell viability of HCC193 NSCLC cells with IC50 of 7.2 μM, induces apoptosis in both HCC193 and H460 cell lines, and also significantly enhances the radiosensitivity of these cell lines through activation of cleaved caspase-8 and cleaved caspase-9, respectively. ^[1]

In immature dendritic cells, this compound treatment results in moderate activation of the classical NF-kB pathway. ^[2]

Furthermore, it increases CIK cell-mediated lysis of hematological (H9, THP-1, and Tanoue) and solid malignancies (RH1, RH30, and TE671). This chemical also enhances apoptosis of peripheral blood mononuclear cells and most notably has an inhibitory effect on immune cells limiting their cytotoxic potential. ^[3]

in vivo

BV6 is an IAP antagonist.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HCC193 and H460 cells
	濃度	~30 μM
	反応時間	~48 hours
	実験の流れ	Cell viability is measured using the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay kit. 5000 cells/well are seeded into 96-well plates in triplicate. Following adhesion of cells to the wells, increasing concentrations of BV6 are added into different wells. Control groups are exposed to the same concentration of DMSO. The final concentrations of 333 μg/mL MTS and 25 μM PMS are added to each well 24 hours later. After two hours incubation at 37 °C in humidified 5% CO₂, plates are read at the absorbance of 490-nm on a microplate reader. Relative cell viability of an individual sample is calculated by normalizing their absorbance to that of the corresponding control. IC50 values are calculated using Prism 5.01. For the TNFα neutralizing antibody assay, cells are exposed to 1 and 5 μM of this compound and the assay is performed 24 hours later. Plates are read at the absorbance of 490-nm on a microplate reader
動物実験	動物モデル	Female BALB/c mice
	投薬量	10 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/21760551/
https://pubmed.ncbi.nlm.nih.gov/21738708/
https://pubmed.ncbi.nlm.nih.gov/25101252/

https://pubmed.ncbi.nlm.nih.gov/25376458/

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カスタマーフィードバック

: , , FASEB J, 2017, 31(11):4707-4719

: Data from [Data independently produced by , , Clin Cancer Res, 2018, doi:10.1158/1078-0432.CCR-18-1548]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Ferroptosis-activating metabolite acrolein antagonizes necroptosis and anti-cancer therapeutics [ Nat Commun, 2025, 16(1):4919]	PubMed: 40425585
Shigella type-III secretion system effectors counteract the induction of host inflammation and cell death [ EMBO J, 2025, 10.1038/s44318-025-00561-7]	PubMed: 40931196
The cancer-associated SF3B1K700E spliceosome mutation confers enhanced sensitivity to BV-6-induced cytotoxicity [ Cell Death Dis, 2025, 16(1):476]	PubMed: 40595498
Anti-inflammatory mechanism of the MLKL inhibitor necrosulfonamide in LPS- or poly(I:C)-induced neuroinflammation and necroptosis [ Biochem Pharmacol, 2025, 239:117021]	PubMed: 40473224
Adenosine triphosphate-binding pocket inhibitor for mixed lineage kinase domain-like protein attenuated alcoholic liver disease via necroptosis-independent pathway [ World J Gastroenterol, 2025, 31(6):96782]	PubMed: 39958438
Linear ubiquitination prevents lipodystrophy and obesity-associated metabolic syndrome [ Sci Adv, 2025, 11(38):eadw2539]	PubMed: 40961178
Lymph node and tumor-associated PD-L1+ macrophages antagonize dendritic cell vaccines by suppressing CD8+ T cells [ Cell Rep Med, 2024, 5(1):101377]	PubMed: 38232703
RIPK1 protects naive and regulatory T cells from TNFR1-induced apoptosis [ Cell Death Differ, 2024, 10.1038/s41418-024-01301-w]	PubMed: 38734851
Starvation-induced phosphorylation activates gasdermin A to initiate pyroptosis [ Cell Rep, 2024, 43(9):114728]	PubMed: 39264808
The cell cycle inhibitor p21CIP1 is essential for irinotecan-induced senescence and plays a decisive role in re-sensitization of temozolomide-resistant glioblastoma cells to irinotecan [ Biomed Pharmacother, 2024, 181:117634]	PubMed: 39489121

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。