Forskolin (Colforsin)

製品コードS2449 バッチS244909

化学情報

	Synonyms	HL 362, Coleonol	Storage (From the date of receipt)	3 years -20°C(in the dark) powder 1 year -80°C(in the dark) in solvent
	化学式	C₂₂H₃₄O₇
	分子量	410.5	CAS No.	66575-29-9
Solubility (25°C)*	体外	DMSO	82 mg/mL (199.75 mM)
		Ethanol	82 mg/mL (199.75 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	コルホルチン (Forskolin (Colforsin)) は、広範な細胞腫における真核生物のアデニル酸シクラーゼ (adenylate cyclase, AC) の遍在する活性化因子であり、細胞生理学の研究において cAMP のレベルを上げるために一般的に使用されています。ホルスコリン (Forskolin) は、PXR と FXR も活性化します。ホルスコリンはオートファジー (autophagy) を刺激します。
in vitro	Forskolin (Colforsin) increases cAMP levels in preparations of membranes, cells, or tissues. It not only activates AC but also interacts with certain other proteins, including glucose transporters and ion channels. This compound is able to promote activation of nine different transmembrane iso-forms of AC, albeit with somewhat less efficacy for AC9, which could be used to provides a means to identify and quantify high-affinity binding sites, i.e., G-proteins (G_s)–AC complexes. Activation of _s by GPCRs contributes to Forskolin-stimulated cAMP generation in cells because of _s-Forskolin potentiation of AC activity. ^[1] It stimulates adenylate cyclase activity without interacting with cell surface receptors. Its potentiation of cAMP in turn inhibits basophil and mast cell degranulation and histamine release, lowers blood pressure and intraocular pressure, inhibits platelet aggregation, promotes vasodilation, bronchodilation, and thyroid hormone secretion, and stimulates lipolysis in fat cells. Forskolin inhibits the binding of platelet-activating factor (PAF), independently of cAMP formation, which may be a result of its direct effect on PAF or via interference with PAF binding to receptor sites. It also appears to have an effect on several membrane transport proteins, and inhibits glucose transport in erythrocytes, adipocytes, platelets, and other cells. This compound is used to treat with glaucoma. ^[2]
in vivo	Forskolin (Colforsin, HL 362, Coleonol) is a ubiquitous activator of eukaryotic adenylyl cyclase (AC) in a wide variety of cell types, commonly used to raise levels of cAMP. It also activates PXR and FXR activity and stimulates autophagy.^[4]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	hMPCs or cynMPCs
	濃度	20 μM
	反応時間	2 h
	実験の流れ	For synchronization, cells were treated with 20 μM forskolin (S2449, Selleck) for 2 h.
動物実験	動物モデル	Male Sprague-Dawley rats
	投薬量	10 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/12825829/
https://pubmed.ncbi.nlm.nih.gov/23023353/
https://pubmed.ncbi.nlm.nih.gov/35286396/

https://pubmed.ncbi.nlm.nih.gov/27590029/

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カスタマーフィードバック

: Data from [Eur J Cancer, 2014, 50(7), 1310-20]

: Data from [Data independently produced by , , Clin Cancer Res, 2018, doi:10.1158/1078-0432.CCR-18-2912]

: Data from [Data independently produced by , , J Exp Clin Cancer Res, 2019, 37(1):288]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Innate immune sensing of rotavirus by intestinal epithelial cells leads to diarrhea [ Cell Host Microbe, 2025, 33(3):408-419.e8]	PubMed: 40037352
iPSC-induced multilineage liver organoids, small intestinal organoids and brain organoids sustain pangenotype hepatitis E virus propagation [ Gut, 2025, gutjnl-2025-336105]	PubMed: 41125337
A pancreatic cancer organoid biobank links multi-omics signatures to therapeutic response and clinical evaluation of statin combination therapy [ Cell Stem Cell, 2025, S1934-5909(25)00265-6]	PubMed: 40812300
Sox9 inhibits Activin A to promote biliary maturation and branching morphogenesis [ Nat Commun, 2025, 16(1):1667]	PubMed: 39955269
The cAMP-PKA signaling initiates mitosis by phosphorylating Bora [ Nat Commun, 2025, 16(1):7898]	PubMed: 40849432
Pericytes promote metastasis by regulating tumor local vascular tone and hemodynamics [ Nat Commun, 2025, 16(1):7115]	PubMed: 40753165
Maternal milk fat globule membrane enriched gut L. murinus and circulating SCFAs to improve placental efficiency and fetal development in intrauterine growth restricted mice model [ Gut Microbes, 2025, 17(1):2449095]	PubMed: 39762283
KRIT1 heterozygous mutations are sufficient to induce a pathological phenotype in patient-derived iPSC models of cerebral cavernous malformation [ Cell Rep, 2025, 44(5):115576]	PubMed: 40238631
Lactate shuttle between cytotrophoblast and syncytiotrophoblast in the placenta enhances ferroptosis resistance and maintains placental homeostasis: implications for early pregnancy loss [ Cell Commun Signal, 2025, 23(1):438]	PubMed: 41088442
Aurantio-obtusin modulates Wilms Tumour 1 within the breast tumour microenvironment reducing immunosuppression and tumour growth [ Cell Commun Signal, 2025, 23(1):309]	PubMed: 40598415

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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