Leptomycin B

製品コードS7580 バッチS758001

印刷

化学情報

Chemical Structure Synonyms LMB, CI 940, Elactocin, Mantuamycin, NSC 364372 Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C33H48O6
分子量 540.73 CAS No. 87081-35-4
Solubility (25°C)* 体外 DMSO 100 mg/mL (184.93 mM)
Ethanol 100 mg/mL (184.93 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Leptomycin B (LMB, CI 940, Elactocin, Mantuamycin, NSC 364372) is a potent antifungal antibiotic isolated from Streptomyces and acts as a specific inhibitor of the nuclear export factor CRM1. Leptomycin B rapidly induces cytotoxic effects in cancer cell lines via covalent inhibition of CRM1 with IC50 values of 0.1 nM–10 nM.
in vitro

Leptomycin B (LMB) is a potent anti-fungal antibiotic from Streptomyces sp., also can inhibit the expression of COX-2 mRNA and protein induced by serum withdrawal in MDA-MB-231 cells.[1][2]
in vivo

Leptomycin B (LMB) Reduces Matrix Metalloproteinase-9 Expression and Suppresses Cutaneous Inflammation

プロトコル(参考用のみ)

細胞アッセイ 細胞株 MDA-MB-231 Cells
濃度 2.5, 5, 10, and 20 ng/ml
反応時間 24 h
実験の流れ MDA-MB-231 cells were grown in culture medium containing 10% FBS without this compound (none or vehicle (v)) or in culture medium containing 0% FBS along with this chemical in different concentrations (0, 2.5, 5, 10, and 20 ng/ml) for 24 h. Total RNA was isolated and analyzed for the COX-2 mRNA expression by Northern blot analysis, COX-2 protein expression by Western blot analysis.
動物実験 動物モデル Hairless mice
投薬量 20 mM, 2 mM, 200 nM, or 20 nM
投与方法 Topical

参考

  • https://pubmed.ncbi.nlm.nih.gov/28702009/
  • https://pubmed.ncbi.nlm.nih.gov/12468543/
  • https://pubmed.ncbi.nlm.nih.gov/15675951/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

N4BP1 is a nucleocytoplasmic shuttling protein and recognizes aggregates of the ubiquitin-like protein NEDD8 to protect cells under heat shock [ J Biol Chem, 2025, 301(9):110511] PubMed: 40701250
Nuclear-Cytoplasmic Shuttling of the Usher Syndrome 1G Protein SANS Differs from Its Paralog ANKS4B [ Cells, 2024, 13(22)1855] PubMed: 39594604
Unconventional localization of PAI-1 in PML bodies: A possible link with cellular growth of endothelial cells [ Biochem Biophys Rep, 2024, 39:101793] PubMed: 39161580
Enigmatic missense mutations can cause disease via creation of de novo nuclear export signals [ bioRxiv, 2024, 2024.04.24.590854] PubMed: 38712034
N-acetyltransferase 10 promotes cutaneous wound repair via the NF-κB-IL-6 axis [ Cell Death Discov, 2023, 9(1):324] PubMed: 37644005
N-acetyltransferase 10 promotes cutaneous wound repair via the NF-κB-IL-6 axis [ Cell Death Discov, 2023, 9(1):324] PubMed: 37644005
Dickkopf1 fuels inflammatory cytokine responses [ Commun Biol, 2022, 5(1):1391] PubMed: 36539532

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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