Palmitic acid

製品コードS3794 バッチS379402

印刷

化学情報

Synonyms

hexadecanoic acid, Cetylic acid

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₆H₃₂O₂

分子量

256.42

CAS No.

57-10-3

Solubility (25°C)*

体外

DMSO

51 mg/mL (198.89 mM)

Ethanol

51 mg/mL (198.89 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	2.550mg/ml (9.94mM)	Taking the 1 mL working solution as an example, add 50 μL of 51 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	1.270mg/ml (4.95mM)	Taking the 1 mL working solution as an example, add 50 μL of 25.4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Palmitic acid (Hexadecanoic acid, Cetylic acid) is the most common saturated fatty acid found in animals, plants and microorganisms with anti-tumor activity. This product is a fatty acid that is almost insoluble in water-soluble solvents such as culture medium. It needs to be emulsified before use in cell experiments. It is recommended to purchase it in powder form.
in vitro	Palmitic acid (PA) binds TLR4 via the adaptor protein MD2 and that the TLR4 signal induced by this compound stimulates dendritic cells activation and maturation accompanied by a robust IL-1β response. It is a TLR4/MD-2 ligand. This chemical exerts pro-inflammatory activity through TLR4^[1].
in vivo	In a dose-dependent fashion, palmitic acid rapidly reduces mouse locomotor activity by a mechanism that did not rely on TLR4, MyD88, IL-1, IL-6 or TNFα but is dependent on fatty acid chain length. This compound induces anxiety-like behavior in mice while increasing amygdala-based serotonin metabolism^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	The insulinoma cell line INS-1
	濃度	250 μmol/L
	反応時間	24 or 48 h
	実験の流れ	The INS-1 cells are seeded onto 96-well plates at 4,000 cells/well with 10% BSA and incubated with T0901317 (10 μmol/L), palmitic acid (250 μmol/L), a combination of T0901317 and this compound or PBS (mock control) for 24 or 48 h. After the treatment incubation, 20 μl of MTT solution (5 mg/mL MTT in PBS, pH 7.4) are added to each well, followed by incubation at 37°C for 4 h. The supernatants are removed from the wells and DMSO (15 μl/well) is added to dissolve the crystals. The optical density at a wavelength of 490 nm is measured using a microplate reader.
動物実験	動物モデル	C57BL/6J mice
	投薬量	0.3, 3, and 30 μmol/mouse
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/28463985/
https://pubmed.ncbi.nlm.nih.gov/25016520/
https://pubmed.ncbi.nlm.nih.gov/21753123/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Stromal Cell Derived Factor-1 Promotes Hepatic Insulin Resistance via Inhibiting Hepatocyte Lipophagy [ J Cell Mol Med, 2025, 29(2):e70352]	PubMed: 39855896
Ginkgolide B attenuates hyperlipidemia by restoring sphingolipid homeostasis and activating PPARα and Nrf2 pathways [ Sci Rep, 2025, 15(1):28774]	PubMed: 40770408
Quercetin alleviates metabolic-associated fatty liver disease by tuning hepatic lipid metabolism, oxidative stress and inflammation [ Anim Biotechnol, 2025, 36(1):2442351]	PubMed: 39718035
Prenatal valproic acid on the basis of gestational diabetes also induces autistic behavior and disrupts myelination and oligodendroglial maturation slightly in offspring [ Research Square, 2024, 10.21203/rs.3.rs-3480364/v1]	PubMed: none
Myristic acid as a checkpoint to regulate STING-dependent autophagy and interferon responses by promoting N-myristoylation [ Nat Commun, 2023, 14(1):660]	PubMed: 36750575
Palmitic acid induces nDNA release to cytosol and promotes microglial M1 polarization via cGAS-STING signaling pathway [ Biochim Biophys Acta Mol Cell Res, 2023, 1870(1):119385]	PubMed: 36302463
FGF9 Alleviates the Fatty Liver Phenotype by Regulating Hepatic Lipid Metabolism [ Front Pharmacol, 2022, 13:850128]	PubMed: 35517790
GDF11 rapidly increases lipid accumulation in liver cancer cells through ALK5-dependent signaling [ Biochim Biophys Acta Mol Cell Biol Lipids, 2021, 1866(6):158920]	PubMed: 33684566
Effects of melatonin on fatty liver disease: The role of NR4A1/DNA-PKcs/p53 pathway, mitochondrial fission, and mitophagy [ J Pineal Res, 2018, 64(1)]	PubMed: 28981157

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。