Nirmatrelvir (PF-07321332)

製品コードS9866 バッチS986601

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C23H32F3N5O4
分子量 499.53 CAS No. 2628280-40-8
Solubility (25°C)* 体外 DMSO 100 mg/mL (200.18 mM)
Ethanol 100 mg/mL (200.18 mM)
Water 2 mg/mL (4.0 mM)
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明

Nirmatrelvir (PF-07321332) is an reversible covalent inhibitor of SARS-CoV-2 main protease (Mpro, also referred to as 3CL protease) with an ki of 3.11 nM. PF-07321332 binds directly to the catalytic cysteine (Cys145) residue of the enzyme.
in vitro
Nirmatrelvir (PF-07321332) is a reversible covalent inhibitor of SARS-CoV-2 main protease (Mpro, also known as 3CL protease), and ki is 3.11 nM. 
This compound directly binds to the catalytic cysteine (Cys145) residue of the enzyme.[1]

プロトコル(参考用のみ)

キナーゼアッセイ Enzyme kinetics assay
Nirmatrelvir (PF-07321332) and SARS-CoV-2 Mpro enzyme were incubated at a 1:1 molar ratio of 2 μM compound and 2 μM enzyme in assay buffer for 20 minutes. The mixture was then diluted 50-fold into assay buffer followed by a transfer of 5 μl to wells of a black low volume 384-well assay plate. Enzyme activity was monitored on a BMG Pherastar at Ex/Em of 340 nm/460 nm after addition of 5 μl 60 μM peptide substrate. Final reaction conditions were 20 nM enzyme with 20 nM this compound and 30 μM peptide substrate.
細胞アッセイ 細胞株 HCoV-229E in Huh-7 cells
濃度 0.62 µM
反応時間 2 days
実験の流れ

Nirmatrelvir (PF-07321332) experiments were performed on Huh-7 cells at an MOI of 0.002 and infection determined by in-cell ELISA 2 days later
動物実験 動物モデル Female C57BL/6 Cg-Tg2Prlmn/J mice 
投薬量 20 mg/kg
投与方法 Oral

参考

  • https://pubmed.ncbi.nlm.nih.gov/34726479/
  • https://pubmed.ncbi.nlm.nih.gov/35598779/
  • https://pubmed.ncbi.nlm.nih.gov/36209984/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Selective Inhibition of Vascular Smooth Muscle Cell Function by COVID-19 Antiviral Drugs: Impact of Heme Oxygenase-1 [ Antioxidants (Basel), 2025, 14(8)945] PubMed: 40867841
Characterization of an unusual SARS-CoV-2 main protease natural variant exhibiting resistance to nirmatrelvir and ensitrelvir [ Commun Biol, 2025, 8(1):1061] PubMed: 40676153
An ACE2-Fc decoy produced in glycoengineered plants neutralizes ancestral and newly emerging SARS-CoV-2 variants and demonstrates therapeutic efficacy in hamsters [ Sci Rep, 2025, 15(1):11307] PubMed: 40175560
Design, Synthesis, and Anti-SARS-CoV-2 Activity of Amodiaquine Analogs [ Chem Pharm Bull (Tokyo), 2025, 73(4):355-368] PubMed: 40240159
The host-targeted antiviral drug Zapnometinib exhibits a high barrier to the development of SARS-CoV-2 resistance [ Antiviral Res, 2024, 225:105840] PubMed: 38438015
Characterization of alternate encounter assemblies of SARS-CoV-2 main protease [ J Biol Chem, 2024, S0021-9258(24)02176-8] PubMed: 39128719
Novel Pan-Coronavirus 3CL Protease Inhibitor MK-7845: Biological and Pharmacological Profiling [ Viruses, 2024, 16(7)1158] PubMed: 39066320
SARS-CoV-2 Mpro inhibitor identification using a cellular gain-of-signal assay for high-throughput screening [ SLAS Discov, 2024, S2472-5552(24)00043-1] PubMed: 39173830
Stabilization of the Dimeric State of SARS-CoV-2 Main Protease by GC376 and Nirmatrelvir [ Int J Mol Sci, 2023, 24(7)6062] PubMed: 37047038
Immunodetection assays for the quantification of seasonal common cold coronaviruses OC43, NL63, or 229E infection confirm nirmatrelvir as broad coronavirus inhibitor [ Antiviral Res, 2022, 203:105343] PubMed: 35598779

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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