(-)-Menthol

製品コードS4714 バッチS471403

印刷

化学情報

Chemical Structure Synonyms L-Menthol, Levomenthol, Menthomenthol, Menthacamphor Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C10H20O
分子量 156.27 CAS No. 2216-51-5
Solubility (25°C)* 体外 DMSO 31 mg/mL (198.37 mM)
Ethanol 31 mg/mL (198.37 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 (-)-Menthol (L-Menthol, Levomenthol, Menthomenthol, Menthacamphor) is a levo isomer of menthol and used as a cooling agent that strongly activates TRPM8.
in vitro (-)-Menthol induces cytotoxicity against murine leukemia WEHI-3 cells in vitro in a dose-dependent manner. This compound inhibits the growth of rat liver epithelial tumor cells and acts as a potent chemopreventive agent during DMBA initiation of rat mammary tumors. It also is found to inhibit the N-acetyltransferase activity. It also is found to inhibit the DNA topoisomerase I, II alpha and beta and to promote NF-kappaB expression in human gastric cancer SNU-5 cells. Additionally, this chemical induces human promyelocytic leukemia HL-60 cell death through the Ca2+ release from the endoplasmic reticulum[1].
in vivo The effects of (-)-menthol on WEHI-3 cells in vivo (BALB/c mice) were also examined, and it was observed that the Mac-3 and CD11b markers were decreased, indicating inhibition of differentiation of the precursor of macrophage and granulocyte. The weights of liver and spleen samples from mice treated with this compound were found to be decreased compared to untreated animals. This chemical has been shown to be toxic in animals, but in humans, it is considered to be safe, with a small cardio-accelerating effect. At high doses it may exert a depressant effect on the central nervous system in rodents. This compound statistically decreases the weights of the liver and spleen in the examined animals and also decreases the percentage of MAC-3 and CD11b cells in the blood.[1].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Murine leukemia cell line (WEHI-3)
濃度 0, 25, 50, 75 and 100 μM
反応時間 24 h
実験の流れ

Approximately 2x105 cells (WEHI-3) are incubated in 12-well plates containing medium for 24 h without (control) or with various concentrations (0, 25, 50, 75 and 100 μM) of (-)-menthol. The cells are harvested and washed with PBS before PI staining. The percentage of viable WEHI-3 cells is determined by trypan blue exclusion and flow cytometry.
動物実験 動物モデル Male BALB/c mice
投薬量 1 mg/kg or 10 mg/kg
投与方法 Oral administration

参考

  • https://pubmed.ncbi.nlm.nih.gov/17436578/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Growth at Cold Temperature Increases the Number of Motor Neurons to Optimize Locomotor Function [ Curr Biol, 2019, 29(11):1787-1799.e5] PubMed: 31130453

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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