1-Deoxynojirimycin

製品コードS3839 バッチS383902

化学情報

	Synonyms	duvoglustat, moranolin	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₆H₁₃NO₄
	分子量	163.17	CAS No.	19130-96-2
Solubility (25°C)*	体外	DMSO	33 mg/mL (202.24 mM)
		Water	33 mg/mL (202.24 mM)
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	1-Deoxynojirimycin (duvoglustat, moranolin) is a potent α-glucosidase inhibitor and most commonly found in mulberry leaves. It has therapeutic potency against diabetes mellitus.
in vitro	1-Deoxynojirimycin (DNJ) up to 200 μmol/L does not influence survival of HUVECs. DNJ behaves as an antioxidant, decreases ROS production, and delays cellular senescence^[2].
in vivo	1-Deoxynojirimycin (DNJ) inhibits intestinal glucose absorption in intestine. 1-Deoxynojirimycin down-regulates intestinal SGLT1, Na+/K+-ATP and GLUT2 mRNA and protein expression. Pretreatment with DNJ (50 mg/kg) increases the activity, mRNA and protein levels of hepatic glycolysis enzymes (GK, PFK, PK, PDE1) and decreases the expression of gluconeogenesis enzymes (PEPCK, G-6-Pase), improves glucose tolerance in both normal and diabetic mice. DNJ inhibits intestinal glucose absorption and accelerates hepatic glucose metabolism by directly regulating the expression of proteins involved in glucose transport systems, glycolysis and gluconeogenesis enzymes^[1]. DNJ reduces oxidative stress in the liver and plasma in rodents^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HUVECs
	濃度	0-200 μmol/L
	反応時間	24 or 48 h
	実験の流れ	Cytotoxicity of DNJ is assessed using a WST-8 assay. HUVECs at 80% confluency are trypsinized and transferred to 96-well plates (4000 cells/well). Stock solutions of DNJ are prepared in HuMedia-EG2 medium. DNJ test media are prepared from the stock solutions and diluted to final concentrations of 0-200 μmol/L in HuMedia-EG2 medium. After incubation for 24 h at 37℃, the cells are placed in 200 μL of fresh HuMedia-EG2 medium with various concentrations of DNJ. After 24 or 48 h, 10 μL WST-8 solution is added to each well. After incubation for 3 h at 37℃, cytotoxicity is measured using a microplate reader at a wavelength of 450 nm and a reference wavelength of 655 nm.
動物実験	動物モデル	ICR mice
	投薬量	50 mg/kg
	投与方法	administered intragastrically

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Transmembrane serine protease TMPRSS2 implicated in SARS-CoV-2 infection is autoactivated intracellularly and requires N-glycosylation for regulation [ J Biol Chem, 2022, 298(12):102643]	PubMed: 36309092

Transmembrane serine protease TMPRSS2 implicated in SARS-CoV-2 infection is autoactivated intracellularly and requires N-glycosylation for regulation [ J Biol Chem, 2022, 298(12):102643]

PubMed: 36309092

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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