1400W 2HCl

製品コードS8337 バッチS833701

印刷

化学情報

Chemical Structure Synonyms N-(3-(Aminomethyl)benzyl)acetamidine Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C10H15N3.2HCl
分子量 250.17 CAS No. 214358-33-5
Solubility (25°C)* 体外 DMSO 35 mg/mL (139.9 mM)
Water 35 mg/mL (139.9 mM)
Ethanol 35 mg/mL (139.9 mM)
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 1400W (N-(3-(Aminomethyl)benzyl)acetamidine) is a slow, tight binding, and highly selective inhibitor of inducible nitric-oxide synthase(iNOS).
in vitro 1400W is either an irreversible inhibitor or an extremely slowly reversible inhibitor of human iNOS. Inhibition of human iNOS by 1400W is time-dependent. 1400W is competitive with L-arginine. 1400W is not a substrate for iNOS[1].
in vivo 1400W selectively prevents microvasculature injury in rats. 1400W is greater than 50-fold more potent against iNOS than eNOS in a rat model of endotoxin-induced vascular injury. Moreover, 1400W also dose-dependently reduces LPS-induced vascular leakage associated with iNOS induction in the colon, lung, liver, kidney, and heart. The maximal protection is close to 100% for all organs except the kidney (kidney:54%)[1]. 1400W has an ameliorative effect on both oxidative and nitrosative stress in the kidneys against renal I/R injury in rats[2]. Treatment with 1400W can reduce the rate of growth of solid tumors in mice[4].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 RAW264.7 cells
濃度 100 μM
反応時間 16 h
実験の流れ RAW264.7 cells are treated with LPS/IFNγ for 16 h and GAPDH-p300 binding is abolished by the iNOS inhibitor 1400W (100 μM). Cell lysates are immunoprecipitated with an anti-p300 antibody and the immunoprecipitates are analysed by western blotting with an anti-GAPDH antibody.
動物実験 動物モデル Rats
投薬量 0.1-10 mg/kg
投与方法 s.c.

参考

  • https://pubmed.ncbi.nlm.nih.gov/9030556/
  • https://pubmed.ncbi.nlm.nih.gov/19814638/
  • https://pubmed.ncbi.nlm.nih.gov/18552833/
  • https://pubmed.ncbi.nlm.nih.gov/9242464/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

GRA12 is a common virulence factor across Toxoplasma gondii strains and mouse subspecies [ Nat Commun, 2025, 16(1):3570] PubMed: 40240328
iNOS-Produced Nitric Oxide from Cancer Cells as an Intermediate of Stemness Regulation by PARP-1 in Colorectal Cancer [ Biomolecules, 2025, 15(1)125] PubMed: 39858519
iNOS is necessary for GBP-mediated T. gondii clearance in murine macrophages via vacuole nitration and intravacuolar network collapse [ Nat Commun, 2024, 15(1):2698] PubMed: 38538595
Uncovering therapeutic targets for macrophage-mediated T cell suppression and PD-L1 therapy sensitization [ Cell Rep Med, 2024, S2666-3791(24)00419-1] PubMed: 39181134
Normalization of CF Immune System Reverses Intestinal Neutrophilic Inflammation and Significantly Improves the Survival of CF Mice [ Cell Mol Gastroenterol Hepatol, 2024, S2352-345X(24)00179-6] PubMed: 39510500
Nitric oxide facilitates the S-nitrosylation and deubiquitination of Notch1 protein to maintain cancer stem cells in human NSCLC [ J Cell Mol Med, 2024, 28(21):e70203] PubMed: 39523215
Ninjinyoeito Prevents Onset of Depression-Like Behavior and Reduces Hippocampal iNOS Expression in Senescence-Accelerated Mouse Prone 8 Mice [ Evid Based Complement Alternat Med, 2023, 2023:2151004] PubMed: 37593014
Ninjinyoeito Prevents Onset of Depression-Like Behavior and Reduces Hippocampal iNOS Expression in Senescence-Accelerated Mouse Prone 8 Mice [ Evid Based Complement Alternat Med, 2023, 2023:2151004] PubMed: 37593014
Inducible nitric oxide synthase (iNOS) is necessary for GBP-mediated T. gondii restriction in murine macrophages via vacuole nitration and intravacuolar network collapse [ bioRxiv, 2023, 2023.07.24.549965] PubMed: 37546987
Inducible nitric oxide synthase (iNOS) is necessary for GBP-mediated T. gondii restriction in murine macrophages via vacuole nitration and intravacuolar network collapse [ bioRxiv, 2023, 2023.07.24.549965] PubMed: 37546987

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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