1400W 2HCl

製品コードS8337 バッチS833702

印刷

化学情報

Chemical Structure Synonyms N-(3-(Aminomethyl)benzyl)acetamidine Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C10H15N3.2HCl
分子量 250.17 CAS No. 214358-33-5
Solubility (25°C)* 体外 DMSO 50 mg/mL (199.86 mM)
Water 50 mg/mL (199.86 mM)
Ethanol 13 mg/mL (51.96 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 1400W (N-(3-(Aminomethyl)benzyl)acetamidine) is a slow, tight binding, and highly selective inhibitor of inducible nitric-oxide synthase(iNOS).
in vitro 1400W is either an irreversible inhibitor or an extremely slowly reversible inhibitor of human iNOS. Inhibition of human iNOS by 1400W is time-dependent. 1400W is competitive with L-arginine. 1400W is not a substrate for iNOS[1].
in vivo 1400W selectively prevents microvasculature injury in rats. 1400W is greater than 50-fold more potent against iNOS than eNOS in a rat model of endotoxin-induced vascular injury. Moreover, 1400W also dose-dependently reduces LPS-induced vascular leakage associated with iNOS induction in the colon, lung, liver, kidney, and heart. The maximal protection is close to 100% for all organs except the kidney (kidney:54%)[1]. 1400W has an ameliorative effect on both oxidative and nitrosative stress in the kidneys against renal I/R injury in rats[2]. Treatment with 1400W can reduce the rate of growth of solid tumors in mice[4].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 RAW264.7 cells
濃度 100 μM
反応時間 16 h
実験の流れ RAW264.7 cells are treated with LPS/IFNγ for 16 h and GAPDH-p300 binding is abolished by the iNOS inhibitor 1400W (100 μM). Cell lysates are immunoprecipitated with an anti-p300 antibody and the immunoprecipitates are analysed by western blotting with an anti-GAPDH antibody.
動物実験 動物モデル Rats
投薬量 0.1-10 mg/kg
投与方法 s.c.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Ninjinyoeito Prevents Onset of Depression-Like Behavior and Reduces Hippocampal iNOS Expression in Senescence-Accelerated Mouse Prone 8 Mice [ Evid Based Complement Alternat Med, 2023, 2023:2151004] PubMed: 37593014
Ninjinyoeito Prevents Onset of Depression-Like Behavior and Reduces Hippocampal iNOS Expression in Senescence-Accelerated Mouse Prone 8 Mice [ Evid Based Complement Alternat Med, 2023, 2023:2151004] PubMed: 37593014
Inducible nitric oxide synthase (iNOS) is necessary for GBP-mediated T. gondii restriction in murine macrophages via vacuole nitration and intravacuolar network collapse [ bioRxiv, 2023, 2023.07.24.549965] PubMed: 37546987
Inducible nitric oxide synthase (iNOS) is necessary for GBP-mediated T. gondii restriction in murine macrophages via vacuole nitration and intravacuolar network collapse [ bioRxiv, 2023, 2023.07.24.549965] PubMed: 37546987
Inflammatory fibroblasts mediate resistance to neoadjuvant therapy in rectal cancer [ Cancer Cell, 2022, 40(2):168-184.e13] PubMed: 35120600
Identification of potential anti-pneumonia pharmacological components of Glycyrrhizae Radix et Rhizoma after the treatment with Gan An He Ji oral liquid [ J Pharm Anal, 2022, 12(6):839-851] PubMed: 36605579
Posttranslational S-nitrosylation modification regulates HMGB1 secretion and promotes its proinflammatory and neurodegenerative effects [ Cell Rep, 2022, 40-11:111330] PubMed: 36103834
A Nurr1 ligand C-DIM12 attenuates brain inflammation and improves functional recovery after intracerebral hemorrhage in mice [ Sci Rep, 2022, 12(1):11009] PubMed: 35773404
Peroxynitrite activates NLRP3 inflammasome and contributes to hemorrhagic transformation and poor outcome in ischemic stroke with hyperglycemia [ Free Radic Biol Med, 2021, 165:171-183] PubMed: 33515754
Novel Isoquinoline Alkaloid Litcubanine A - A Potential Anti-Inflammatory Candidate [ Front Immunol, 2021, 12:685556] PubMed: 34163484

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。