2-APB (2-Aminoethyl Diphenylborinate)

製品コードS6657 バッチS665702

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C14H16BNO
分子量 225.09 CAS No. 524-95-8
Solubility (25°C)* 体外 DMSO 45 mg/mL (199.92 mM)
Ethanol 45 mg/mL (199.92 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 2-APB (2-Aminoethyl Diphenylborinate) is an IP3 receptor inhibitor and regulate IP3-induced calcium release.2-APB also inhibits the SOC channel activity and activates TRP channel at higher concentrations.
in vitro

2-APB, an inositol 1, 4, 5-trisphosphate receptor (IP3R) inhibitor, obviously attenuates the intracellular Ca2+ release and cardiomyocyte apoptosis induced by oxygen and glucose deprivation (OGD) stimulation and EndoA2 knockdown.[3]
in vivo

2-APB increases the basilar artery (BA) wall thickness and reduced the BA lumen diameter, inducing significant vascular changes, also alleviates cell apoptosis at 24 hours after SAH.[4]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 neonatal rat cardiomyocytes
濃度 2 h
反応時間 --
実験の流れ

Neonatal Sprague-Dawley rats (1 to 3 days old) are exposed to hypothermia until they became unresponsive and are then euthanized by cervical dislocation. Primary culture of NRCMs is performed. The cells are seeded at a density of 5 x 106 cells/ml in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum and 0.1 mM 5-bromodeoxyuridine. After 24 h, NRCMs are subjected to various treatments as described below. To assess the effects of EndoA2 on cardiac injury, the cells are transfected with EndoA2 siRNA or Ad-EndoA2. After transfection for 36 h, the cells are stimulated with oxygen and glucose deprivation (OGD) for another 12 h. Incubation of sodium phenylbutyrate (4-PBA), Cyclosporin A (CsA), 1,2-bis(o-aminophenoxy)ethane-N,N,N ,N -tetraacetic acid (BAPTA) or 2-Aminoethoxydiphenylborate (2-APB) is 2 h before OGD stimulation.Cardiomyocytes are cultured in serum-free, glucose and sodium pyruvate-free DMEM at 37 ℃ in an anoxia chamber saturated with 94% N2/5% CO2/ 1% O2.
動物実験 動物モデル Rats of subarachnoid hemorrhage (SAH) model
投薬量 0.5, 2 mg/kg
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Porcine reproductive and respiratory syndrome virus infection triggers autophagy via ER stress-induced calcium signaling to facilitate virus replication [ PLoS Pathog, 2023, 19(3):e1011295] PubMed: 36972295
Only one carbon difference determines the pro-apoptotic activity of α-tocopheryl esters [ FEBS J, 2022, 10.1111/febs.16623] PubMed: 36083714
Endophilin A2-mediated alleviation of endoplasmic reticulum stress-induced cardiac injury involves the suppression of ERO1α/IP3R signaling pathway [ Int J Biol Sci, 2021, 17(13):3672-3688] PubMed: 34512174
Endophilin A2-mediated alleviation of endoplasmic reticulum stress-induced cardiac injury involves the suppression of ERO1α/IP3R signaling pathway [ Int J Biol Sci, 2021, 17(13):3672-3688] PubMed: 34512174
Evodiamine induces ROS-Dependent cytotoxicity in human gastric cancer cells via TRPV1/Ca2+ pathway [ Chem Biol Interact, 2021, 351:109756] PubMed: 34808100
STIM1 Regulates Endothelial Calcium Overload and Cytokine Upregulation During Sepsis [ J Surg Res, 2021, 263:236-244] PubMed: 33713955
Calcium channel blocker amlodipine besylate therapy is associated with reduced case fatality rate of COVID-19 patients with hypertension [ Cell Discov, 2020, 6(1):96] PubMed: 33349633
CaSR participates in the regulation of vascular tension in the mesentery of hypertensive rats via the PLC‑IP3/AC‑V/cAMP/RAS pathway [ Mol Med Rep, 2019, 20(5):4433-4448] PubMed: 31485595

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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