2-D08

製品コードS8696 バッチS869601

印刷

化学情報

Chemical Structure Synonyms 2',3',4'-trihydroxy flavone Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C15H10O5
分子量 270.24 CAS No. 144707-18-6
Solubility (25°C)* 体外 DMSO 54 mg/mL (199.82 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5% DMSO 95% Corn oil
0.33mg/ml Taking the 1 mL working solution as an example, add 50 μL of 6.6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
2.85mg/ml Taking the 1 mL working solution as an example, add 50 μL of 57 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 2-D08 (2',3',4'-trihydroxy flavone) is a cell permeable, mechanistically unique inhibitor of protein sumoylation.
in vitro

2-D08, a inhibitor of protein SUMOylation, enhances the all-trans retinoic acid (ATRA)-induced expression of key genes involved in differentiation, proliferation, and apoptosis in non-APL AML cells[1]. 2-D08 inhibits sumoylation by preventing transfer of SUMO from the UBC9-SUMO thioester to the substrate[2]. 

プロトコル(参考用のみ)

細胞アッセイ 細胞株 U937, HL60, THP1 cell lines
濃度 50 µM
反応時間 5days, 9 days
実験の流れ

Cells are treated for 5 days 2-D08 (50 µM). CD14, CD11b or CD15 expressions are assayed by western bolt or flow cytometry. Morphological changes of cells are shown by May-Grumwald-Giemsa (MGG)-stained. 
動物実験 動物モデル α2−/− mice (Eight- to 10-week-old mice)
投薬量 30 μM (10 μl)
投与方法 Injected on one hemisphere near the hippocampal area

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis [ Acta Pharmaceutica Sinica B, 2024, 10.1016/j.apsb.2024.01.006] PubMed: none
SUMOylation of the ubiquitin ligase component KEAP1 at K39 upregulates NRF2 and its target function in lung cancer cell proliferation [ J Biol Chem, 2023, 299(10):105215] PubMed: 37660919
SUMOylation of the ubiquitin ligase component KEAP1 at K39 upregulates NRF2 and its target function in lung cancer cell proliferation [ J Biol Chem, 2023, 299(10):105215] PubMed: 37660919
hnRNP G/RBMX enhances HPV16 E2 mRNA splicing through a novel splicing enhancer and inhibits production of spliced E7 oncogene mRNAs [ Nucleic Acids Res, 2022, 50(7):3867-3891] PubMed: 35357488
Recapitulating early human development with 8C-like cells [ Cell Rep, 2022, 39(12):110994] PubMed: 35732112
SUMOylation of Kir7.1 participates in neuropathic pain through regulating its membrane expression in spinal cord neurons [ CNS Neurosci Ther, 2022, 10.1111/cns.13871] PubMed: 35633059
The African swine fever virus protease pS273R inhibits DNA sensing cGAS-STING pathway by targeting IKKε [ Virulence, 2022, 13(1):740-756] PubMed: 35437104
TRIM28 SUMOylates and stabilizes NLRP3 to facilitate inflammasome activation [ Nat Commun, 2021, 12(1):4794] PubMed: 34373456
Silent information regulator 2 promotes clear cell renal cell carcinoma progression through deacetylation and small ubiquitin-related modifier 1 modification of glucose 6-phosphate dehydrogenase [ Cancer Sci, 2021, 112(10):4075-4086] PubMed: 34310804
RANBP2 Activates O-GlcNAcylation through Inducing CEBPα-Dependent OGA Downregulation to Promote Hepatocellular Carcinoma Malignant Phenotypes [ Cancers (Basel), 2021, 13(14)3475] PubMed: 34298689

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。