2-Methoxy-1,4-naphthoquinone

製品コードS3661 バッチS366101

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₁H₈O₃

分子量

188.18

CAS No.

2348-82-5

Solubility (25°C)*

体外

DMSO

37 mg/mL (196.62 mM)

Ethanol

3 mg/mL (15.94 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	インパチェンス・グランデュリフェラ（Impatiens glandulifera）の葉から単離された2-Methoxy-1,4-naphthoquinoneは、Raji細胞において、濃度依存的にPKC βI、δ、ζの発現を特異的に抑制しました。
in vitro	2-Methoxy-1,4-naphthoquinone (MNQ) exhibits antifungal, antimicrobial activities and showed strong killing effect on K562, HL-60, Raji, MCF-7, H-23, IMR-32, LA-174-T, HSC-2, and SK-MEL-28. MNQ is able to trigger the intrinsic apoptotic pathway in K562 cells, as well as causing p53-dependent cell cycle arrest in the p53-wild type cell line. MNQ inhibits the TCF/β-catenin (TOP) transcriptional activity at IC50 2.9 μM, while it decreases the transcriptional activity of FOP (mutated TCF-binding site)-transfected cell. MNQ exerts an anti-metastatic effect against MDA-MB-231 cells. MNQ has remarkable selectivity towards PKCβI and ζ suppression. MNQ suppresses PKCδ moderately, where MNQ suppresses half of the PKCδ expression at the concentration of 10 μM. MNQ does not suppress PKC βII. MNQ is able to inhibit the invasion and migration characteristics of a highly metastatic MDA-MB-231 cancer cell line.

製品説明

インパチェンス・グランデュリフェラ（Impatiens glandulifera）の葉から単離された2-Methoxy-1,4-naphthoquinoneは、Raji細胞において、濃度依存的にPKC βI、δ、ζの発現を特異的に抑制しました。

in vitro

2-Methoxy-1,4-naphthoquinone (MNQ) exhibits antifungal, antimicrobial activities and showed strong killing effect on K562, HL-60, Raji, MCF-7, H-23, IMR-32, LA-174-T, HSC-2, and SK-MEL-28. MNQ is able to trigger the intrinsic apoptotic pathway in K562 cells, as well as causing p53-dependent cell cycle arrest in the p53-wild type cell line. MNQ inhibits the TCF/β-catenin (TOP) transcriptional activity at IC50 2.9 μM, while it decreases the transcriptional activity of FOP (mutated TCF-binding site)-transfected cell. MNQ exerts an anti-metastatic effect against MDA-MB-231 cells. MNQ has remarkable selectivity towards PKCβI and ζ suppression. MNQ suppresses PKCδ moderately, where MNQ suppresses half of the PKCδ expression at the concentration of 10 μM. MNQ does not suppress PKC βII. MNQ is able to inhibit the invasion and migration characteristics of a highly metastatic MDA-MB-231 cancer cell line.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Raji cells
	濃度	--
	反応時間	48 h
	実験の流れ	5×10⁵ Raji cells are incubated in 1 mL of RPMI 1640 medium (supplemented with 10% FBS) containing 0.05 μM PMA, 3 mM SnB and test substance (MNQ or DMSO as the vehicle control) at 37 °C under 5% CO2 for 48 hours. Genistein is used as the standard drug. The cells are then harvested, washed with PBS and fixed onto Teflon-coated slides.

参考

http://imsear.li.mahidol.ac.th/handle/123456789/166269
https://pubmed.ncbi.nlm.nih.gov/24291160/

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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