2-NBDG

製品コードS8914 バッチS891401

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₁₂H₁₄N₄O₈

分子量

342.26

CAS No.

186689-07-6

Solubility (25°C)*

体外

DMSO

68 mg/mL (198.67 mM)

Ethanol

3 mg/mL (8.76 mM)

Water

2 mg/mL (5.84 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	3.400mg/ml (9.93mM)	Taking the 1 mL working solution as an example, add 50 μL of 68 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.850mg/ml (2.48mM)	Taking the 1 mL working solution as an example, add 50 μL of 17 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	蛍光デオキシグルコース誘導体である2-NBDGは、グルコース取り込みを検出するためのマーカーであり、細胞生存率の指標です。
in vitro	1.1 Preparation of the stock solution Dissolve 1 mg of 2-NBDG in 2.92 mL of DDH2O to obtain 1 mM of this compound. Note: It is recommended to store the stock solution at -20 ℃ or -80 ℃ away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of 2-NBDG working solution. Dilute the stock solution in serum-free cell culture medium or PBS to obtain 10-200 μM of this chemical working solution. Note: Please adjust the concentration of this reagent working solution according to the actual situation. Cell staining 2.1 For suspension cells: Centrifuge at 1,000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. 2.2 Add 1 mL of this compound working solution, and then incubate at room temperature for 5-60 minutes. 2.3 Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant. 2.4 Wash twice with PBS, 5 minutes each time. 2.5 Resuspend cells with serum-free cell culture medium or PBS. .If test viability, recorded the optical density (O.D.) at 540/570 nm. Cell viability was calculated as a control ratio and plotted against the logarithmic concentration of the drug to calculate IC50.
in vivo	2-NBDG fluorescence intensity following 30-minutes topical application was 6-fold and 4-fold higher in OSCC and OED, respectively, compared to normal mucosa. Receiver operator characteristic analysis show 83% sensitivity and 73% specificity for detection of neoplasia vs benign (normal and inflammation). Faster fluorescence temporal decay in neoplasia indicated higher uptake and glucose metabolic rate than normal mucosa. Mucosal delivery of this compound by topical application to the in-vivo oral surface is feasible and delineates neoplasia from normal mucosa, providing in-vivo noninvasive molecular imaging of dysregulated glucose metabolism.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Primary cortical astrocytes
	濃度	25 μM to 200 μM
	反応時間	1 h
	実験の流れ	Nine culture plates of astrocytes are used for each uptake experiment. After medium is removed and plates are rinsed in wash buffer, cells are incubated at 37 °C in buffer containing 25–200 μM 2-NBDG. After 1 h, buffers are replaced by fresh incubation buffer with 2 mM glucose and incubation is continued for 5 min. The cells are washed twice with prechilled phosphate-buffered saline and are incubated at room temperature in 0.1 mL cell lysis buffer for 10 min.
動物実験	動物モデル	4-week old male Golden Syrian Hamsters
	投薬量	1 mg/ml (1 ml)
	投与方法	Oral gavage

参考

https://pubmed.ncbi.nlm.nih.gov/25091860/
https://pubmed.ncbi.nlm.nih.gov/29950704/
https://pubmed.ncbi.nlm.nih.gov/16182371/

https://pubmed.ncbi.nlm.nih.gov/17406637/

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The essential roles of m6A RNA modification to stimulate ENO1-dependent glycolysis and tumorigenesis in lung adenocarcinoma [ J Exp Clin Cancer Res, 2022, 41(1):36]	PubMed: 35078505
A sensitive and simple HPLC-FLD-based method for the measurement of intracellular glucose uptake [ Food Chem, 2021, 372:131218]	PubMed: 34624783
A Novel Mechanism of Cannabidiol in Suppressing Hepatocellular Carcinoma by Inducing GSDME Dependent Pyroptosis [ Front Cell Dev Biol, 2021, 9:697832]	PubMed: 34350183
Integrating metabolomic data with machine learning approach for discovery of Q-markers from Jinqi Jiangtang preparation against type 2 diabetes [ Chin Med, 2021, 16(1):30]	PubMed: 33741031

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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