3',3'-cGAMP

製品コードS7905 バッチS790501

印刷

化学情報

Synonyms

3',3'-cyclic GMP-AMP, Cyclic GMP-AMP, cGAMP

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₂₀H₂₄N₁₀O₁₃P₂·xNa+

分子量

672.4

CAS No.

849214-04-6(freeacid)

Solubility (25°C)*

体外

Water

100 mg/mL (148.72 mM)

DMSO

20 mg/mL (29.74 mM)

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.160mg/ml (0.24mM)	Taking the 1 mL working solution as an example, add 50 μL of 3.2 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.160mg/ml (0.24mM)	Taking the 1 mL working solution as an example, add 50 μL of 3.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	3',3'-cGAMP (3',3'-cyclic GMP-AMP, Cyclic GMP-AMP, cGAMP) activates the endoplasmic reticulum (ER)-resident receptor stimulator of interferon genes (STING), thereby inducing an antiviral state and the secretion of type I IFNs.
in vivo	Sublingual immunization of mice with Bacillus anthracis protective antigen (PA) and the STING ligand 3′3′-cGAMP promotes PA-specific serum IgG Ab responses of the same magnitude as those induced after immunization with PA and the experimental adjuvants cholera toxin (CT). 3′3′-cGAMP also promotes serum anti-PA IgA and IgA-producing cells in the bone marrow. Furthermore, the saliva of mice immunized with 3′3′-cGAMP exhibits similar levels of PA-specific IgA Abs as groups immunized with CT as adjuvant. The adjuvant activity of 3′3′-cGAMP is associated with mixed Th1, Th2, and Th17 responses. 3′3′-cGAMP also induces rapid IFN-β and IL-10 responses in sublingual tissues and cervical lymph nodes, and TGF-β responses in the cervical lymph nodes, which can contribute to promoting IgA responses after sublingual immunization.^[1]

製品説明

3',3'-cGAMP (3',3'-cyclic GMP-AMP, Cyclic GMP-AMP, cGAMP) activates the endoplasmic reticulum (ER)-resident receptor stimulator of interferon genes (STING), thereby inducing an antiviral state and the secretion of type I IFNs.

in vivo

Sublingual immunization of mice with Bacillus anthracis protective antigen (PA) and the STING ligand 3′3′-cGAMP promotes PA-specific serum IgG Ab responses of the same magnitude as those induced after immunization with PA and the experimental adjuvants cholera toxin (CT). 3′3′-cGAMP also promotes serum anti-PA IgA and IgA-producing cells in the bone marrow. Furthermore, the saliva of mice immunized with 3′3′-cGAMP exhibits similar levels of PA-specific IgA Abs as groups immunized with CT as adjuvant. The adjuvant activity of 3′3′-cGAMP is associated with mixed Th1, Th2, and Th17 responses. 3′3′-cGAMP also induces rapid IFN-β and IL-10 responses in sublingual tissues and cervical lymph nodes, and TGF-β responses in the cervical lymph nodes, which can contribute to promoting IgA responses after sublingual immunization.^[1]

プロトコル(参考用のみ)

動物実験	動物モデル	9–12 week old female C57BL/6J mice
	投薬量	10 μg
	投与方法	Oral gavage

参考

https://pubmed.ncbi.nlm.nih.gov/28343781/

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。