3'-Hydroxypterostilbene

製品コードS3940 バッチS394001

印刷

化学情報

Chemical Structure Synonyms 3'-HPT Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C16H16O4
分子量 272.30 CAS No. 475231-21-1
Solubility (25°C)* 体外 DMSO 54 mg/mL (198.31 mM)
Ethanol 54 mg/mL (198.31 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 3'-Hydroxypterostilbene (3'-HPT) is one of the active constituents of Sphaerophysa salsula and Pterocarpus marsupium which may be useful in treating different types of haematological malignancies.
in vitro 3'-Hydroxypterostilbene decreases cell growth in cultured human colon cancer cells (COLO 205, HCT-116, and HT-29) in a dose-dependent manner, with IC50 values of 9.0, 40.2, and 70.9 µM, respectively. This compound effectively inhibits the growth of human colon cancer cells by inducing apoptosis and autophagy. Treatment with this compound causes reduction of mitochondrial membrane potential and induction of caspase-3 and caspage-9, which is associated with the degradation of DFF-45 and PARP, precedes the onset of apoptosis. It significantly down-regulates phosphatidylinositol 3-kinase (PI3K)/Akt and mitogen-activated protein kinases (MAPKs) signalings including decreased the phosphorylation of mammalian target of rapamycin (mTOR)[1].
in vivo 3'-Hydroxypterostilbene by i.p. injection has anti-tumor efficacy gainst colon cancer through the inhibition of inflammation, metastasis, and angiogenesis as well as through the induction of apoptosis[1].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 COLO 205 cells
濃度 5-100 µM
反応時間 24 h
実験の流れ

For sub-G1 cell population analysis, COLO 205 cells (2×105 cells/mL) are cultured in 12 well and treatment with various concentrations of pterostilbene or 3′-hydroxypterostilbene for 24 h. The cells are then harvested, washed with PBS, resuspended in 200 µL of PBS, and fixed in 800 µL of iced 100% ethanol at -20°C. After being left to stand overnight, the cell pellets are collected by centrifugation, resuspended in 1 mL of hypotonic buffer (0.5% Triton X-100 in PBS and 0.5 µg/mL RNase) with PI (50 µg/mL) and incubated at 37°C in the dark for 30 min. Fluorescence emitted from the PI-DNA complex is quantitated after excitation of the fluorescent dye.
動物実験 動物モデル nude mice bearing COLO 205 tumor xenografts
投薬量 10 mg/kg
投与方法 i.p.

参考

  • https://pubmed.ncbi.nlm.nih.gov/25389774/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Pterostilbene, a Dimethyl Derivative of Resveratrol, Exerts Cytotoxic Effects on Melanin-Producing Cells through Metabolic Activation by Tyrosinase [ Int J Mol Sci, 2024, 25(18)9990] PubMed: 39337478

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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