3-Nitropropionic acid (3-NP)

製品コードS3652 バッチS365203

印刷

化学情報

Synonyms

β-Nitropropionic Acid

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₃H₅NO₄

分子量

119.08

CAS No.

504-88-1

Solubility (25°C)*

体外

DMSO

24 mg/mL (201.54 mM)

Water

24 mg/mL (201.54 mM)

Ethanol

24 mg/mL (201.54 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	1.200mg/ml (10.08mM)	Taking the 1 mL working solution as an example, add 50 μL of 24 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.600mg/ml (5.04mM)	Taking the 1 mL working solution as an example, add 50 μL of 12 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	3-ニトロプロピオン酸（3-NP）は、ミトコンドリア呼吸複合体IIコハク酸脱水素酵素の不可逆的阻害剤であり、電子伝達系の破壊を通じてエネルギー枯渇を引き起こします。
in vitro	3-nitropropionic acid (3-NPA) is an irreversible inhibitor of complex II in the mitochondria. It impairs cellular energy metabolism via inhibition of succinate dehydrogenase, which induces a reduction in ATP production and leads to oxidative stress. 3-NPA can cause the generation and release of ROS from mitochondria, mitochondrial DNA damage, and thus loss of mitochondrial function. 3-NPA reduces the number of large follicles, impairs oocyte development, and increases the percentage of atretic large follicles and ROS levels in oocytes and granulosa cells. ROS production followed by mitochondrial DNA damage is the primary event in 3-NPA toxicity, and Bcl-2 protects PC12 cells from 3-NPA toxicity by preventing mitochondrial DNA damage.
in vivo	3-NPA induces granulosa cell apoptosis, large follicle atresia, and an increase of ROS levels in the ovary.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	PC12 cells
	濃度	4-8 mM
	反応時間	48 h
	実験の流れ	Viability of the PC12 cells with or without treatment of 3-NPA is estimated using trypan blue exclusion test. Cells are grown in Petri dishes to a density of 1 × 10⁶ cells/dish. After treatment with 4 or 8 mM 3-NPA for 48 h, cells are washed with Hank's balanced salt solution. Trypan blue (0.4 %) is added to the cells and incubated for 5 min. Both viable and nonviable cells are scored on a hemocytometer using a Nikon microscope. The nonviable cells are expressed as a percentage of total cell population
動物実験	動物モデル	ICR mice
	投薬量	6.25, 12.5 and 25 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/16371358/
https://pubmed.ncbi.nlm.nih.gov/24505260/
https://pubmed.ncbi.nlm.nih.gov/15710238/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

m6A-binding protein YTHDF2 alleviates 3-nitropropionic acid-induced mitochondrial dysfunction of goat granulosa cells [ Ecotoxicol Environ Saf, 2025, 304:119109]	PubMed: 41016146
Mitochondrial fumarate promotes ischemia/reperfusion-induced tubular injury [ Acta Physiol (Oxf), 2024, 240(4):e14121.]	PubMed: 38409944
Arachidonic Acid Metabolism Controls Macrophage Alternative Activation Through Regulating Oxidative Phosphorylation in PPARγ Dependent Manner [ Front Immunol, 2021, 12:618501]	PubMed: 34149684

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。