4-HNE (4-Hydroxynonenal)

製品コードS9793 バッチS979302

印刷

化学情報

Synonyms

HNE, 4-hydroxy Nonenal, 4-hydroxy-2-nonenal

Storage
(From the date of receipt)

2 years -20°C liquid

化学式

C₉H₁₆O₂

分子量

156.22

CAS No.

75899-68-2

Solubility (25°C)*

体外

DMSO

100 mg/mL (640.12 mM)

Ethanol

100 mg/mL (640.12 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution	5% DMSO 1 95% Corn oil	0.83mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 16.6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	5.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	4-HNE (4-Hydroxynonenal) is one of the major end products of lipid peroxidation and has been widely accepted as an inducer of oxidative stress.
in vitro	4-Hydroxynonenal (4-HNE) is one of the main end products of lipid peroxidation, which induces the expression of heme oxygenase-1 (HO-1), peroxiredoxin-1 and stress protein A170 in an Nrf2-dependent manner in murine macrophages.<sup><a class="sref" href="#s_ref">[2]</a></sup>
密度	0.9 g/mL

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Mouse Peritoneal Macrophages
	濃度	10 μM
	反応時間	24 h
	実験の流れ	Oil Red O Staining: Mouse peritoneal macrophages were incubated for 24 h at 37℃ in 5% CO2 in PRMI 1640 medium containing 10% FCS in the absence or presence of 4-Hydroxynonenal (4-HNE) (10 μM) or moxLDL or oxLDL (200 μg protein/ml). Cells were washed twice, fixed in formalin, and stained with oil red O. The area of lipid-loaded macrophages was measured using a computerized MacSCOPE image analysis system.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

IL-23 signaling prevents ferroptosis-driven renal immunopathology during candidiasis [ Nat Commun, 2022, 13(1):5545]	PubMed: 36138043

IL-23 signaling prevents ferroptosis-driven renal immunopathology during candidiasis [ Nat Commun, 2022, 13(1):5545]

PubMed: 36138043

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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