5-Fluoroorotic acid

製品コードS9873 バッチS987301

印刷

化学情報

Chemical Structure Synonyms 5-FOA, 5-Fluoroorotate, NSC 31712 Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C5H3FN2O4
分子量 174.09 CAS No. 703-95-7
Solubility (25°C)* 体外 DMSO 35 mg/mL (201.04 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 5-Fluoroorotic acid (5-FOA, 5-Fluoroorotate, NSC 31712) is a selective agent in yeast molecular genetics. 5-Fluoroorotic acid possesses a well-expressed anticandidal effect close to that of 5-fluorocytosine, as well as moderate antidermatophytal effects.
in vitro

5-Fluoroorotic acid (5-FOA) is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.[1]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Yeast cells
濃度 1 mg/ml, 500 μg/ml
反応時間 4-7 days
実験の流れ

Yeast cells are generally pregrown in liquid YPD medium or on YPD plates prior to selection on 5-Fluoroorotic acid. As many as 107 cells may be plated on a single, 50 mm Petri dish containing this compound medium. Resistant colonies will grow up within 4-7 days at 30°. The most useful concentration of the drug is generally 1 mg/ml of this chemical, but in the interest of economy it is possible to use as little as 500 μg/ml of this compound. When lower concentrations of this chemical are used, however, background growth of the Ura+ cells is considerably greater. This background growth is less of a problem when Ura- derivatives occur at a high frequency (e.g., when a URA3+ marker is located between the elements of a direct duplication). In some instances it may be desirable to use a rich medium such as YPD as opposed to the minimal medmm normally used with this compound selection.

参考

  • https://pubmed.ncbi.nlm.nih.gov/3323810/
  • https://pubmed.ncbi.nlm.nih.gov/3586772/

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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