Datasheet

生物学的記述

Specificity	5-hydroxymethylcytosine Antibody [B13H23] detects endogenous levels of total 5-hydroxymethylcytosine protein.
Background	5-hydroxymethylcytosine (5-hmC) is a distinct and significant epigenetic DNA modification resulting from the oxidation of 5-methylcytosine (5-mC) by the ten-eleven translocation (TET) family of dioxygenases (TET1, TET2, TET3). This modification adds a hydroxymethyl group at the 5-position of cytosine, altering the chemical landscape of DNA without disrupting base pairing, thereby influencing chromatin structure and gene regulation. 5-hmC differs from 5-mC by the presence of this hydroxymethyl group, which modulates the binding of methyl-CpG binding proteins (e.g., MeCP2) and recruits specialized reader proteins that mediate downstream effects. 5-hmC serves a dual role: as an intermediate in active DNA demethylation, where it undergoes further oxidation to 5-formylcytosine and 5-carboxylcytosine, followed by base excision repair, and as a stable epigenetic mark enriched in gene bodies, promoters, and enhancers, particularly in neuronal tissues. Its presence correlates with transcriptional activation by facilitating an open chromatin state and is dynamically regulated during development and in response to environmental cues. Aberrant 5-hmC patterns are implicated in tumorigenesis (e.g., gliomas) and neurological disorders such as Rett syndrome.

Specificity

5-hydroxymethylcytosine Antibody [B13H23] detects endogenous levels of total 5-hydroxymethylcytosine protein.

Background

5-hydroxymethylcytosine (5-hmC) is a distinct and significant epigenetic DNA modification resulting from the oxidation of 5-methylcytosine (5-mC) by the ten-eleven translocation (TET) family of dioxygenases (TET1, TET2, TET3). This modification adds a hydroxymethyl group at the 5-position of cytosine, altering the chemical landscape of DNA without disrupting base pairing, thereby influencing chromatin structure and gene regulation. 5-hmC differs from 5-mC by the presence of this hydroxymethyl group, which modulates the binding of methyl-CpG binding proteins (e.g., MeCP2) and recruits specialized reader proteins that mediate downstream effects. 5-hmC serves a dual role: as an intermediate in active DNA demethylation, where it undergoes further oxidation to 5-formylcytosine and 5-carboxylcytosine, followed by base excision repair, and as a stable epigenetic mark enriched in gene bodies, promoters, and enhancers, particularly in neuronal tissues. Its presence correlates with transcriptional activation by facilitating an open chromatin state and is dynamically regulated during development and in response to environmental cues. Aberrant 5-hmC patterns are implicated in tumorigenesis (e.g., gliomas) and neurological disorders such as Rett syndrome.

使用情報

Application

IHC, IF, FCM

Dilution

IHC	IF	FCM
1:2000-1:20000	1:1000-1:4000	1:400-1:2000

Reactivity

Source

Rabbit Monoclonal Antibody

MW

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/28499883/
https://pubmed.ncbi.nlm.nih.gov/28769976/

5-hydroxymethylcytosine Antibody [B13H23]

生物学的記述

使用情報

References

Application Data