6AN (6-Aminonicotinamide)

製品コードS9783 バッチS978302

印刷

化学情報

Chemical Structure Synonyms Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C6H7N3O
分子量 137.14 CAS No. 329-89-5
Solubility (25°C)* 体外 DMSO 27 mg/mL (196.87 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 6AN (6-Aminonicotinamide) is an antimetabolite used to inhibit the NADPH-producing pentose phosphate pathway (PPP) in many cellular systems, making them more susceptible to oxidative stress. 6-Aminonicotinamide is a competitive inhibitor of NADP+-dependent enzyme glucose-6-phosphate dehydrogenase (G6PD) with Ki of 0.46 μM.
in vitro

Blocking the pentose phosphate pathway (PPP) by 6-AN (6-Aminonicotinamide) significantly inhibits mES colony formation in the presence of a normal concentration of glucose. This compound effectively inhibits AKT phosphorylation and activation of its downstream effector S6. It has no effect on the ERK and PDK1 activities, suggesting an AKT-specific effect, and inhibits cell growth.[2]
in vivo

PTEN null human cancer cells and in vivo murine models are sensitive to 6-AN (anti-PPP, anti pentose phosphate pathway) treatments, suggesting the importance of the PPP in maintaining AKT activation even in the presence of a constitutively activated PI3K pathway. The treatment of TALL models with this compound significantly increases the Phlda3 mRNA and protein levels, accompanied by substantial decreases in the levels of P-AKT and P-S6 as well as the PPP metabolic intermediates.[2]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HeLa cells, mES cells
濃度 100 nM
反応時間 24 h, 2 days, 3 days, 4 days
実験の流れ

PHLDA3 knockout blocks the effects of 6-AN, R5P or uridine on cell growth in vitro. Isogenic PHLDA3 WT and knockout HeLa cells are seeded in 96-well plates at a density of 1000 cells/well. After 48 h, the cells are treated with this compound (100 nM). Cell viability is detected by CCK-8 assays at indicated time points.
動物実験 動物モデル male CAnN.Cg-Foxn1nu/CrlVr mice
投薬量 1 mg/kg, 15 mg/kg
投与方法 IP

参考

  • https://pubmed.ncbi.nlm.nih.gov/30170107/
  • https://pubmed.ncbi.nlm.nih.gov/32312982/

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長期の保管のために-20°Cの下で製品を保ってください。

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