7,8-Dihydroxyflavone

製品コードS8319 バッチS831901

印刷

化学情報

Chemical Structure Synonyms 7,8-DHF Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C15H10O4
分子量 254.24 CAS No. 38183-03-8
Solubility (25°C)* 体外 DMSO 50 mg/mL (196.66 mM)
Ethanol 1 mg/mL (3.93 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 7,8-Dihydroxyflavone (7,8-DHF) acts as a potent and selective small-molecule agonist of the TrkB receptor (Kd ≈ 320 nM), the main signaling receptor of brain-derived neurotrophic factor (BDNF).
in vitro 7,8-DHF is one of the positive compounds that specifically activate TrkB, but not TrkA or TrkC, at a concentration of 250 nM. In addition to cortical and hippocampal neurons, 7,8-DHF also protects other cell types including the RGC (retinal ganglion cells) and PC12 cells from excitotoxic and oxidative stress-induced apoptosis and cell death. Thus, it has neuroprotective properties[1].
in vivo 7,8-Dihydroxyflavone is a bioavailable chemical that can pass through the BBB to provoke TrkB and its downstream PI3K/Akt and MAPK activation in mouse brain upon intraperitoneal or oral administration. 7,8-DHF promotes the survival and reduces apoptosis in cortical neurons of traumatic brain injury as administration of 7,8-DHF at 3 h post-injury reduces brain tissue damage via the PI3K/Akt pathway. Its treatment does not induce any apparent toxicity in mice and is not toxic to the mice during the chronic treatment. 7,8-DHF displays robust therapeutic efficacy toward Alzheimer's disease and inhibits obesity through activating muscular TrkB[1].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Rat pheochromocytoma (PC12) cells
濃度 1-25 μM
反応時間 1 h
実験の流れ

PC12 cells are seeded in 96-well plates at 104/well. After pretreatment with 7,8-DHF (1-25 μM) for 1 h, the cells are exposed to 6-OHDA (100 μM) for subsequent 24 h. The PI3k inhibitor LY294002 or MEK inhibitor PD98059 is added 30 min before 7,8-DHF treatment. At the end of the experiment, PC12 cells are incubated with 20 μl of MTT solution (5 mg/ml in PBS) for 4 h at 37 ◦C. The dark blue formazan product due to the reduction of MTT is dissolved in 150 μl of DMSO, and the absorbance at 570 nm is recorded with a microplate reader. The viability is expressed as the percentage of the untreated control cells.
動物実験 動物モデル Male Wistar rats; male APPswe/PS1dE9 transgenic mice
投薬量 0.1 mg/kg, 0.3 mg/kg, 1 mg/kg and 3 mg/kg(Rats); 0.1 mg/kg(mice)
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Polyphyllin VII induces CTC anoikis to inhibit lung cancer metastasis through EGFR pathway regulation [ Int J Biol Sci, 2023, 19(16):5204-5217] PubMed: 37928267
Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD [ Pharmacol Res, 2022, 176:106079] PubMed: 35026406
Receptor-Independent Anti-Ferroptotic Activity of TrkB Modulators [ Int J Mol Sci, 2022, 23(24)16205] PubMed: 36555849
Reduced BDNF expression in the auditory cortex contributed to neonatal pain-induced hearing impairment and dendritic pruning deficiency in mice [ Reg Anesth Pain Med, 2022, rapm-2022-103621] PubMed: 36384877
Hippocampal neurogenesis interferes with extinction and reinstatement of methamphetamine-associated reward memory in mice [ Neuropharmacology, 2021, 196:108717] PubMed: 34273388
Role of the BDNF/TrkB/CREB signaling pathway in the cytotoxicity of bisphenol S in SK-N-SH cells [ J Biochem Mol Toxicol, 2021, e22775] PubMed: 33749030
Optimized integration of fluoxetine and 7, 8-dihydroxyflavone as an efficient therapy for reversing depressive-like behavior in mice during the perimenopausal period. [ Prog Neuropsychopharmacol Biol Psychiatry, 2020, 13;101:109939] PubMed: 32243998
Nephronectin Expression is Inhibited by Inorganic Phosphate in Osteoblasts [ Calcif Tissue Int, 2019, 104(2):201-206] PubMed: 30341591

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。