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Synonyms | Asaronaldehyde | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C10H12O4 |
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分子量 | 196.2 | CAS No. | 4460-86-0 | |
Solubility (25°C)* | 体外 | DMSO | 39 mg/mL (198.77 mM) | |
Ethanol | 16 mg/mL (81.54 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Asaraldehyde (Asaronaldehyde) is a natural COX-2 inhibitor, exhibiting 17-fold selectivity over COX-1. |
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in vitro | Asaraldehyde is found to be the most abundant constituent, but is totally absent in its cultured broth and its natural host, C. kanehirae wood. On feeding with the acid-digested sawdust of C. kanehirae wood or vanillin to the broth for culture, Asaraldehyde is produced in both cultured broths. [1] Asaraldehyde shows 3.32% of prostaglandin H endoperoxide synthase-1 (COX-1) inhibitory activity and 52.69% prostaglandin H endoperoxide synthase-2 (COX-2) inhibitory activity, respectively at 100 mg/mL. Asaraldehyde shows selectivity towards COX-2 enzyme inhibition at 100 µg/mL. The COX-2/COX-1 ratio for Asaraldehyde is 17.68 at 100 µg/mL compared to solvent control. [2] Asaraldehyde down-regulates C/EBPβ, C/EBPδ, and C/EBPα. Asaraldehyde suppresses expression of PPARγ1 and PPARγ2. Asaraldehyde also significantly suppresses the expression of acetyl-CoA carboxylase (ACC). [2] |
細胞アッセイ | 細胞株 | 3T3-L1 cells |
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濃度 | 100 μg/mL | |
反応時間 | 24 hours | |
実験の流れ | 3T3-L1 cells are seeded in 96-well plates at a concentration of 104 /well. Twenty-four hours after seeding, the cells are treated with 100 μg/mL of Asaraldehyde for 24 hours or for the whole 8-day differentiation period. Fully differentiated adipocytes are also treated with 100 μg/mL of Asaraldehyde for 24 hours-72 hours to test the cytotoxicity. At the end of treatment, cells are cultured with MTT at a final concentration of 0.5 mg/mL for another 4 hours. The purple MTT formazan is dissolved by DMSO and the absorbance at 570 nm is taken with a spectrophotometer. The absorbance is proportional to the viability of adipocytes. |
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長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。