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受注：045-509-1970 |
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化学情報
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Synonyms | NSC 241240, JM-8, CBDCA | Storage (From the date of receipt) |
2 years 4°C(in the dark) powder |
| 化学式 | C6H12N2O4Pt |
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| 分子量 | 371.25 | CAS No. | 41575-94-4 | |
| Solubility (25°C)* | 体外 | Water | 3.2 mg/mL warmed with 50ºC water bath (8.61 mM) | |
| Ethanol | Insoluble | |||
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
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溶剤液（一定の濃度）を調合する
生物活性
| 製品説明 | Carboplatin is a DNA synthesis inhibitor by binding to DNA and interfering with the cell's repair mechanism in A2780, SKOV-3, IGROV-1, and HX62 cells.Solutions are unstable and should be fresh-prepared.DMSO is not recommended to dissolve platinum-based drugs, which can easily lead to drug inactivation. |
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| in vitro | Carboplatin exhibits an inhibitory effect on cell proliferation in a human ovarian cancer cell line panel, including A2780, SKOV3, and IGROV-1 cells with IC50 of 6.1 μM, 12.4 μM and 2.2 μM, respectively. [1] Carboplatin also show the anti-proliferative activities in lung carcinoid cell line, such as UMC-11, H727, and H835 cells with IC50 of 36.4 μM, 3.4 μM and 35.8 μM, respectively. [2] |
| in vivo | In A2780 tumor xenografts, Carboplatin (60 mg/kg via i.p.) given as single agents shows a modest antitumor effect with the relative tumor volumes on day 6 of 8.4 compared to the control of 11.9, and the day 6 tumor weights relative to control (T/C) of 67%. [1] For the VC8 (Brca2-deficient) xenografts, Carboplatin treatment delays tumor growth and reduces tumor mass by 42% compared to the vehicle group. [3] |
| 特徴 | This product is not recommended to be dissolved in dimethylsulfoxide (DMSO).[6] |
プロトコル(参考用のみ)
| 細胞アッセイ | 細胞株 | A2780, SKOV3, IGROV-1 and HX62 |
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| 濃度 | 0-200 μM | |
| 反応時間 | 72 hours | |
| 実験の流れ | 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assays: Exponentially growing A2780, SKOV3, IGROV-1 and HX62 ovarian cancer cells are plated in 96 well plates. A range of drug concentrations are added and the plates are incubated for 72 hours to allow for 3–4 doubling times. Each experiment is carried out in triplicate. Sulforhodamine B (SRB) assays: Exponentially growing A2780 cells are plated in 96 well microtitre plates. For experiments studying concomitant exposure, cells are exposed to increasing concentrations of both drugs for 96 hours. For experiments studying the effect of sequence of exposure to 17-AAG or carboplatin cells are exposed to increasing concentrations of 17-AAG or carboplatin for 24 hours. A period of 24-hour exposure to the first agent is chosen so that the A2780 cells would be exposed to the first drug for at least one doubling time (18-24 hours). The cells are then washed with sterile phosphate buffered saline and the medium is replenished. Following this, the second drug (to which the cells are not exposed to in the first 24 hours) or medium is added for 96 hours. All experiments are carried out in triplicate. The results of combination studies are analyzed using the well-established principles of median effect analysis method. The effects of the combination are calculated using an in-house spreadsheet. |
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| 動物実験 | 動物モデル | The A2780 human ovarian cancer cell line is grown as a subcutaneous xenograft in female athymic NCr nude mice (nu/nu) in each flank. |
| 投薬量 | ≤60 mg/kg (When Carboplatin is dissolved in sodium chloride solution, it will induce physio-chemical and pharmacokinetic changes.) | |
| 投与方法 | Administered via i.p. |
参考
カスタマーフィードバック
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Data from [PLoS One, 2012, 8, e72637]
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Data independently produced by , , Dr. Helen Sadik of Johns Hopkins University
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Data independently produced by , , Dr. Helen Sadik of Johns Hopkins University
Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：
| FAK Drives Resistance to Therapy in HPV-Negative Head and Neck Cancer in a p53-Dependent Manner [ Clin Cancer Res, 2024, 30(1):187-197] | PubMed: 37819945 |
| Synergistic antitumor activity between HER2 antibody-drug conjugate and chemotherapy for treating advanced colorectal cancer [ Cell Death Dis, 2024, 15(3):187] | PubMed: 38443386 |
| Human Fallopian Tube-Derived Organoids with TP53 and RAD51D Mutations Recapitulate an Early Stage High-Grade Serous Ovarian Cancer Phenotype In Vitro [ Int J Mol Sci, 2024, 25(2)886] | PubMed: 38255960 |
| Engineered MED12 mutations drive leiomyoma-like transcriptional and metabolic programs by altering the 3D genome compartmentalization [ Nat Commun, 2023, 14(1):4057] | PubMed: 37429859 |
| Engineered MED12 mutations drive leiomyoma-like transcriptional and metabolic programs by altering the 3D genome compartmentalization [ Nat Commun, 2023, 14(1):4057] | PubMed: 37429859 |
| Low-dose carboplatin modifies the tumor microenvironment to augment CAR T cell efficacy in human prostate cancer models [ Nat Commun, 2023, 14(1):5346] | PubMed: 37660083 |
| Low-dose carboplatin modifies the tumor microenvironment to augment CAR T cell efficacy in human prostate cancer models [ Nat Commun, 2023, 14(1):5346] | PubMed: 37660083 |
| Patient- and xenograft-derived organoids recapitulate pediatric brain tumor features and patient treatments [ EMBO Mol Med, 2023, 15(12):e18199] | PubMed: 38037472 |
| HLF promotes ovarian cancer progression and chemoresistance via regulating Hippo signaling pathway [ Cell Death Dis, 2023, 14(9):606] | PubMed: 37709768 |
| HLF promotes ovarian cancer progression and chemoresistance via regulating Hippo signaling pathway [ Cell Death Dis, 2023, 10.1038/s41419-023-06076-5] | PubMed: 37709768 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
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