Chloramphenicol

製品コードS1677 バッチS167702

印刷

化学情報

Chemical Structure Synonyms Chloromycetin Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C11H12Cl2N2O5
分子量 323.13 CAS No. 56-75-7
Solubility (25°C)* 体外 DMSO 120 mg/mL (371.36 mM)
Ethanol 65 mg/mL (201.15 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 3.250mg/ml (10.06mM) Taking the 1 mL working solution as an example, add 50 μL of 65 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 0.540mg/ml (1.67mM) Taking the 1 mL working solution as an example, add 50 μL of 10.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Chloramphenicol (Chloromycetin) is a bacteriostatic by inhibiting protein synthesis.
in vitro Chloramphenicol results in a constitutive increase in the level of cspA transcript and constitutive production of CS7.4. [1] This compound down-regulates the mitochondrial DNA-encoded Cox I protein, but not nuclear-encoded proteins. It increases cellular levels of the p21(waf1/cip1) protein and p21(waf1/cip1) mRNA through a p53-independent pathway. [2] This chemical, at concentrations greater than 0.1 g/L (0.3 mM), inhibits the denitrifying enzyme activity (DEA) of slurries of humisol and sandy loam soils by disrupting the activity of existing nitrate reductase enzymes. [3] It, at concentrations from 5 mM to 2 mM initiates apoptosis in dividing cells from a monkey kidney-derived cell line and in haematopoietic progenitor cells from human neonatal cord blood. This compound suppresses growth of progenitor cells. [4]
in vivo Chloramphenicol results in increased t1/2(beta) (by 209%), and decreased ClB (by 45%), and prolonged recovery indices (by 768 to 946%) in greyhounds. [5] This compound suppresses increases in the body weight and liver weight of experimental animals and at the same time induced a remarkable increase in lipid peroxidation in the liver during the formation of megamitochondria. This chemical-induced formation of megamitochondria is not simply ascribed to the suppression of the dividing process of mitochondria due to lowered protein synthesis in mitochondria but is intimately related to oxidative stress. [6]

プロトコル(参考用のみ)

参考

  • https://pubmed.ncbi.nlm.nih.gov/8376329/
  • https://pubmed.ncbi.nlm.nih.gov/15905168/
  • https://pubmed.ncbi.nlm.nih.gov/10427039/
  • https://pubmed.ncbi.nlm.nih.gov/7695156/
  • https://pubmed.ncbi.nlm.nih.gov/9186547/
  • https://pubmed.ncbi.nlm.nih.gov/8685919/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

The mitochondrial protein ERAL1 suppresses RNA virus infection by facilitating RIG-I-like receptor signaling [ Cell Rep, 2021, 34(3):108631] PubMed: 33472079
The mTORC1-eIF4F axis controls paused pluripotency [ EMBO Rep, 2021, e53081] PubMed: 34866316
Twenty-seven-nucleotide repeat insertion in the rplV gene confers specific resistance to macrolide antibiotics in Staphylococcus aureus [ Oncotarget, 2018, 9(40):26086-26095] PubMed: 29899844

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。