Dexamethasone

製品コードS1322 バッチS132204

化学情報

	Synonyms	NSC 34521, Hexadecadrol, Prednisolone F,MK-125	Storage (From the date of receipt)	powder, 4°C (in the dark) ; in solvent，-80°C (in the dark)
	化学式	C₂₂H₂₉FO₅
	分子量	392.46	CAS No.	50-02-2
Solubility (25°C)*	体外	DMSO	78 mg/mL (198.74 mM)
		Ethanol	11 mg/mL (28.02 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

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生物活性

製品説明	Dexamethasone is a potent synthetic member of the glucocorticoid class of steroid drugs, and an interleukin receptor modulator that has anti-inflammatory and immunosuppressant effects. Dexamethasone induces autophagy and mitophagy. Dexamethasone is tested in hospitalized patients with COVID-19 and is found to have benefits for critically ill patients.
in vitro	Dexamethasone results in decrease in transmonolayer paracellular permeability mainly to sucrose, fluorescein and dextrans of up to 20 KDa in an immortalised rat brain endothelial cell line (GPNT). Dexamethasone results in filamentous actin and the cytoskeleton associated protein cortactin being highly concentrated in the regions of cell-cell contact with few F-actin stress fibres visible within the cytoplasm in cultured rat brain endothelial cells, an observation consistent with a more differentiated barrier phenotype induced by dexamethasone. Dexamethasone treatment has been shown to strongly stimulate the level of the Id-1 protein, which is a serum-inducible helix-loop-helix transcriptional repressor, involved in cell differentiation, and this effect was shown to be associated with reorganisation of ZO-1 to the cell periphery in Con8 mammary epithelial tumor cells. Dexamethasone prevents cytokine-induced enhanced expression of MMP-9 and alterations in the expression of ZO-1 in untreated GPNT monolayers. ^[1] Dexamethasone depletes both basal and TNF-alpha-stimulated GSH levels by down-regulating the gamma-GCS-heavy subunit transcription via a mechanism involving AP-1 (c-Jun) in alveolar epithelial cells. Dexamethasone decreases both basal and stimulated GSH levels (TNF-α-treated) in alveolar epithelial cells (A549), without any change in GSSG. ^[2]
in vivo	Dexamethasone is administered i.m. to pregnant ewes, leads to the following results (1) blood pressure is unchanged; (2) as previously reported in the fetus, sensitivity to endothelin-1 (ET) is increased; (3) acetylcholine-induced relaxation is increased; (4) L-NAME suppressible vasodilatory response to ET is abolished; (5) there is no change in endothelium-independent vasodilatation; and (6) there is no change in eNOS RNA and protein levels, when compared to saline treated controls. ^[3]

製品説明

Dexamethasone is a potent synthetic member of the glucocorticoid class of steroid drugs, and an interleukin receptor modulator that has anti-inflammatory and immunosuppressant effects. Dexamethasone induces autophagy and mitophagy. Dexamethasone is tested in hospitalized patients with COVID-19 and is found to have benefits for critically ill patients.

in vitro

Dexamethasone results in decrease in transmonolayer paracellular permeability mainly to sucrose, fluorescein and dextrans of up to 20 KDa in an immortalised rat brain endothelial cell line (GPNT). Dexamethasone results in filamentous actin and the cytoskeleton associated protein cortactin being highly concentrated in the regions of cell-cell contact with few F-actin stress fibres visible within the cytoplasm in cultured rat brain endothelial cells, an observation consistent with a more differentiated barrier phenotype induced by dexamethasone. Dexamethasone treatment has been shown to strongly stimulate the level of the Id-1 protein, which is a serum-inducible helix-loop-helix transcriptional repressor, involved in cell differentiation, and this effect was shown to be associated with reorganisation of ZO-1 to the cell periphery in Con8 mammary epithelial tumor cells. Dexamethasone prevents cytokine-induced enhanced expression of MMP-9 and alterations in the expression of ZO-1 in untreated GPNT monolayers. ^[1]

Dexamethasone depletes both basal and TNF-alpha-stimulated GSH levels by down-regulating the gamma-GCS-heavy subunit transcription via a mechanism involving AP-1 (c-Jun) in alveolar epithelial cells. Dexamethasone decreases both basal and stimulated GSH levels (TNF-α-treated) in alveolar epithelial cells (A549), without any change in GSSG. ^[2]

in vivo

Dexamethasone is administered i.m. to pregnant ewes, leads to the following results (1) blood pressure is unchanged; (2) as previously reported in the fetus, sensitivity to endothelin-1 (ET) is increased; (3) acetylcholine-induced relaxation is increased; (4) L-NAME suppressible vasodilatory response to ET is abolished; (5) there is no change in endothelium-independent vasodilatation; and (6) there is no change in eNOS RNA and protein levels, when compared to saline treated controls. ^[3]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	CB CD34+ cells
	濃度	1 μM
	反応時間	16 h
	実験の流れ	Cells were treated with indicated concentrations of drug.
動物実験	動物モデル	Mice
	投薬量	1 mg/kg
	投与方法	i.p.

参考

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カスタマーフィードバック

: Data from [Oncogene, 2013, 32, 1316-29]

: Data from [Oncogene, 2013, 32, 1316-29]

: Data from [Data independently produced by , , Int J Oncol, 2018, doi:10.3892/ijo.2018.4635]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Virological characteristics of the SARS-CoV-2 BA.2.86 variant [ Cell Host Microbe, 2024, S1931-3128(24)00005-2]	PubMed: 38280382
Virological characteristics of the SARS-CoV-2 Omicron XBB.1.5 variant [ Nat Commun, 2024, 15(1):1176]	PubMed: 38332154
Glucocorticoid treatment influences prostate cancer cell growth and the tumor microenvironment via altered glucocorticoid receptor signaling in prostate fibroblasts [ Oncogene, 2024, 43(4):235-247]	PubMed: 38017134
β-catenin inhibition disrupts the homeostasis of osteogenic/adipogenic differentiation leading to the development of glucocorticoid-induced osteonecrosis of the femoral head [ Elife, 2024, 12RP92469]	PubMed: 38376133
Inhibition of PTPRE suppresses tumor progression and improves sorafenib response in hepatocellular carcinoma [ Biomed Pharmacother, 2024, 173:116366]	PubMed: 38458013
Kupffer cells abrogate homing and repopulation of allogeneic hepatic progenitors in injured liver site [ Stem Cell Res Ther, 2024, 15(1):48]	PubMed: 38378583
Serine to proline mutation at position 341 of MYOC impairs trabecular meshwork function by causing autophagy deregulation [ Cell Death Discov, 2024, 10(1):21]	PubMed: 38212635
The ESCRT protein CHMP5 promotes T cell leukemia by controlling BRD4-p300-dependent transcription [ bioRxiv, 2024, 2024.01.29.577409]	PubMed: 38352301
LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexia [ J Hematol Oncol, 2023, 16(1):30]	PubMed: 36973755
Bovine blastocyst like structures derived from stem cell cultures [ Cell Stem Cell, 2023, VOLUME 30, ISSUE 5,-P611-616.E7]	PubMed: None

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人間や獣医の診断であるか治療的な使用のためにでない。

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