Epirubicin HCl

製品コードS1223 バッチS122306

印刷

化学情報

Chemical Structure Synonyms IMI 28 HCl, NSC 256942 HCl, 4'-epidoxorubicin HCl Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C27H29NO11.HCl
分子量 579.98 CAS No. 56390-09-1
Solubility (25°C)* 体外 DMSO 100 mg/mL (172.41 mM)
Water (warmed with 50ºC water bath) 100 mg/mL (172.41 mM)
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 ドキソルビシンの半合成L-アラビノ誘導体であるEpirubicin HClは、Topoisomeraseを阻害することにより抗腫瘍作用を示します。Epirubicinはapoptosisを誘発します。
in vitro

Epirubicin, like doxorubicin, exerts its antitumor effects by complex with DNA, resulting in damage to DNA and interference with the synthesis of DNA, RNA, and proteins. Epirubicin may also affect the integrity and activity of cellular membranes. Maximal cell kill caused by Epirubicin occurs during the S phase of the cell cycle. With higher concentrations effects are also seen in early G2 as well as G1 and M phases. Epirubicin display antineoplastic activity against most cancer cells. Epirubicin is cytotoxic to Hepatoma G2 cells with IC50 of 1.6 μg/mL at 24hr. 1.6 μg/mL Epirubicin induces apoptosis of Hep G2 cells, and higher activity of catalase by 50%, Se-dependent glutathione peroxidase by 110%, Cu, Zn-superoxide dismutase by 172% and Mn-superoxide dismutase by 135%. Epirbicin increases the cellular expression of NADPH-CYP 450 reductase, and reduces GST-π expression.

in vivo

Epirubicin are clinically active against a broad range of tumor types, including breast cancer, malignant lymphomas, soft tissue sarcomas, lung cancer, pleural mesothelioma, gastrointestinal cancer, head and neck cancer, ovarian cancer, prostatic carcinoma, transitional bladder carcinoma and so on. Epirubicin at a dose of 3.5 mg/kg suppresses tumor mass of human breast tumor xenograft R-27 by 74.4 %.

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Human hepatocellular carcinoma cell Hep G2
濃度 0.05-12 μg/mL
反応時間 1 days
実験の流れ

Hep G2 cells (500 cells/well, monolayer) are plated in a 96-well plate. The next day the cells are treated with Epirubicin in the medium. At the end of the incubation periods, 15% volume of MTT dye solution is added. After 1 hr of incubation at 37℃, an equal volume of solubilization/stop solution (dimethylsul-foxide) is added to each well for an additional 1 hr incubation. The absorbance of the reaction solution at 570 nm is recorded.
動物実験 動物モデル Human breast tumor xenograft R-27
投薬量 3.5 mg/kg
投与方法 i.v. every 4 day for 3 times

参考

  • https://pubmed.ncbi.nlm.nih.gov/3005521/
  • https://pubmed.ncbi.nlm.nih.gov/15520498/
  • https://pubmed.ncbi.nlm.nih.gov/8353070/
  • https://pubmed.ncbi.nlm.nih.gov/9821299/

カスタマーフィードバック

, , Dr. Edita Aksamitiene from Thomas Jefferson University

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Combined Treatment of Tanshinone I and Epirubicin Revealed Enhanced Inhibition of Hepatocellular Carcinoma by Targeting PI3K/AKT/HIF-1α [ Drug Design, Development and Therapy, September 19, 2022, 3197-3213] PubMed: 36158238
Regularized Single-cell Imaging Enables Generalizable AI models for Stain-free Cell Viability Screening [ bioRxiv, September 11, 2025, 675708] PubMed: 41574984
A novel twelve-gene signature to predict neoadjuvant chemotherapy response and prognosis in breast cancer [ Frontiers in Immunology, October 19, 2022, 1035667] PubMed: 36341435
Anti-Tumor Effect of Apatinib and Relevant Mechanisms in Liposarcoma [ Frontiers in Oncology, November 17, 2021, 755553] PubMed: 34868934
CRISPRi/a Screens in Human iPSC-Derived Cardiomyocytes Identify Glycolytic Activation as a Druggable target for Doxorubicin-Induced Cardiotoxicity [ Cell Stem Cell, November 07, 2024, 1760-1776.e9]
Hedgehog signaling induces PD-L1 expression and tumor cell proliferation in gastric cancer [ Oncotarget, December 21, 2018, 37439-37457] PubMed: 30647844
Heterogeneous therapy-resistant cancer cells have distinct and exploitable drug sensitivity profiles [ bioRxiv, April 25, 2025, 2025.04.25.650475] PubMed: 40654745
FGD3 mediates lytic cell death, enhancing efficacy and immunogenicity of chemotherapy agents in breast cancer [ J Exp Clin Cancer Res, 2025, 44(1):299] PubMed: 41225536
Multi-layer stratified oncology platform utilizing transcriptomics, prostate cancer organoids, and modeling of drug response [ J Exp Clin Cancer Res, 2025, 44(1):290] PubMed: 41094672
Platinum nanoparticles interact with epirubicin in size dependent manner and affect its biological activity [ Eur J Pharm Biopharm, 2025, 216:114866] PubMed: 40939769

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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