Fisetin

製品コードS2298 バッチS229806

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C15H10O6
分子量 286.24 CAS No. 528-48-3
Solubility (25°C)* 体外 DMSO 57 mg/mL (199.13 mM)
Ethanol 9 mg/mL (31.44 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Fisetin (Fustel) is a potent sirtuin activating compound (STAC) and an agent that modulates sirtuins.
in vitro

Fisetin selectively kills SPiDER-β-Gal positive senescence neural cells in vitro.[2]
in vivo

Oral administration of fisetin, a senolytic drug, reduces the number of senescent neural cells observed, the SASP expression level, and depressive behavior in MRL/lpr mice.[2]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells
濃度 5, 10, or 20 μM
反応時間 48 h
実験の流れ

Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells are seeded on a 96-well black/clear bottom plate at 40,000 cells and 10,000 cells per well, respectively. After senescence induction for 6 days, 5 μM, 10 μM, or 20 μM fisetin is added and the cells are incubated for 48 h. Cell number and cellular senescence are determined by DAPI staining and SPiDER-β-Gal staining, respectively. Cells are washed twice with PBS, fixed in 4% paraformaldehyde at room temperature for 5 min, and washed twice with PBS. Sections are incubated in 20 M SPiDER-β-gal in solution in McIlvaine buffer (pH 6.0) for 60 min at 37 ℃. After washing of tissue sections, nuclei are stained with DAPI. Cells are observed. 
動物実験 動物モデル Female MRL/lpr mice, haplotype-matched female MRL/MPJ mice
投薬量 100 mg/kg
投与方法 p.o.

参考

  • https://pubmed.ncbi.nlm.nih.gov/19075764/
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8597709/

カスタマーフィードバック

Data from [Data independently produced by , , Alcohol Clin Exp Res, 2016, 40(10):2076-2084.]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Single-cell morphology encodes functional subtypes of senescence in aging human dermal fibroblasts [ Sci Adv, 2025, 11(17):eads1875] PubMed: 40279419
Pharmacological targeting of P300/CBP reveals EWS::FLI1-mediated senescence evasion in Ewing sarcoma [ Mol Cancer, 2024, 23(1):222] PubMed: 39367409
TAGLN2 induces resistance signature ISGs by activating AKT-YBX1 signal with dual pathways and mediates the IFN-related DNA damage resistance in gastric cancer [ Cell Death Dis, 2024, 15(8):608] PubMed: 39168971
SARS-CoV-2 spike-induced syncytia are senescent and contribute to exacerbated heart failure [ PLoS Pathog, 2024, 20(8):e1012291] PubMed: 39102426
Senolytic intervention improves cognition, metabolism, and adiposity in female APPNL-F/NL-F mice [ Geroscience, 2024, 10.1007/s11357-024-01308-8] PubMed: 39120687
The Occurrence of Senescence in the Arteriovenous Fistula in the Rat [ Kidney360, 2024, 6(1):27-37] PubMed: 39418108
Single-cell morphology encodes functional subtypes of senescence in aging human dermal fibroblasts [ bioRxiv, 2024, 2024.05.10.593637] PubMed: 38798365
Clearance of defective muscle stem cells by senolytics restores myogenesis in myotonic dystrophy type 1 [ Nat Commun, 2023, 14(1):4033] PubMed: 37468473
Fisetin ameliorates fibrotic kidney disease in mice via inhibiting ACSL4-mediated tubular ferroptosis [ Acta Pharmacol Sin, 2023, 10.1038/s41401-023-01156-w] PubMed: 37696989
Tubular cell senescence promotes maladaptive kidney repair and chronic kidney disease after cisplatin nephrotoxicity [ JCI Insight, 2023, 8(8)e166643] PubMed: 36917180

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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