Gemcitabine HCl

製品コードS1149 バッチS114910

化学情報

	Synonyms	NSC 613327 HCl,LY-188011 HCl	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₉H₁₁F₂N₃O₄.HCI
	分子量	299.66	CAS No.	122111-03-9
Solubility (25°C)*	体外	Water	59 mg/mL (196.88 mM)
		DMSO	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	ゲムシタビン塩酸塩 (Gemcitabine (LY-188011, NSC 613327) HCl) は DNA 合成阻害剤であり、 PANC1 細胞、 MIAPaCa2 細胞、 BxPC3 細胞および Capan2 細胞における IC50 はそれぞれ 50 nM, 40 nM, 18nM, 12 nM です。
in vitro	Gemcitabine induced NF-κB activity in BxPC-3, PANC-1, and MIA PaCa-2 cells and decreased the level of the NF-κB inhibitor IκBα in BxPC-3 and PANC-1 cells. Treatment of BxPC-3 cells with low dose Gemcitabine for 48 hours results in a dose-dependent increase in NF-κB binding. In contrast, NF-κB DNA binding is decreased in BxPC-3 cells treated with the higher Gemcitabine doses for 48 h; however, 24-h treatment with these higher doses increases NF-κB binding in BxPC-3 cells ^[2]
in vivo	Intratumoral NF-κB activity is significantly elevated (1.3- to 1.8-fold) in the Gemcitabine-treated mice compared to the PBS-treated mice, suggesting that Gemcitabine also induces NF-κB activation. ^[2]
特徴	Gemcitabine has been used to treat pancreatic cancer and has demonstrated effective anti-tumor activity.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	BxPC-3, MIA PaCa-2, and PANC-1 cells
	濃度	0.2 μM
	反応時間	24 hours or 48 hours
	実験の流れ	BxPC-3, MIA PaCa-2, and PANC-1 cells are seeded in a 96-well plate. After 24 hours, cells are treated with vehicle, DMAPT and/or Gemcitabine for an additional 24 hours or 48 hours. Apoptosis is quantified using the Cell Death Detection ELISA to detect the amount of cytoplasmic histone-associated DNA fragments and expressed relative to vehicle-treated cells.
動物実験	動物モデル	Athymic nude mice with MIA PaCa-2 cells
	投薬量	50 mg/kg or 100 mg/kg
	投与方法	Administered via i.p.

参考

カスタマーフィードバック

: Data from [Data independently produced by Nucleic Acids Res, 2014, 42(10), 6436-47]

: Data from [Cancer Immunol Immunother, 2013, 62, 383–391]

: Data independently produced by , , Dr. Helen Sadik of Johns Hopkins University

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

UPP1 enhances bladder cancer progression and gemcitabine resistance through AKT [ Int J Biol Sci, 2024, 20(4):1389-1409]	PubMed: 38385072
A novel DDIT3 activator dehydroevodiamine effectively inhibits tumor growth and tumor cell stemness in pancreatic cancer [ Phytomedicine, 2024, 155377]	PubMed: none
Gemcitabine Modulates HLA-I Regulation to Improve Tumor Antigen Presentation by Pancreatic Cancer Cells [ Int J Mol Sci, 2024, 25(6)3211]	PubMed: 38542184
Targeting of oncogenic AAA-ATPase TRIP13 reduces progression of pancreatic ductal adenocarcinoma [ Neoplasia, 2024, 47:100951]	PubMed: 38039923
Cell membrane-camouflaged bufalin targets NOD2 and overcomes multidrug resistance in pancreatic cancer [ Drug Resist Updat, 2023, 71:101005]	PubMed: 37647746
Pancreatic cancer cells upregulate LPAR4 in response to isolation stress to promote an ECM-enriched niche and support tumour initiation [ Nat Cell Biol, 2023, 25(2):309-322]	PubMed: 36646789
Analysis and modeling of cancer drug responses using cell cycle phase-specific rate effects [ Nat Commun, 2023, 14(1):3450]	PubMed: 37301933
A living ex vivo platform for functional, personalized brain cancer diagnosis [ Cell Rep Med, 2023, S2666-3791(23)00156-8]	PubMed: 37192626
Quantifying heterogeneity to drug response in cancer-stroma kinetics [ Proc Natl Acad Sci U S A, 2023, 120(11):e2122352120]	PubMed: 36897966
Therapeutic efficacy of a MMAE-based anti-DR5 drug conjugate Oba01 in preclinical models of pancreatic cancer [ Cell Death Dis, 2023, 14(4):295]	PubMed: 37120688

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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