Genistein

製品コードS1342 バッチS134202

印刷

化学情報

Chemical Structure Synonyms NPI 031L Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C15H10O5
分子量 270.24 CAS No. 446-72-0
Solubility (25°C)* 体外 DMSO 54 mg/mL (199.82 mM)
Ethanol 4 mg/mL (14.8 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 2.700mg/ml (9.99mM) Taking the 1 mL working solution as an example, add 50 μL of 54 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 0.340mg/ml (1.26mM) Taking the 1 mL working solution as an example, add 50 μL of 6.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Genistein, a phytoestrogen found in soy products, is a highly specific inhibitor of protein tyrosine kinase (PTK) which blocks the mitogenic effect mediated by EGF on NIH-3T3 cells with IC50 of 12μM.
in vitro

Genistein is an ATP competitive inhibitor. This compound inhibits tyrosine phosphorylation in isolated enzyme and receptor preparations and in whole cells including platelets, lymphocytes and a variety of cultured cells. It also inhibits EGF-stimulated phosphorylation in cultured cells as well as inhibition of Topo II (topoisomerase II). This chemical inhibits EGF-stimulated tyrosine phosphorylation in cultured A431 epidermoid carcinoma cells. Inhibition is competitive with ATP and noncompetitive with substrate. [1] It blocks the mitogenic effect mediated by EGF and thrombin on NIH-3T3 cells.[2] This compound also acts as an agonist at the GPR30 receptor and binds to PPARγ and estrogen receptors. It also binds to PPARγ, acting as an agonist at this receptor with Ki of 5.7 μM. [3]
in vivo

Genistein has chemopreventive effects on breast, prostate, and other endocrine-dependent tumors in adult animals. This compound in the diet reduced the incidence of poorly differentiated prostatic adenocarcinomas in a dose-dependent manner and down-regulated androgen receptor, estrogen receptor-alpha, epidermal growth factor receptor, and extracellular signal-regulated kinase-1 but not estrogen receptor-beta and transforming growth factor-alpha mRNA expressions. Dietary genistein protects against mammary and prostate cancers by regulating specific sex steroid receptors and growth factor signaling pathways.[4] This chemical combined with prostate tumor irradiation causes greater inhibition of primary tumor growth and increases control of spontaneous metastasis to para-aortic lymph nodes, increasing mouse survival. Paradoxically, treatment with this compound alone increases metastasis to lymph nodes. [5]

プロトコル(参考用のみ)

参考

  • https://pubmed.ncbi.nlm.nih.gov/3106339/
  • https://pubmed.ncbi.nlm.nih.gov/2153378/
  • https://pubmed.ncbi.nlm.nih.gov/12421816/
  • https://pubmed.ncbi.nlm.nih.gov/11880592/
  • https://pubmed.ncbi.nlm.nih.gov/16808622/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

HVJ-E links Apolipoprotein d to antitumor effects [ J Immunother Cancer, 2025, 13(6)e011442] PubMed: 40541272
Merlin controls limb development and thumb formation by regulating primary cilium-hedgehog signaling [ Cell Rep, 2025, 44(6):115849] PubMed: 40503937
Complex IIa formation and ABC transporters determine sensitivity of OSCC to Smac mimetics [ Cell Death Dis, 2024, 15(11):855] PubMed: 39578442
Patient-derived rhabdomyosarcoma cells recapitulate the genetic and transcriptomic landscapes of primary tumors [ iScience, 2024, 27(10):110862] PubMed: 39319271
Treatment of Ulcerative Colitis by Cationic Liposome Delivered NLRP3 siRNA [ Int J Nanomedicine, 2023, 18:4647-4662] PubMed: 37605735
Genistein Inhibits Proliferation and Metastasis in Human Cervical Cancer Cells through the Focal Adhesion Kinase Signaling Pathway: A Network Pharmacology-Based In Vitro Study in HeLa Cells [ Molecules, 2023, 28(4)1919] PubMed: 36838908
Evaluation of the liver targeting and anti‑liver cancer activity of artesunate‑loaded and glycyrrhetinic acid‑coated nanoparticles [ Exp Ther Med, 2023, 26(5):516] PubMed: 37854499
Evaluation of the liver targeting and anti‑liver cancer activity of artesunate‑loaded and glycyrrhetinic acid‑coated nanoparticles [ Exp Ther Med, 2023, 26(5):516] PubMed: 37854499
Polyaniline-Based Glyco-Condensation on Au Nanoparticles Enhances Immunotherapy in Lung Cancer [ ACS Appl Mater Interfaces, 2022, 14(21):24144-24159] PubMed: 35579575
Effects of Supplemental Drugs on Hexaminolevulinate (HAL)-Induced PpIX Fluorescence in Bladder Cancer Cell Suspensions [ Int J Mol Sci, 2022, 23(14)7631] PubMed: 35886979

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。