Hydroxyurea

製品コードS1896 バッチS189606

印刷

化学情報

Synonyms

NCI-C04831, Hydroxycarbamide,NSC-32065

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

CH₄N₂O₂

分子量

76.05

CAS No.

127-07-1

Solubility (25°C)*

体外

DMSO

15 mg/mL (197.23 mM)

Water

15 mg/mL (197.23 mM)

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Hydroxyurea is an antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase. Hydroxyurea activates apoptosis and autophagy. Hydroxyurea is used to treat HIV infection.
in vitro	hydroxyurea can inhibit HIV-1 replication. In vitro experiments have shown that the 90% inhibitory concentration (IC90) of this compound for laboratory strains of HIV-1 in activated PBMC is 0.4 mM. It was also found to be synergistic with the nucleoside reverse transcriptase inhibitor didanosine and to inhibit HIV-1 replication in activated PBMC; this inhibition may be due to a reduction in deoxynucleoside triphosphate pool sizes. This chemical has been shown to sensitize didanosine-resistant mutants^[1]^[2]. It has demonstrated activity in the treatment of sickle cell anemia by increasing the production of fetal hemoglobin, which reduces hemolysis in patients with this disease. This agent exerts its cytostatic effect through inhibition of ribonucleotide reductase—the rate-limiting enzyme responsible for the conversion of ribonucleotides to deoxyribonucleotides, which are essential for DNA synthesis. As a result, cellular division is arrested in the S phase^[1].

製品説明

Hydroxyurea is an antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase. Hydroxyurea activates apoptosis and autophagy. Hydroxyurea is used to treat HIV infection.

in vitro

hydroxyurea can inhibit HIV-1 replication. In vitro experiments have shown that the 90% inhibitory concentration (IC90) of this compound for laboratory strains of HIV-1 in activated PBMC is 0.4 mM. It was also found to be synergistic with the nucleoside reverse transcriptase inhibitor didanosine and to inhibit HIV-1 replication in activated PBMC; this inhibition may be due to a reduction in deoxynucleoside triphosphate pool sizes. This chemical has been shown to sensitize didanosine-resistant mutants^[1]^[2]. It has demonstrated activity in the treatment of sickle cell anemia by increasing the production of fetal hemoglobin, which reduces hemolysis in patients with this disease. This agent exerts its cytostatic effect through inhibition of ribonucleotide reductase—the rate-limiting enzyme responsible for the conversion of ribonucleotides to deoxyribonucleotides, which are essential for DNA synthesis. As a result, cellular division is arrested in the S phase^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Erythroid cells
	濃度	30 μM
	反応時間	96 h
	実験の流れ	Erythroid cells obtained from peripheral blood of the same patients(Thirteen β-Thal/HbE patients are treated with hydroxyurea orally for 2 years at a starting dose of 5 mg/kg/day for 5 days/week with escalation to a maximum of 10 mg/kg/day) 1 year after they had stopped this compound treatment are treated with this chemical in vitro.Treatment of cells performs in primary culture with 30 μM this agent for 96 hours.
動物実験	動物モデル	Female athymic (nu/nu) nude mice(used for xenograft model)
	投薬量	1500 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/10860905/
https://pubmed.ncbi.nlm.nih.gov/10860899/
https://pubmed.ncbi.nlm.nih.gov/16338491/

https://pubmed.ncbi.nlm.nih.gov/12101433/

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カスタマーフィードバック

: Data from [Data independently produced by , , Blood, 2017, 130(26):2848-2859]

: Data from [Data independently produced by , , Int J Biochem Cell Biol, 2018, 105:1-12]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Inhibition of DEK restores hematopoietic stem cell function in Fanconi anemia [ J Exp Med, 2025, 222(3)e20241248]	PubMed: 39836085
Synergistic activity of RSL3 and Pyrimethamine to inhibit the proliferation of Plasmodium falciparum [ Antimicrob Agents Chemother, 2025, e0047125.]	PubMed: 40823867
Clofarabine Enhances the Transduction Efficiency of Recombinant AAV2 in the Retina [ Invest Ophthalmol Vis Sci, 2025, 66(5):42]	PubMed: 40434346
Coordinated inheritance of extrachromosomal DNAs in cancer cells [ Nature, 2024, 635(8037):201-209]	PubMed: 39506152
TOPORS E3 ligase mediates resistance to hypomethylating agent cytotoxicity in acute myeloid leukemia cells [ Nat Commun, 2024, 15(1):7360]	PubMed: 39198401
Multi-omics approaches reveal that diffuse midline gliomas present altered DNA replication and are susceptible to replication stress therapy [ Genome Biol, 2024, 25(1):319]	PubMed: 39707510
FOXP1 phosphorylation antagonizes its O-GlcNAcylation in regulating ATR activation in response to replication stress [ EMBO J, 2024, 44(2):457-483.]	PubMed: 39623140
Transposable element activity captures human pluripotent cell states [ EMBO Rep, 2024, 10.1038/s44319-024-00343-y]	PubMed: 39668246
Necrosulfonamide causes oxidation of PCM1 and impairs ciliogenesis and autophagy [ iScience, 2024, 27(4):109580]	PubMed: 38600973
Patient-derived rhabdomyosarcoma cells recapitulate the genetic and transcriptomic landscapes of primary tumors [ iScience, 2024, 27(10):110862]	PubMed: 39319271

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。