MPA (Medroxyprogesterone acetate)

製品コードS2567 バッチS256706

印刷

化学情報

Synonyms

NSC-26386, Medroxyprogesterone 17-acetate, Farlutin

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₄H₃₄O₄

分子量

386.52

CAS No.

71-58-9

Solubility (25°C)*

体外

DMSO

18 mg/mL (46.56 mM)

Ethanol (warmed with 50ºC water bath)

15 mg/mL (38.8 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 30%PEG300 2 5%Tween80 3 60%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.500mg/ml (1.29mM)	Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clarified DMSO stock solution to 300 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 600 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	MPA (Medroxyprogesterone acetate) is a synthetic progestin and act as a potent progesterone receptor agonist, used to treat abnormal menstruation or irregular vaginal bleeding.
in vitro	Medroxyprogesterone acetate (MPA) inhibits the enzyme 3-hydroxyste-roid dehydrogenase, involved in the reversible conversion between 5alpha-dihydroprogesterone (DHP) and 3alpha, 5alpha-tetrahydroprogesterone (THP), and therefore may affect the local actions of DHP and THP in the brain. ^[1] It reduces secretion of IL-6 and PTHrP from human breast cancer cells. This compound dose-dependently decreases the secretion and mRNA expression of IL-6 and PTHrP in the KTC-2 cells. ^[2] MPA and dexamethasone dose dependently increases alpha-ENaC promoter-driven luciferase activity in M-1 cells, which is not inhibited by Org31710, indicating that it regulates alpha-ENaC in a PR-independent manner. Medroxyprogesterone acetate and dexamethasone, but not progesterone, dose dependently increases alpha-ENaC and sgk1 mRNA in M-1 and in Madin-Darby canine kidney-C7 cells, both collecting duct cell lines. ^[3] At 0.1 nM, it significantly enhances the in vitro production of specific immunoglobulin G (IgG) antibodies, an effect that appears to involve the interaction of the progestin with PRG receptors^[4]
in vivo	Medroxyprogesterone acetate (MPA) impairs delayed memory retention on the water radial-arm maze (WRAM), and exacerbates overnight forgetting on the Morris maze (MM) in aged ovariectomized (OVX) rats. It significantly and progesterone marginally decreases GAD levels in the hippocampus, and both this compound and progesterone significantly increases GAD levels in the entorhinal cortex. ^[5]

製品説明

MPA (Medroxyprogesterone acetate) is a synthetic progestin and act as a potent progesterone receptor agonist, used to treat abnormal menstruation or irregular vaginal bleeding.

in vitro

Medroxyprogesterone acetate (MPA) inhibits the enzyme 3-hydroxyste-roid dehydrogenase, involved in the reversible conversion between 5alpha-dihydroprogesterone (DHP) and 3alpha, 5alpha-tetrahydroprogesterone (THP), and therefore may affect the local actions of DHP and THP in the brain. ^[1]

It reduces secretion of IL-6 and PTHrP from human breast cancer cells. This compound dose-dependently decreases the secretion and mRNA expression of IL-6 and PTHrP in the KTC-2 cells. ^[2]

MPA and dexamethasone dose dependently increases alpha-ENaC promoter-driven luciferase activity in M-1 cells, which is not inhibited by Org31710, indicating that it regulates alpha-ENaC in a PR-independent manner. Medroxyprogesterone acetate and dexamethasone, but not progesterone, dose dependently increases alpha-ENaC and sgk1 mRNA in M-1 and in Madin-Darby canine kidney-C7 cells, both collecting duct cell lines. ^[3]

At 0.1 nM, it significantly enhances the in vitro production of specific immunoglobulin G (IgG) antibodies, an effect that appears to involve the interaction of the progestin with PRG receptors^[4]

in vivo

Medroxyprogesterone acetate (MPA) impairs delayed memory retention on the water radial-arm maze (WRAM), and exacerbates overnight forgetting on the Morris maze (MM) in aged ovariectomized (OVX) rats. It significantly and progesterone marginally decreases GAD levels in the hippocampus, and both this compound and progesterone significantly increases GAD levels in the entorhinal cortex. ^[5]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	ISK cells
	濃度	29.3 µM
	反応時間	48 h
	実験の流れ	Cells were treated for 48 h with various concentrations of MPA (Medroxyprogesterone acetate).
動物実験	動物モデル	Wistar albino female rats
	投薬量	1, 2, 4, or 8 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/16758493/
https://pubmed.ncbi.nlm.nih.gov/12729473/
https://pubmed.ncbi.nlm.nih.gov/16189295/

https://pubmed.ncbi.nlm.nih.gov/11576224/
https://pubmed.ncbi.nlm.nih.gov/20074654/
https://pubmed.ncbi.nlm.nih.gov/33937078/

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カスタマーフィードバック

: Data from [Data independently produced by , , Molecular Pain, 2015, 11:46.]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Steroid-Modulated Transcription Synergistically Forms DNA Double-Strand Breaks With Topoisomerase II Inhibitor [ Cancer Sci, 2025, 10.1111/cas.70081]	PubMed: 40231641
Investigating Aging-Related Endometrial Dysfunction Using Endometrial Organoids [ Cell Prolif, 2024, e13780.]	PubMed: 39695355
Deficiency of UCHL1 results in insufficient decidualization accompanied by impaired dNK modulation and eventually miscarriage [ J Transl Med, 2024, 22(1):478]	PubMed: 38769534
Inhibition of the transmembrane transporter ABCB1 overcomes resistance to doxorubicin in patient-derived organoid models of HCC [ Hepatol Commun, 2024, 8(5)e0437]	PubMed: 38696353
The role of non-genomic actions of progesterone and its membrane receptor agonist in ovarian cancer cell death [ Cancer Rep (Hoboken), 2024, 7(1):e1934]	PubMed: 38013666
Genome-wide CRISPR screening reveals ADCK3 as a key regulator in sensitizing endometrial carcinoma cells to MPA therapy [ Br J Cancer, 2023, 129(4):601-611]	PubMed: 37402867
Genome-wide CRISPR screening reveals ADCK3 as a key regulator in sensitizing endometrial carcinoma cells to MPA therapy [ Br J Cancer, 2023, 129(4):601-611]	PubMed: 37402867
Elevated high-mannose N-glycans hamper endometrial decidualization [ iScience, 2023, 10.1016/j.isci.2023.108170]	PubMed: 37915610
RNA-seq and ATAC-seq analysis of CD163+ macrophage-induced progestin-insensitive endometrial cancer cells [ Cancer Med, 2023, 12(5):5964-5978]	PubMed: 36373483
RNA-seq and ATAC-seq analysis of CD163+ macrophage-induced progestin-insensitive endometrial cancer cells [ Cancer Med, 2023, 12(5):5964-5978]	PubMed: 36373483

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人間や獣医の診断であるか治療的な使用のためにでない。

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