Nitazoxanide

製品コードS1627 バッチS162703

印刷

化学情報

Chemical Structure Synonyms NTZ, NSC 697855 Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C12H9N3O5S
分子量 307.28 CAS No. 55981-09-4
Solubility (25°C)* 体外 DMSO 61 mg/mL (198.51 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 1.500mg/ml (4.88mM) Taking the 1 mL working solution as an example, add 50 μL of 30 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 1.000mg/ml (3.25mM) Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Nitazoxanide is a synthetic nitrothiazolyl-salicylamide derivative and an antiprotozoal agent(IC50 for canine influenza virus ranges from 0.17 to 0.21 μM). Nitazoxanide modulates autophagy and inhibits mTORC1 signaling.
in vitro

Nitazoxanide reduces parasite growth in cell culture by more than 90% with little evidence of drug-associated cytotoxicity. [1] This compound is a new thiazolide antiparasitic agent that shows excellent in vitro activity against a wide variety of protozoa and helminths. [2] It and its metabolite tizoxanide are more active in vitro than metronidazole against G. intestinalis, E. histolytica and T. vaginalis. [3] This agent exhibits potent inhibition of both HBV and HCV replication. It potentiates the effect of subsequent treatment with this compound plus IFN, but not this chemical plus 2'CmeC, in HCV replicon-containing cells. This chemical induces reductions in several HBV proteins (HBsAg, HBeAg, HBcAg) produced by 2.2.15 cells, but does not affect HBV RNA transcription. [4] It exhibits IC50, and IC90 values of 0.017 and 0.776 mg/mL respectively, against E. histolytica, 0.004 and 0.067 mg/mL against G. intestinalis, and 0.034 and 2.046 mg/mL against T. vaginalis. This compound is more toxic than metronidazole and albendazole against E. histolytica. [5]
in vivo

Nitazoxanide is partially effective at reducing the parasite burden in a gnotobiotic piglet diarrhea model when given orally for 11 days at 250 mg/kg/day but not at 125 mg/kg/day. This compound induces a drug-related diarrhea in piglets that might have influenced its therapeutic efficacy. [1]

プロトコル(参考用のみ)

参考

  • https://pubmed.ncbi.nlm.nih.gov/9687390/
  • https://pubmed.ncbi.nlm.nih.gov/15791519/
  • https://pubmed.ncbi.nlm.nih.gov/11751773/
  • https://pubmed.ncbi.nlm.nih.gov/17888524/
  • https://pubmed.ncbi.nlm.nih.gov/12120985/
  • https://pubmed.ncbi.nlm.nih.gov/22589723/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Unveiling transcriptional mechanisms of B7-H3 in breast cancer stem cells through proteomic approaches [ iScience, 2025, 28(4):112218] PubMed: 40230524
Coronavirus envelope protein activates TMED10-mediated unconventional secretion of inflammatory factors [ Nat Commun, 2024, 15(1):8708] PubMed: 39379362
PDIA3 orchestrates effector T cell program by serving as a chaperone to facilitate the non-canonical nuclear import of STAT1 and PKM2 [ Mol Ther, 2024, S1525-0016(24)00340-X] PubMed: 38822524
Nitazoxanide reduces inflammation and bone erosion in mice with collagen-induced arthritis via inhibiting the JAK2/STAT3 and NF-κB pathways in fibroblast-like synoviocytes [ Biomed Pharmacother, 2024, 171:116195] PubMed: 38262149
Transcriptional Differential Analysis of Nitazoxanide-Mediated Anticanine Parvovirus Effect in F81 Cells [ Viruses, 2024, 16(2)282] PubMed: 38400057
The MNRR1 activator nitazoxanide abrogates lipopolysaccharide-induced preterm birth in mice [ Placenta, 2023, 140:66-71] PubMed: 37544161
The MNRR1 activator nitazoxanide abrogates lipopolysaccharide-induced preterm birth in mice [ Placenta, 2023, 140:66-71] PubMed: 37544161
Utilizing Proteomic Techniques to Discover Host Protein Interactions with the E1 Glycoprotein of Venezuelan Equine Encephalitis Virus (VEEV) for Anti-Viral Discovery [ Virginia Tech, 2023, ] PubMed: None
Lipopolysaccharide induces placental mitochondrial dysfunction in murine and human systems by reducing MNRR1 levels via a TLR4-independent pathway [ iScience, 2022, 25(11):105342] PubMed: 36339251
Nitazoxanide and related thiazolides induce cell death in cancer cells by targeting the 20S proteasome with novel binding modes [ Biochem Pharmacol, 2022, 197:114913] PubMed: 35032461

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。