Rifampin (Rifampicin)

製品コードS1764 バッチS176402

印刷

化学情報

Synonyms

NSC-113926

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₄₃H₅₈N₄O₁₂

分子量

822.94

CAS No.

13292-46-1

Solubility (25°C)*

体外

DMSO

100 mg/mL (121.51 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Rifampin (Rifampicin) is a DNA-dependent RNA polymerase inhibitor, used to treat a number of bacterial infections.
in vitro	Rifampin (Rifampicin) inhibits IκBα degradation and mitogen-activated protein kinase (MAPK) phosphorylation. It is found to bind to human MD-2 in a concentration-dependent manner for both this compound and MD-2. The agent inhibits NF-κB activation induced by LPS (20 ng/ml) in a dose-dependent manner, with an IC50 of 44.1 μM in Blue hTLR4 293 cells (A) and immunocompetent microgial BV-2 cell. At 50 μM, it suppresses NF-κB activation at varying LPS doses, and the maximum NF-κB level induced by LPS in its presence is much lower than that in its absence. This compound inhibits NO production induced by LPS (200 ng/ml) in a dose-dependent manner in BV-2 cells, with an IC50 of 21.2 μM. It suppresses LPS induced TNF-α and IL-1β production in both microglia BV-2 and RAW 264.7 macrophage cells. Its inhibition of innate immune signaling is independent of the pregnane X receptor NR1I2. ^[1] When combined with polyester vascular prostheses (PVP) functionalised with cyclodextrin (PVP-CD), it results in significant bacterial adhesion reduction and growth inhibition against Gram-positive bacteria. ^[2] At 50 μg/mL, it significantly reduces the CFU counts of stationary-phase cultures and reduces the CFU counts of the log-phase culture to zero. It is particularly suitable since it is bactericidal and starts killing M. tuberculosis within an hour of exposure. ^[3]

製品説明

Rifampin (Rifampicin) is a DNA-dependent RNA polymerase inhibitor, used to treat a number of bacterial infections.

in vitro

Rifampin (Rifampicin) inhibits IκBα degradation and mitogen-activated protein kinase (MAPK) phosphorylation. It is found to bind to human MD-2 in a concentration-dependent manner for both this compound and MD-2. The agent inhibits NF-κB activation induced by LPS (20 ng/ml) in a dose-dependent manner, with an IC50 of 44.1 μM in Blue hTLR4 293 cells (A) and immunocompetent microgial BV-2 cell. At 50 μM, it suppresses NF-κB activation at varying LPS doses, and the maximum NF-κB level induced by LPS in its presence is much lower than that in its absence. This compound inhibits NO production induced by LPS (200 ng/ml) in a dose-dependent manner in BV-2 cells, with an IC50 of 21.2 μM. It suppresses LPS induced TNF-α and IL-1β production in both microglia BV-2 and RAW 264.7 macrophage cells. Its inhibition of innate immune signaling is independent of the pregnane X receptor NR1I2. ^[1] When combined with polyester vascular prostheses (PVP) functionalised with cyclodextrin (PVP-CD), it results in significant bacterial adhesion reduction and growth inhibition against Gram-positive bacteria. ^[2] At 50 μg/mL, it significantly reduces the CFU counts of stationary-phase cultures and reduces the CFU counts of the log-phase culture to zero. It is particularly suitable since it is bactericidal and starts killing M. tuberculosis within an hour of exposure. ^[3]

プロトコル(参考用のみ)

参考

https://pubmed.ncbi.nlm.nih.gov/23568774/
https://pubmed.ncbi.nlm.nih.gov/24140067/
https://pubmed.ncbi.nlm.nih.gov/11053379/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Leveraging the Aggregated Protein Dye YAT2150 for Malaria Chemotherapy [ Pharmaceutics, 2024, 16(10)1290]	PubMed: 39458619
Studying Target-Engagement of Anti-Infectives by Solvent-Induced Protein Precipitation and Quantitative Mass Spectrometry [ ACS Infect Dis, 2024, 10(12):4087-4102]	PubMed: 39566904
Pralatrexate represses the resistance of HCC cells to molecular targeted agents via the miRNA-34a/Notch pathway [ Discov Oncol, 2024, 15(1):709]	PubMed: 39585461
TPX2 enhances the transcription factor activation of PXR and enhances the resistance of hepatocellular carcinoma cells to antitumor drugs [ Cell Death Dis, 2023, 14(1):64]	PubMed: 36707511
Low-Dose Rifabutin Increases Cytotoxicity in Antimitotic-Drug-Treated Resistant Cancer Cells by Exhibiting Strong P-gp-Inhibitory Activity [ Int J Mol Sci, 2022, 23(13)7383]	PubMed: 35806386
SARS-CoV-2 RdRp Inhibitors Selected from a Cell-Based SARS-CoV-2 RdRp Activity Assay System [ Biomedicines, 2021, 9(8)996]	PubMed: 34440200
MDM2 Binding Protein Induces the Resistance of Hepatocellular Carcinoma Cells to Molecular Targeting Agents via Enhancing the Transcription Factor Activity of the Pregnane X Receptor [ Front Oncol, 2021, 11:715193]	PubMed: 34249768
Neuroprotective effects of some epigenetic modifying drugs' on Chlamydia pneumoniae-induced neuroinflammation: A novel model [ PLoS One, 2021, 16(11):e0260633]	PubMed: 34847172
Identification of Small-Molecule Inhibitors of Brucella Diaminopimelate Decarboxylase by Using a High-Throughput Screening Assay. [ Front Microbiol, 2020, 10:2936]	PubMed: 32038511
A methylation functional detection hepatic cell system validates correlation between DNA methylation and drug-induced liver injury. [ Pharmacogenomics J, 2020, 10.1038/s41397-020-0160-7]	PubMed: 32029904

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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