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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | Na3O4V |
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分子量 | 183.91 | CAS No. | 13721-39-6 | |
Solubility (25°C)* | 体外 | Water | 36 mg/mL (195.74 mM) | |
DMSO | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Sodium orthovanadate is an alkaline phosphatase and (Na,K)-ATPase inhibitor with IC50 of 10 μM. |
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in vitro | In transient forebrain ischemia, Sodium orthovanadate rescues cells from delayed neuronal death in the hippocampal CA1 region. The neuroprotective effects of Sodium orthovanadate and IGF-1 are associated with preventing decreased Akt-Ser-473 phosphorylation in the CA1 region observed immediately after reperfusion. Akt is moderately activated in the cell bodies and dendrites of pyramidal neurons after orthovanadate treatment. The Sodium orthovanadate treatment also prevents the decrease in phosphorylation of mitogen-activated protein kinase (MAPK). [3] Sodium orthovanadate inhibits ASK1 through the PI3-K/Akt-dependent pathway. [4] Sodium orthovanadate up-regulates Akt activity in the brain and in turn rescue neurons from delayed neuronal death by inhibiting FKHR-dependent or -independent death signals in neurons. [4] |
in vivo | In a rat model of myocardial ischemic infarction, sodium orthovanadate rescues cells from ischemia/reperfusion injuries. Post-treatment with Sodium orthovanadate reduces infarct size in a dose-dependent manner. [5] Sodium orthovanadate treatment also ameliorates contractile dysfunction of the left ventricle 72 hours after reperfusion. The cytoprotective action of Sodium orthovanadate treatment is closely associated with inhibition of fodrin breakdown. Sodium orthovanadate treatment inhibits caspase-3 activation induced by ischemia. [6] |
キナーゼアッセイ | ATPase assay | |
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The coupled assay linking ADP production to NADH oxidation is used for all ATPase assays. The fraction of active enzyme is determined by measuring enzyme activity after linearity has been achieved by the activity prior to addition of Sodium orthovanadate. Final steady state hydrolysis rates are independent of the order addition of Sodium orthovanadate and enzyme. | ||
動物実験 | 動物モデル | Male Sprague-Dawley rats |
投薬量 | 0.005 mL/min/100g of b.wt. over 20 minutes | |
投与方法 | Administered via i.v. |
The LCK-14-3-3ζ-TRPM8 axis regulates TRPM8 function/assembly and promotes pancreatic cancer malignancy [ Cell Death Dis, 2022, 13(6):524] | PubMed: 35665750 |
Hexachlorophene, a selective SHP2 inhibitor, suppresses proliferation and metastasis of KRAS-mutant NSCLC cells by inhibiting RAS/MEK/ERK and PI3K/AKT signaling pathways [ Toxicol Appl Pharmacol, 2022, 441:115988] | PubMed: 35307375 |
Pore-forming alpha-hemolysin efficiently improves the immunogenicity and protective efficacy of protein antigens [ PLoS Pathog, 2021, 17(7):e1009752] | PubMed: 34288976 |
Design of ratiometric monoaromatic fluorescence probe via modulating intramolecular hydrogen bonding: A case study of alkaline phosphatase sensing [ Anal Chim Acta, 2021, 1143:144-156] | PubMed: 33384112 |
SARS-CoV-2 Membrane Glycoprotein M Triggers Apoptosis With the Assistance of Nucleocapsid Protein N in Cells [ Front Cell Infect Microbiol, 2021, 11:706252] | PubMed: 34513728 |
Interleukin 16 contributes to gammaherpesvirus pathogenesis by inhibiting viral reactivation [ PLoS Pathog, 2020, 16(7):e1008701] | PubMed: 32735617 |
[ Cancer Immunol Res, 2019, ] | PubMed: 30401677 |
Displacement Induced Off-On Fluorescent Biosensor Targeting IDO1 Activity in Live Cells. [ Anal Chem, 2019, 91(23):14943-14950] | PubMed: 31714063 |
Characterization of Semaphorin 6A-Mediated Effects on Angiogenesis Through Regulation of VEGF Signaling. [ Methods Mol Biol, 2017, 1493:345-361] | PubMed: 27787863 |
Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats [Liu MW, et al. Int J Mol Med, 2015, 36(2):386-98] | PubMed: 26063084 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。