Sulfasalazine

製品コードS1576 バッチS157605

印刷

化学情報

Synonyms

NSC 667219, Sulphasalazine

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₈H₁₄N₄O₅S

分子量

398.39

CAS No.

599-79-1

Solubility (25°C)*

体外

DMSO

80 mg/mL (200.8 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	8.300mg/ml (20.83mM)	Taking the 1 mL working solution as an example, add 50 μL of 166 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.250mg/ml (0.63mM)	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Sulfasalazineはメサラジンのスルファ誘導体であり、抗炎症剤として腸疾患や関節リウマチの治療に用いられます。Sulfasalazineはnuclear factor kappa B (NF-κB)、TGF-β、およびCOX-2の強力かつ特異的な阻害剤です。Sulfasalazineはferroptosis、apoptosis、およびautophagyを誘発します。
in vitro	Sulfasalazine, like methotrexate, enhances adenosine release at an inflamed site and that adenosine diminishes inflammation via occupancy of A2 receptors on inflammatory cells. This compound treatment for 4 hours inhibits kappaB-dependent transcription with an IC50 value of approximately 0.625 mM. This chemical (2.5 mM) results in cell death of T-lymphocytes in a dose- and time-dependent manner. This compound but not 5ASA or sulfapyridine, strongly inhibits NF-kappaB activation and potently induces apoptosis in T-lymphocytes. It is cleaved into sulfapyridine and 5-aminosalicylic acid (5-ASA; mesalamine) by colonic bacteria, and the latter, too, is reported to suppress NF-kappaB activity. This compound but not its cleaved form 5-ASA causes a dose-dependent inhibition of glioma growth, this effect is entirely attributable to the inhibition of cystine uptake via the system x(c)(-) cystine-glutamate transporter. It inhibits cystine uptake causing a chronic depletion of intracellular GSH and consequently compromised cellular redox defense which stymied tumor growth.
in vivo	Sulfasalazine markedly decreases the number of leukocytes that accumulated in the inflamed (carrageenan, 2 mg/ml) air pouch in the murine air pouch model of inflammation. This compound treatment promotes a marked increase in splenocyte 5-aminoimidazole-4-carboxamidoribonucleotide (AICAR) concentration, which is consistent with the in vitro observation that it inhibits AICAR transformylase.

プロトコル(参考用のみ)

参考

https://pubmed.ncbi.nlm.nih.gov/8596047/
https://pubmed.ncbi.nlm.nih.gov/10602313/
https://pubmed.ncbi.nlm.nih.gov/19457125/

https://pubmed.ncbi.nlm.nih.gov/22381741/

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Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

A drug repurposing study identifies novel FOXM1 inhibitors with in vitro activity against breast cancer cells [ Med Oncol, 2024, 41(8):188]	PubMed: 38918225
Ferroptosis-Enhanced Immunotherapy with an Injectable Dextran-Chitosan Hydrogel for the Treatment of Malignant Ascites in Hepatocellular Carcinoma [ Adv Sci (Weinh), 2023, 10(20):e2300517]	PubMed: 37132587
Mitochondrial outer membrane protein FUNDC2 promotes ferroptosis and contributes to doxorubicin-induced cardiomyopathy [ Proc Natl Acad Sci U S A, 2022, 119(36):e2117396119]	PubMed: 36037337
AUF1 protects against ferroptosis to alleviate sepsis-induced acute lung injury by regulating NRF2 and ATF3 [ Cell Mol Life Sci, 2022, 79(5):228]	PubMed: 35391558
Intratarget Microdosing for Deep Phenotyping of Multiple Drug Effects in the Live Brain [ Front Bioeng Biotechnol, 2022, 10:855755]	PubMed: 35372313
High-throughput in situ perturbation of metabolite levels in the tumor micro-environment reveals favorable metabolic condition for increased fitness of infiltrated T-cells [ Front Cell Dev Biol, 2022, 10:1032360]	PubMed: 36619865
Peroxidation of n-3 and n-6 polyunsaturated fatty acids in the acidic tumor environment leads to ferroptosis-mediated anticancer effects [ Cell Metab, 2021, S1550-4131(21)00233-3]	PubMed: 34118189
DCN released from ferroptotic cells ignites AGER-dependent immune responses [ Autophagy, 2021, 10.1080/15548627.2021.2008692]	PubMed: 34964698
PARP inhibition promotes ferroptosis via repressing SLC7A11 and synergizes with ferroptosis inducers in BRCA-proficient ovarian cancer [ Redox Biol, 2021, S2213-2317(21)00076-8]	PubMed: 33722571
STING1 Promotes Ferroptosis Through MFN1/2-Dependent Mitochondrial Fusion [ Front Cell Dev Biol, 2021, 9:698679]	PubMed: 34195205

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。