TAME

製品コードS2225 バッチS222501

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C14H22N4O4S
分子量 342.41 CAS No. 901-47-3
Solubility (25°C)* 体外 DMSO 69 mg/mL (201.51 mM)
Water 69 mg/mL (201.51 mM)
Ethanol 3 mg/mL (8.76 mM)
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 3.45mg/ml (10.08mM) Taking the 1 mL working solution as an example, add 50 μL of 69 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution
5% DMSO 95% Corn oil

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 0.49mg/ml (1.43mM) Taking the 1 mL working solution as an example, add 50 μL of 9.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Tosyl-L-Arginine Methyl Ester (TAME) はAPC阻害剤です。
in vitro TAME inhibits cyclin proteolysis in mitotic Xenopus egg extract with IC50 of 12 µM. This compound at concentration of 1-200 μM arrests interphase extract treated with recombinant cyclin B1/Cdc2 complex in mitosis, with stable cyclin B1 and phosphorylated Cdc27. It at concentration of 200 μM dramaticly inhibits the ubiquitin ligase activity of the Anaphase-Promoting Complex (APC), accompanied by reduced binding of Cdh1 to APC. This chemical addition to interphase extract reduces Cdc20 association with the APC in a dose-dependent manner partly by binding directly to APC, and the contribution motif is the C-terminal isoleucine-arginine (IR) tail on APC. It is hydrolysed by trypsin with Km of 0.328 mM. This compound accelerates the ATP hydrolysis process about 12-fold. It interacts with β and γ phosphate and the adenine ring of ATP by the guanidinium group and the aromatic ring. This chemical at concentration of 50 mM inhibits nutrient-induced germination and pressure-induced germination at 600 MPa in Bacillus subtilis. It induces a concentration dependent contractile response on ileal strips with EC50 of 4.3 x 103 M.

プロトコル(参考用のみ)

キナーゼアッセイ 3H-TAME binding assay
3H-TAME (200 nM; 15 Ci/mmol) is added to 100 μl interphase Xenopus extract or HeLa cell lysate. APC is immunoprecipitated with Cdc27 antibody coupled to affiprep beads. The beads are washed with XB and radioactivity measured by scintillation counting. Alternatively, this compound is added and Cdc27 immunoprecipitation is performed after one or two rounds of APC immunodepletion. Specific binding is calculated as the difference between counts associated with Cdc27 antibody beads compared to beads lacking antibody (mock IP

参考

  • https://pubmed.ncbi.nlm.nih.gov/20951947/
  • http://www.sciencedirect.com/science/article/pii/S0308814609012515
  • http://www.springerlink.com/content/1310623880656547/
  • https://pubmed.ncbi.nlm.nih.gov/18274689/
  • https://pubmed.ncbi.nlm.nih.gov/10618233/
  • https://pubmed.ncbi.nlm.nih.gov/9731476/

カスタマーフィードバック

Data from [Data independently produced by , , Oncotarget, 2016, 7(18):25478-92]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma [ Dev Cell, 2023, 58(3):192-210.e11] PubMed: 36696903
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Establishment and characterization of NCC-UPS4-C1: a novel cell line of undifferentiated pleomorphic sarcoma from a patient with Li-Fraumeni syndrome [ Hum Cell, 2022, 10.1007/s13577-022-00671-y] PubMed: 35118583
TRPS1 drives heterochromatic origin refiring and cancer genome evolution [ Cell Rep, 2021, 34(10):108814] PubMed: 33691114
Establishment and characterization of NCC-MFS4-C1: a novel patient-derived cell line of myxofibrosarcoma [ Hum Cell, 2021, 34(6):1911-1918] PubMed: 34383271
Establishment and characterization of the NCC-GCTB4-C1 cell line: a novel patient-derived cell line from giant cell tumor of bone [ Hum Cell, 2021, 10.1007/s13577-021-00639-4] PubMed: 34731453
Establishment and characterization of novel patient-derived cell lines from giant cell tumor of bone [ Hum Cell, 2021, 10.1007/s13577-021-00579-z] PubMed: 34304386
Benzo[a]pyrene represses DNA repair through altered E2F1/E2F4 function marking an early event in DNA damage-induced cellular senescence [ Nucleic Acids Res, 2020, gkaa965] PubMed: 33166399
Establishment and characterization of patient-derived cancer models of malignant peripheral nerve sheath tumors. [ Cancer Cell Int, 2020, 19;20:58] PubMed: 32099531
Establishment and characterization of NCC-MFS2-C1: a novel patient-derived cancer cell line of myxofibrosarcoma [ Hum Cell, 2020, 10.1007/s13577-020-00420-z] PubMed: 32870449

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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