Acetazolamide

製品コードS4506 バッチS450602

化学情報

	Synonyms	Diamox	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₄H₆N₄O₃S₂
	分子量	222.25	CAS No.	59-66-5
Solubility (25°C)*	体外	DMSO	44 mg/mL (197.97 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Acetazolamide (Diamox), a potent carbonic anhydrase (CA) inhibitor, is commonly used in clinical practice as an immediate and readily available option for acute reduction of intraocular pressure(IOP).
in vitro	acetazolamide reduces invasiveness of cancer cells in vitro^[3].
in vivo	Acetazolamide reduces inflammation-induced heat hyperalgesia, probably acting both peripherally and centrally^[1]. Acetazolamide shows significant inhibitory effect on angiogenesis in CAM and endothelial cell proliferation^[2]. Acetazolamide is clinically used to reduce intraocular pressure in glaucoma, to correct metabolic alkalosis, and to manage cerebral edema. Its treatment may also be beneficial as an adjunct to cancer chemotherapy, because it can produce additive tumor growth delays with anticancer drugs in vivo^[3].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	renal carcinoma cell lines(Caki-1, Caki-2, ACHN, and A-498)
	濃度	10 μM
	反応時間	48 h
	実験の流れ	The invasion assay is performed in a Biocoat Matrigel invasion chamber. Cells (n = 1.8 × 10⁵) are added to the chamber and cultured for 48 h in the presence or absence of acetazolamide, which was administered twice during the 48-h period: the first dose at the beginning of the assay and the second after 24 h. After growing for 48 h, noninvasive cells are removed from the upper surface of the membrane with a cotton swab. The invasive cells on the lower surface of the membrane are fixed with 4% (vol/vol) neutral-buffered formaldehyde, stained with toluidine blue, and counted in 20 separate areas with a Leitz Diaplan microscope.
動物実験	動物モデル	Male Sprague-Dawley rats
	投薬量	10, 100, 200 mg/kg
	投与方法	i.p.

参考

[4] Hardman, J.G., et al. Goodman and Gilman's The Pharmacological Basis of Therapeutics. 9th ed. New York, NY: McGraw-Hill, 1996, p. 693.

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Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Eleutheroside B ameliorated high altitude pulmonary edema by attenuating ferroptosis and necroptosis through Nrf2-antioxidant response signaling [ Biomed Pharmacother, 2022, 156:113982]	PubMed: 36411652
The carbonic anhydrase inhibitor acetazolamide inhibits urinary bladder cancers via suppression of β-catenin signaling [ Cancer Sci, 2022, 113(8):2642-2653]	PubMed: 35723039
Meldonium Ameliorates Hypoxia-Induced Lung Injury and Oxidative Stress by Regulating Platelet-Type Phosphofructokinase-Mediated Glycolysis [ Front Pharmacol, 2022, 13:863451]	PubMed: 35450040
Effect of cGMP-activated aquaporin 1 on TRPV4 in rats with allodynia induced by chronic compression of the dorsal root ganglion. [ Neurosci Lett, 2019, 10.1016/j.neulet.2019.134630]	PubMed: 31790718

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人間や獣医の診断であるか治療的な使用のためにでない。

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