Datasheet

生物学的記述

Specificity	Acetyl-α-Tubulin (Lys40) Antibody [F22D16] detects endogenous levels of total α-Tubulin protein only when it is Acetylated at Lys40.
Background	Acetyl-α-tubulin (Lys40) represents the lysine 40-acetylated form of α-tubulin, a core subunit of the tubulin heterodimer family that polymerizes into microtubules, essential cytoskeletal elements in eukaryotic cells. Acetylation targets the ε-amino group of the highly conserved Lys40 residue within a flexible 20-amino-acid luminal loop (residues 37-56) that protrudes into the microtubule interior, where it folds into an irregular turn packing against the acetyltransferase TAT active site funnel formed by conserved residues like Gln58, Arg158, Ile64, and Asp157 for substrate recognition and deprotonation. This internal modification, unique among microtubule PTMs, occurs post-polymerization via TAT accessing the lumen through lattice openings. Lys40 acetylation enhances microtubule flexibility and resilience to mechanical stress by altering protofilament rotational angles and lattice stability, promoting long-lived microtubules critical for dendritic arborization, axon growth, intracellular transport, cell motility, and neuronal branching. It is dynamically reversed by HDAC6 deacetylation, integrating into mechanosensitive pathways where stable acetylated MTs support ciliary/flagellar beating and polarity establishment. Dysregulation links to neurodegeneration, as reduced acetylation impairs dendritic stability and locomotion in models like Drosophila atat mutants. .

Specificity

Acetyl-α-Tubulin (Lys40) Antibody [F22D16] detects endogenous levels of total α-Tubulin protein only when it is Acetylated at Lys40.

Background

Acetyl-α-tubulin (Lys40) represents the lysine 40-acetylated form of α-tubulin, a core subunit of the tubulin heterodimer family that polymerizes into microtubules, essential cytoskeletal elements in eukaryotic cells. Acetylation targets the ε-amino group of the highly conserved Lys40 residue within a flexible 20-amino-acid luminal loop (residues 37-56) that protrudes into the microtubule interior, where it folds into an irregular turn packing against the acetyltransferase TAT active site funnel formed by conserved residues like Gln58, Arg158, Ile64, and Asp157 for substrate recognition and deprotonation. This internal modification, unique among microtubule PTMs, occurs post-polymerization via TAT accessing the lumen through lattice openings. Lys40 acetylation enhances microtubule flexibility and resilience to mechanical stress by altering protofilament rotational angles and lattice stability, promoting long-lived microtubules critical for dendritic arborization, axon growth, intracellular transport, cell motility, and neuronal branching. It is dynamically reversed by HDAC6 deacetylation, integrating into mechanosensitive pathways where stable acetylated MTs support ciliary/flagellar beating and polarity establishment. Dysregulation links to neurodegeneration, as reduced acetylation impairs dendritic stability and locomotion in models like Drosophila atat mutants. .

使用情報

Application

WB, IHC

Dilution

WB

1:15000-1:30000

Reactivity

bovine, frog, invertebrates, human, hamster, mouse, protista, pig, monkey, chicken, rat, plant

Source

Mouse Monoclonal Antibody

MW

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/36827311/
https://pubmed.ncbi.nlm.nih.gov/24906155/

Acetyl-α-Tubulin (Lys40) Antibody [F22D16]

生物学的記述

使用情報

References

Application Data